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Plasmid#61420PurposeExpresses human OTUB1 (full-length) in E. coli.DepositorInsertOTUB1 (Ubiquitin thioesterase OTUB1) (OTUB1 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationPromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2plus-mCerulean 156-239-GGGS
Plasmid#162616PurposeAllows for transcription of control mCerulean fluorophore half for injection into zebrafish embryos for BiFC assaysDepositorInsertmCerulean
UseTagsExpressionMammalianMutation156-239PromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-mito-CAR-GECO1
Plasmid#46022PurposeCarmine intensiometric genetically encoded Ca2+-indicators for optical imagingDepositorInsertCAR-GECO1
UseTagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationR-GECO1 E163V/I166V/V174T/M176I/F222I/A302PPromoterCMVAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-E2F7-T2A-PuroR
Plasmid#162348PurposeLentiviral expression of E2F7 under the control of the TetON promoterDepositorInsertE2F7 (E2F7 Human)
UseLentiviralTagsT2A-PuroRExpressionMammalianMutationPromoterTet-ONAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2plus-mVenus1-155-I152L-GGGS
Plasmid#162613PurposeAllows for transcription of improved control mVenus I152L fluorophore half for injection into zebrafish embryos for BiFC assaysDepositorInsertmVenus
UseTagsExpressionMammalianMutation1-155-I152LPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-LHX8-T2A-PuroR
Plasmid#162350PurposeLentiviral expression of LHX8 under the control of the TetON promoterDepositorInsertLHX8 (LHX8 Human)
UseLentiviralTagsT2A-PuroRExpressionMammalianMutationPromoterTet-ONAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-KLF7-T2A-PuroR
Plasmid#162346PurposeLentiviral expression of KLF7 under the control of the TetON promoterDepositorInsertKLF7 (KLF7 Human)
UseLentiviralTagsT2A-PuroRExpressionMammalianMutationPromoterTet-ONAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD6B (full-length, aa 1-293)
Plasmid#61418PurposeExpresses human OTUD6B (full-length) in E. coli.DepositorInsertOTUD6B (OTUD6B Human)
UseTagsHis6-GST-3CExpressionBacterialMutationPromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSHUT4-eYFP
Plasmid#133094PurposeAgrobacterium T-DNA overexpression cassette comprising constitutive TEF-1alpha fused to reporter eYFP. Integrates near beta-tubulin gene in F. solani genome. Replace eYFP w/ XhoI BamHIDepositorInsertenhanced yellow flourescent protein
UseFungal expressionTagsExpressionMutationPromoterTEF-1αAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD5 (OTU, aa 171-358)
Plasmid#61415PurposeExpresses human OTUD5 (OTU domain) in E. coli.DepositorInsertOTUD5 (OTUD5 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-170 and aa 359-571.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRK5 FLAG wildtype, catalytic dead lipin 1
Plasmid#32006DepositorInsertLipin1 (Lpin1 Mouse)
UseTagsFLAGExpressionMammalianMutationD712E and D714EPromoterCMV, SP6Available sinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
GA-DEVD-GB-NES
Plasmid#61020Purposegreen ddFP based caspase-3 biosensorDepositorInsertddGFP A and ddGFP B
UseTagsHindIII and NES sequence LALKLAGLDIGS placed afte…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD3 (OTU+UBA, aa 52-275)
Plasmid#61411PurposeExpresses human OTUD3 (OTU+UBA domains) in E. coli.DepositorInsertOTUD3 (OTUD3 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-51 and aa 276-398.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-Cezanne2 (OTU+UBA, aa 1-462)
Plasmid#61582PurposeExpresses human Cezanne2 (OTU+UBA domains) in E. coli.DepositorInsertCezanne2 (OTUD7A Human)
UseTagsHis6-GST-3CExpressionBacterialMutationIsoform 2. Deleted aa 463-933.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
Flag‐mTim3
Plasmid#49208Purposeexpresses Flag tagged mouse Tim3DepositorInsertTim3 (Havcr2 Mouse)
UseTagsFLAG and signal sequenceExpressionMammalianMutationno Tim3 signal sequencePromoterEF1aAvailable sinceDec. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-LmoCas6
Plasmid#126485PurposeEncodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertLmoCas6
UseCRISPRTagsFlag, NLSExpressionMammalianMutationPromoterCMV and U6Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-VCPIP (OTU, aa 25-561)
Plasmid#61583PurposeExpresses human VCPIP (OTU domain) in E. coli.DepositorInsertVCPIP (VCPIP1 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-24 and aa 562-1222.PromoterT7Available sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2plus-mKate2
Plasmid#162624PurposeAllows for transcription of mKate2 control for injection into zebrafish embryos for BiFC assaysDepositorInsertmKate2
UseTagsExpressionMammalianMutationPromoterSP6Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-LmoCas8b2
Plasmid#126482PurposeEncodes L.monocytogenes CRISPR Type I-B Cas8b2 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertLmoCas8b2
UseCRISPRTagsFlag, NLSExpressionMammalianMutationPromoterCMV and U6Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-LmoCas7
Plasmid#126483PurposeEncodes L.monocytogenes CRISPR Type I-B Cas7 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertLmoCas7
UseCRISPRTagsFlag, NLSExpressionMammalianMutationPromoterCMV and U6Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only