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Plasmid#117527PurposeLevel0 Golden Gate part: CDS, Sp-eCas9 1.0 (no stop codon)DepositorInsertSp-eCas9 1.0 (no stop codon)
UseCRISPRTagsExpressionMutationK810A, K1003A, R1060APromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0015
Plasmid#117525PurposeLevel0 Golden Gate part: CDS, SpCas9-KA (no stop codon)DepositorInsertSpCas9-KA (no stop codon)
UseCRISPRTagsExpressionMutationK855APromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0001
Plasmid#117523PurposeLevel0 Golden Gate part: CDS, SpCas9-DE (no stop codon)DepositorInsertSpCas9-DE (no stop codon)
UseCRISPRTagsExpressionMutationD1135EPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0050
Plasmid#117617PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_AtMPK19 {AT3G14720}(pEPOR1CB0081)DepositorInsert[35S:Sp-eCas9 1.0(pEPOR1CB0010) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0049
Plasmid#117616PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtMPK19 {AT3G14720}(pEPOR1CB0081)DepositorInsert[35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0051
Plasmid#117618PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_AtMPK19 {AT3G14720}(pEPOR1CB0081)DepositorInsert[35S:Sp-eCas9 1.1(pEPOR1CB0011) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 N4A 3xFLAG
Plasmid#111975PurposeShuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker. It has 3xFLAG at the C-terminus. Contains the N4A mutation.DepositorInsertHsh155 N4A 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationT178A R181A R182A R186APromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 K818A 3xFLAG
Plasmid#111982PurposeShuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains K818A mutation. LethalDepositorInsertHsh155 K818A 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationK818APromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 R775A 3xFLAG
Plasmid#111977PurposeShuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains R775A mutation. LethalDepositorInsertHsh155 R775A 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationR775APromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 R778A 3xFLAG
Plasmid#111979PurposeShuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains R778A mutation. LethalDepositorInsertHsh155 R778A 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationR778APromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS413 Prp5 AAAA
Plasmid#87600PurposeEncodes Prp5 AAAA for expression in yeastDepositorInsertPRP5 (PRP5 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationD136A, P137A, L138A, D139APromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS413 Prp5 TAG
Plasmid#87603PurposeEncodes Prp5 SAT motif to TAG for expression in yeastDepositorInsertPRP5 (PRP5 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationS446T, T448GPromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGADT7 Hsh155 K335R
Plasmid#87299PurposeEncodes Y2H Gal4 activation domain for Hsh155 K335RDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsGAL4 AD and HAExpressionBacterial and YeastMutationK335RPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBKT7-CLF1
Plasmid#87548PurposeEncodes Y2H Gal4 DNA binding domain for Clf1DepositorInsertCLF1 (CLF1 Budding Yeast)
UseTagsGAL4 BD and MycExpressionBacterial and YeastMutationPromoterAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGADT7 Hsh155 D450G
Plasmid#87303PurposeEncodes Y2H Gal4 activation domain for Hsh155 D450GDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsGAL4 ADExpressionBacterial and YeastMutationD450GPromoterAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGADT7 Hsh155 P369K
Plasmid#87302PurposeEncodes Y2H Gal4 activation domain for Hsh155 P369KDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsGAL4 ADExpressionBacterial and YeastMutationP369KPromoterAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGADT7 Hsh155 H331Q
Plasmid#87296PurposeEncodes Y2H Gal4 activation domain for Hsh155 H331QDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsGAL4 ADExpressionBacterial and YeastMutationH331QPromoterAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGADT7 Hsh155 H331D
Plasmid#87295PurposeEncodes Y2H Gal4 activation domain for Hsh155 H331DDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsGAL4 ADExpressionBacterial and YeastMutationH331DPromoterAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGADT7 Hsh155 N295D
Plasmid#87294PurposeEncodes Y2H Gal4 activation domain for Hsh155 N295DDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsGAL4 ADExpressionBacterial and YeastMutationN295DPromoterAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGADT7 Hsh155 R294L
Plasmid#87293PurposeEncodes Y2H Gal4 activation domain for Hsh155 R294LDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsGAL4 ADExpressionBacterial and YeastMutationR294LPromoterAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only