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Showing: 81 - 100 of 234 results

  3. pBactin-mOrange2-Cdk5rap2-CTD

    Plasmid
    #196855
    Purpose
    Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    mOrange2-Cdk5rap2-CTD (Cdk5rap2 Discosoma sp.)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    None (wt)
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Availability
    Academic Institutions and Nonprofits only
    196855-plasmid-map-sequence-id-391813

    Enlarge

  5. pBactin-Nedd1-mOrange2-rCM1

    Plasmid
    #196861
    Purpose
    Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC.
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    Nedd1-mOrange2-rCM1 (Nedd1 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Availability
    Academic Institutions and Nonprofits only
    196861-plasmid-map-sequence-id-391830

    Enlarge

  6. pBactin-Nedd1-mOrange2-F75A

    Plasmid
    #196862
    Purpose
    Expression of Need1-rCM1 harboring a (F75A) mutation in the rat (r) Centrosomin motif 1 (CM1) and fused to mOrange2. F75A mutatuon renders CM1 incapable of binding and activating gamma-TuRC
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    Nedd1-mOrange2-F75A (Nedd1 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    F75A in the CM1 domain
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Availability
    Academic Institutions and Nonprofits only
    196862-plasmid-map-sequence-id-391831

    Enlarge

  7. pBactin-Nedd1-mOrange2-hCM1

    Plasmid
    #196863
    Purpose
    Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    Nedd1-mOrange2-hCM1 (Nedd1 Human, Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Availability
    Academic Institutions and Nonprofits only
    196863-plasmid-map-sequence-id-391832

    Enlarge

  9. RHPMP126 (mOrange2-alpha Tubulin)

    Plasmid
    #88835
    Purpose
    Localization by Super-Resolution microscopy of alpha-Tubulin fused to mOrange2
    Depositor
    Ricardo Henriques
    Insert
    alpha-tubulin (TUBA1B) (TUBA1B Human)
    Use
    Tags
    mOrange2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    88835-plasmid-map-sequence-id-177488

    Enlarge

  10. RHPMP133 (mOrange2-beta Actin)

    Plasmid
    #88837
    Purpose
    Localization by Super-Resolution microscopy of beta-Actin fused to mOrange2
    Depositor
    Ricardo Henriques
    Insert
    beta-Actin (ACTB Human)
    Use
    Tags
    mOrange2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    88837-plasmid-map-sequence-id-177529

    Enlarge

  12. mOrange2-Lifeact-7

    Plasmid
    #54748
    Purpose
    Localization: Actin, Excitation: 549, Emission: 565
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    mOrange2-Lifeact
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  13. PS-mOrange2-Homer1-N-18

    Plasmid
    #57811
    Purpose
    Localization: Integrin/Focal Adhesions, Excitation: 548 / 634, Emission: 565 / 662
    Depositor
    Michael Davidson
    Insert
    Homer1
    Use
    Tags
    PS-mOrange2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    57811-plasmid-map-sequence-id-273037

    Enlarge

  14. PS-mOrange2-MyosinIIB-C-18

    Plasmid
    #57812
    Purpose
    Localization: Cytoskeleton, Excitation: 548 / 634, Emission: 565 / 662
    Depositor
    Michael Davidson
    Insert
    MyosinIIB (MYH10 Human)
    Use
    Tags
    PS-mOrange2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only

  16. mOrange2-CathespinB-6

    Plasmid
    #57945
    Purpose
    Localization: Lysosomes, Excitation: 549, Emission: 565
    Depositor
    Michael Davidson
    Insert
    CathespinB
    Use
    Tags
    mOrange2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    57945-plasmid-map-sequence-id-226429

    Enlarge

  17. mOrange2-CENPB-N-22

    Plasmid
    #57946
    Purpose
    Localization: Nucleus/Centromeres, Excitation: 549, Emission: 565
    Depositor
    Michael Davidson
    Insert
    CENPB (CENPB Human)
    Use
    Tags
    mOrange2
    Expression
    Mammalian
    Mutation
    aa 1-169 of CENPB (DNA Binding Domain)
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only

  19. mOrange2-C-Src-7

    Plasmid
    #57948
    Purpose
    Localization: Membrane/Vesicles, Excitation: 549, Emission: 565
    Depositor
    Michael Davidson
    Insert
    C-Src
    Use
    Tags
    mOrange2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
Showing: 81 - 100 of 234 results