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Plasmid#224212PurposeBacteroides genomic editing tool; IPTG inducible-Fncpf1DepositorInsertFncpf1
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFW5000
Plasmid#224214PurposeE. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin), tetQDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFW6000
Plasmid#224215PurposeBacteroides genomic editing tool; aTc inducible-Fncpf1DepositorInsertFncpf1
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
His SUMO TEV R221C SPOP 28-359
Plasmid#194116PurposeR221C His SUMO SPOP expression plasmidDepositorInsertSPOP R221C (SPOP Human)
UseTags6His-SUMO-TEVExpressionBacterialMutationR221CPromoterAvailable sinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHis8-4b-McDAH
Plasmid#140129PurposeExpresses McDAH with N-terminal 8xHis-tag in BL21 E. coliDepositorInsertDechloroacutumine halogenase from Menispermum canadense
UseTags8x-His, TEV protease site (ENLYFQ)ExpressionBacterialMutationPromoterT7Available sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
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GST-TPX2(1-43)
Plasmid#69107PurposeFor bacterial expression of human TPX2(1-43aa) tagged with GST.DepositorInsertTargeting Protein for Xklp2 (TPX2 Human)
UseTagsGSTExpressionBacterialMutationAmino acids 1-43 expressed.PromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
MBP-GTSE1-His6
Plasmid#69109PurposeFor bacterial expression of recombinant human GTSE1 tagged with MBP and His.DepositorInsertG2- and S-phase Expressed 1 (GTSE1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
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CBM9-(PT4)P
Plasmid#171708PurposeCarries CBM9 fusion tagDepositorInsertCellulose Bindind Protein 9
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFW2500
Plasmid#224213PurposeBacteroides genomic editing tool; IPTG inducible-Fncpf1 and recTDepositorInsertFncpf1 and recT
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pES E38A
Plasmid#13049DepositorInsertMutS
UseTagsHis6ExpressionBacterialMutationmutation Glu38 to AlaPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pETMutS-E41A
Plasmid#13048DepositorInsertMutS
UseTagsExpressionBacterialMutationmutation Glu41 to Ala full-lengthPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
ErbB-3-pGL3
Plasmid#60899Purposehuman ErbB-3 promoter reporter constructDepositorInsertErbB-3 promoter (ERBB3 Human)
UseLuciferaseTagsluciferaseExpressionMutationPromoterAvailable sinceNov. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCasTet-λ
Plasmid#128318PurposeConstitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.DepositorInsertTetR and pTetR/TetO
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationConstitutive expression of cas9 and anhydro-tetra…Promotertet promoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHS1528
Plasmid#205982PurposeU6-MWEE01000013 Cas5-HNH DR-BpiI-DRDepositorInsertCas5-HNH DR-BpiI-DR
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1184
Plasmid#205965PurposeNZ_CP025815 His14-TwinStrep-bdSUMO tagged DinG HNH protein (E. coli codon optimized) in pET45b(+)DepositorInsertDinG HNH
UseTags14xHis-TwinStrep-bdSUMOExpressionBacterialMutationPromoterT7Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1398
Plasmid#205971PurposeMWEE01000013 Cas5-HNH crRNA expression in pCOLADuet-1DepositorInsertCas5-HNH crRNA
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1182
Plasmid#205964PurposeNZ_CP025815 DinG HNH crRNA expression in pCOLADuet-1DepositorInsertDinG HNH crRNA
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only