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Plasmid#107695PurposeC-terminal half of π-EB1, mCherry-tagged, dissociates from microtubule ends in blue lightDepositorInsertMAPRE1 (MAPRE1 Human)
UseTagsZdk1 and mCherryExpressionMammalianMutationEB1 aa 186-268PromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB1N-LZ-mCherry-LOV2(wt)
Plasmid#107693PurposeN-terminal half of π-EB1 with wild-type LOV2, mCherry-tagged to track microtubule endsDepositorInsertMAPRE1 (MAPRE1 Human)
UseTagsA. sativa phototropin 1 LOV2 domain, GCN4 leucine…ExpressionMammalianMutationEB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…PromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5 HA GST Rheb1
Plasmid#14951DepositorInsertRheb1 (RHEB Human)
UseTagsHA-GSTExpressionMammalianMutationPromoterAvailable sinceMay 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEB1N-LZ-LOV2(fast)
Plasmid#107690PurposeN-terminal half of π-EB1 with fast LOV2, no fluorescent tagDepositorInsertMAPRE1 (MAPRE1 Human)
UseTagsA. sativa phototropin 1 LOV2 domain (I427V, H519L…ExpressionMammalianMutationEB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…PromoterCMVAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 EB1 shRNA #3
Plasmid#37927DepositorInsertEB1 shRNA #3 (MAPRE1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterhuman U6Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mVenus JBC
Plasmid#103987PurposePlasmid encoding mVenus JBCDepositorInsertmVenus JBC
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (F46L, F64L, …PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB1-2ndVal mGFPmut2
Plasmid#104033PurposePlasmid encoding 2ndVal mGFPmut2DepositorInsert2ndVal mGFPmut2
UseLow copyTagsExpressionBacterialMutationmGFPmut2 with valine inserted in 2nd positionPromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST/N1-hEB1-GFP
Plasmid#27382DepositorInsertmicrotubule-associated protein, RP/EB family, member 1 (MAPRE1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCB150-2_GFP-EB1
Plasmid#46364DepositorInsertEB1 (MAPRE1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterpBABEAvailable sinceJuly 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hRREB1-RFP
Plasmid#22924DepositorInserthuman ras responsive element binding protein 1 driving DsRedExpress (RREB1 Human)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 EB1 shRNA #1
Plasmid#37928DepositorInsertEB1 shRNA #1 (MAPRE1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterhuman U6Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
human EB1 in pBluescript II
Plasmid#39324DepositorInsertEB1 (MAPRE1 Human)
UseTagsExpressionBacterial and MammalianMutationPromoterT7Available sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEB1N-mApple-LZ-LOV2
Plasmid#197418PurposeMammalian expression of the mApple-tagged N-terminal half of photo-inactivated π-EB1DepositorInsertMAPRE1 (MAPRE1 Human)
UseTagsA. sativa phototropin 1 LOV2 domain, GCN4 leucine…ExpressionMammalianMutationEB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…PromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-π-EB1_IRES-mCherry
Plasmid#197427PurposeHomology-directed repair template targeting IRES-based π-element to MAPRE1 exon 5DepositorInsertIRES-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
UseCRISPR and Synthetic BiologyTagsGCN4 leucine zipper, LOV2, Zdk1, and mCherryExpressionMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-π-EB1_EF1α-mCherry
Plasmid#197429PurposeHomology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5DepositorInsertEF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
UseCRISPR and Synthetic BiologyTagsGCN4 leucine zipper, LOV2, Zdk1, and mCherryExpressionMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-π-EB1_EF1α-EGFP
Plasmid#197430PurposeHomology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5DepositorInsertEF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
UseCRISPR and Synthetic BiologyTagsEGFP, GCN4 leucine zipper, LOV2, and Zdk1ExpressionMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
Top7 Eb13 triple
Plasmid#12475DepositorInsertTop7 Eb13 triple
UseTags6xHisExpressionBacterialMutationEb13 triple. Top7 extended.PromoterAvailable sinceMarch 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
FUGW-d2GFP-ZEB1 3'UTR
Plasmid#79601PurposeFUGW lentiviral vector with constitutively expressed destabilized GFP (d2GFP) regulated by the human ZEB1 3'UTR. When used in combination with Ecad-RFP, comprises the Z-cad dual sensor.DepositorInsertd2GFP-Zeb1 3'UTR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 Flag Raptor Rheb15
Plasmid#26634DepositorInsertraptor-Rheb15 (RPTOR Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET22b-mEB1-eGFP-6xHis
Plasmid#130981PurposeBacterial expression of mouse EB1-GFP-6xHis for protein purification.DepositorInsertEB1-GFP-6xHis (Mapre1 Mouse)
UseTagseGFP-6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only