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Showing: 81 - 100 of 501 results

  1. pBig1a zz TEV YBBR POT1 MBP TEV TPP1 MBP TEV TIN2 ZZ TEV TRF2 (4comp2)

    Plasmid
    #185448
    Purpose
    Coexpresses human POT1 with a zz affinity tag, YBBR site, and TEV site, human TRF2 with a zz tag and TEV site, and human TIN2 and TPP1 each with an MBP affinity tag and TEV site in insect cells
    Depositor
    Ahmet Yildiz
    Article
    Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.
    Inserts
    TRF2 (TERF2 Human)
    POT1 (POT1 Human)
    TPP1 (TPP1 Human)
    TIN2 (TINF2 Human)
    Use
    Tags
    MBP, YBBR, and ZZ
    Expression
    Insect
    Mutation
    See Depositor Comments Below
    Promoter
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    185448-plasmid-map-sequence-id-364976

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  2. biGBac Kit

    Plasmid Kit
    #1000000088
    Purpose
    Generation of baculoviral transfer vectors coding up to 25 protein complex subunits in two Gibson assembly steps. Multisubunit protein complexes can be generated in baculovirus-infected insect cells.
    Depositor
    Jan-Michael Peters
    Available since
    Oct. 25, 2016
    Availability
    Academic Institutions and Nonprofits only

  3. UbiGate Components (set1)

    Plasmid Kit
    #1000000144
    Purpose
    Generate bacterial expression cassettes of up to six ORFs simultaneously; also contains ubiquitin, the E1 UBA1, an E2 collection, and a selection of E3s and their substrates for ubiquitination assays
    Depositor
    Marco Trujillo
    Available since
    Feb. 1, 2019
    Availability
    Academic Institutions and Nonprofits only

  4. UbiGate E2 activity screen (set2)

    Plasmid Kit
    #1000000145
    Purpose
    Contains bacterial constructs for E3 autoubiquitination assay, including expression cassettes containing ubiquitin and the E1 UBA1 in combination with all Arabidopsis thaliana E2s
    Depositor
    Marco Trujillo
    Available since
    Feb. 1, 2019
    Availability
    Academic Institutions and Nonprofits only

  5. psp-161-si1(sigfp)

    Plasmid
    #58801
    Purpose
    is a lentiviral vector coding the shRNA si1 (sigfp) and the gene of resistance to puromycin.
    Depositor
    Branka Horvat
    Insert
    si1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Human H1
    Available since
    Aug. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    58801-plasmid-map-sequence-id-91096

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  6. psp-161-si2(sip1090)

    Plasmid
    #58802
    Purpose
    is a lentiviral vector coding the shRNA si2 (sip1090) and the gene of resistance to puromycin.
    Depositor
    Branka Horvat
    Insert
    si2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    human H1
    Available since
    Aug. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    58802-plasmid-map-sequence-id-91098

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  7. p(+)MV-NSE-FlagP-M502-3p

    Plasmid
    #58748
    Purpose
    Expresses recombinant measles virus with Flag-P gene tagged si2 (siP) target sequence
    Depositor
    Branka Horvat
    Insert
    Measles virus antigenome
    Use
    Tags
    Flag
    Expression
    Mutation
    Promoter
    T7
    Available since
    Aug. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    58748-plasmid-map-sequence-id-90880

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  8. p(+)MV-NSE-FlagP-F497A-3p

    Plasmid
    #58796
    Purpose
    Expresses recombinant measles virus with Flag-P-F497A gene tagged si2 (siP) target sequence
    Depositor
    Branka Horvat
    Insert
    Measles virus antigenome
    Use
    Tags
    Flag
    Expression
    Mutation
    MeV-P F497A
    Promoter
    T7
    Available since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    58796-plasmid-map-sequence-id-91091

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  9. p(+)MV-NSE-FlagP-F497D-3p

    Plasmid
    #58797
    Purpose
    Expresses recombinant measles virus with Flag-P-F497D gene tagged si2 (siP) target sequence
    Depositor
    Branka Horvat
    Insert
    Measles virus antigenome
    Use
    Tags
    Flag
    Expression
    Mutation
    MeV-P F497D
    Promoter
    T7
    Available since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    58797-plasmid-map-sequence-id-91092

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  10. p(+)MV-NSE-FlagrP-3g_haP-M502-3p

    Plasmid
    #58798
    Purpose
    Expresses recombinant measles virus with duplicated P gene tagged with Flag and HA peptides and si1 (siGFP) and si2 (siP) target sequences, respectively.
    Depositor
    Branka Horvat
    Insert
    Measles virus antigenome
    Use
    Tags
    Flag and Ha
    Expression
    Mutation
    Promoter
    T7
    Available since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    58798-plasmid-map-sequence-id-91093

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  11. p(+)MV-NSE-FlagrP-3g_haP-F497A-3p

    Plasmid
    #58799
    Purpose
    Expresses recombinant measles virus with duplicated P gene. wt P gene and P-F497A gene are tagged with Flag and HA peptides and si1 (siGFP) and si2 (siP) target sequences, respectively.
    Depositor
    Branka Horvat
    Insert
    Measles virus antigenome
    Use
    Tags
    Flag and Ha
    Expression
    Mutation
    Promoter
    T7
    Available since
    Aug. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    58799-plasmid-map-sequence-id-91094

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  12. p(+)MV-NSE-FlagrP-3g_haP-F497D-3p

    Plasmid
    #58800
    Purpose
    Expresses recombinant measles virus with duplicated P gene. wt P gene and P-F497D gene are tagged with Flag and HA peptides and si1 (siGFP) and si2 (siP) target sequences, respectively.
    Depositor
    Branka Horvat
    Insert
    Measles virus antigenome
    Use
    Tags
    Flag and Ha
    Expression
    Mutation
    Promoter
    T7
    Available since
    Aug. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    58800-plasmid-map-sequence-id-91095

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  13. WDR5 (2GNQ)

    Plasmid
    #25489
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    WDR5 (WDR5 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. WDR5

    Plasmid
    #36900
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    WDR5 (WDR5 Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    mutation version of plasmid #60121 3UR4
    Promoter
    Available since
    Aug. 8, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. pLM1110_LEU2

    Plasmid
    #168460
    Purpose
    Big-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.
    Depositor
    Jef Boeke
    Article
    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118
    Insert
    mRFP1
    Use
    Synthetic Biology; Yeast assembly vector for big-…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    168460-plasmid-map-sequence-id-365349

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  20. pRB051_LEU2

    Plasmid
    #196993
    Purpose
    Big-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.
    Depositor
    Matthew Maurano
    Article
    Brosh et al Mol Cell. 2023 Apr 6;83(7):1140-1152.e7.
    Type
    Empty backbone
    Use
    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    196993-plasmid-map-sequence-id-394871

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Showing: 81 - 100 of 501 results