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Plasmid#181896PurposeFluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates, C-terminal deletionDepositorInsertnE-mHP1alpha-delCTE (Cbx5 Mouse)
UseTagsGFPExpressionMammalianMutationS11E, S12E, S13E, S14E, 14 aa C-terminal deletionPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-FUSN-mHP1alpha
Plasmid#181897PurposeFluorescently tagged HP1alpha fused to the N-terminal domain of the fused in sarcoma protein (FUSN)DepositorInsertFUSN-mHP1alpha (Cbx5 Mouse, Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-PGL-1-mHP1alpha
Plasmid#181899PurposeFluorescently tagged HP1alpha fused to PGL-1DepositorInsertPGL-1-mHP1alpha (Cbx5 Nematode, Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTub-alpha-1-mtGFP
Plasmid#138981PurposeNeuron-specific expression of membrane targeted EGFPDepositorInsertEGFP
UseTagsExpressionMammalianMutationEGFP (added membrane targeting domain from GAP43).PromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIRESneo-EGFP-alpha Tubulin
Plasmid#12298DepositorInsertEGFP-alpha Tubulin (TUBA1B Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
IRE1 alpha-pcDNA3.EGFP
Plasmid#13009DepositorInsertInositol Requiring Enzyme-1 (ERN1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-AtUBQ10:GFP-T35S
Plasmid#211836PurposePlant transient expression cassette for GFP (control) under UBQ10 promoter.DepositorInsertGFP control
UseSynthetic BiologyTagsGFPExpressionPlantMutationPromoterUBIQUITIN10Available sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT-EF-Pdgfralpha-EGFPN D842V
Plasmid#66789PurposeExpression of constitutively active murine PDGFRalpha D842V tagged with GFP under the control of EF1a promoterDepositorInsertPDGFRalpha (Pdgfra Mouse)
UseTagsEGFPExpressionMammalianMutationD842V (constitutively active)PromoterEF1aAvailable sinceJuly 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1_35S-eGFP-T35S (GB4279)
Plasmid#225434PurposeTranscriptional unit for constitutive expression of the enhanced GFP.DepositorInsert35S:eGFP:T35S
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
IRE1 alpha KA-pcDNA3.EGFP
Plasmid#13010DepositorInsertInositol Requiring Enzyme-1 (ERN1 Human)
UseTagsExpressionMammalianMutationR4L, K599APromoterCMVAvailable sinceNov. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pIREShyg-PA-GFP-alpha Tubulin
Plasmid#12296DepositorInsertalpha Tubulin (TUBA1B Human)
UseTagsPA-GFPExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pIRESneo-PA-GFP-alpha Tubulin
Plasmid#12297DepositorInsertalpha Tubulin (TUBA1B Human)
UseTagsPA-GFPExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTub-alpha1-Lifeact-GFP
Plasmid#175437PurposeF-actin structures labellingDepositorInsertLifeact-EGFP
UseTagsEGFPExpressionMammalianMutationPromoterpTub-alpha1Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEBB-II-I-GFP alpha isoform
Plasmid#22146DepositorInsertTFII-I (GTF2I Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(+)
Plasmid#184334PurposeExpression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.DepositorInsertNrxn3-alpha (Nrxn3 Mouse)
UseTagsHA-tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1alpha-soCoChR-GFP
Plasmid#107709PurposeA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoterDepositorInsertsoCoChR-GFP
UseAAVTagsEGFP and KA2ExpressionMammalianMutationPromoterEF1alphaAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-nAChr-Alpha6-GFP
Plasmid#50486Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChr-alpha6 (Chrna6 Mouse)
UseTagsGFP fusion in M3-M4 loop (after residue A405)ExpressionMammalianMutationGly-Ala-Gly flexible linker flanking the GFP open…PromoterCMVAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNL-EGFP/EF1alpha/WPREdU3
Plasmid#18698DepositorInsertEGFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRGG-GFP-RGG-mHP1alpha
Plasmid#181898PurposeFluorescently tagged HP1alpha fused to the arginine/glycine-rich RGG domain of LAF-1DepositorInsertRGG-GFP-RGG (Cbx5 Nematode, Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS
Plasmid#71396Purpose2Gi2R assayDepositorInserts2xGFP:NES
2xRFP:NLS
UseLentiviralTagsIRESExpressionMammalianMutationPromoterEF1alpha and IRESAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only