-
Plasmid#108509Purposeexpression of vsv-INCENP 1-63-EGFPDepositorInsertCEN-Box of INCENP (INCENP Human)
UseTagsEGFP and VSVExpressionMammalianMutationonly CEN box of INCENPPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-vsv-Mis12-dCEN-Incenp-EGFP
Plasmid#108478Purposeexpression of vsv-Mis12-dCEN-INCENP-EGFPDepositorInsertMis12-dCEN-INCENP (INCENP Human)
UseTagsEGFP and VSVExpressionMammalianMutationCEN box replaced by Mis12PromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLPCX2-HA-GATA3 N1(1-259)
Plasmid#89830PurposeExpress GATA3 N1 in mammalian cellsDepositorInsertGata3 (Gata3 Mouse)
UseRetroviralTagsHAExpressionMutationN1 (AA 1-259)PromoterCMVAvailable sinceMay 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLPCX2-HA-GATA3 C1(295-443)
Plasmid#90316PurposeExpress GATA3 C2 in mammalian cellsDepositorInsertGATA3 (Gata3 Mouse)
UseRetroviralTagsHAExpressionMutationC2 (AA 295-443)PromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLPCX2-HA-GATA3 N2(1-294)
Plasmid#89831PurposeExpress GATA3 N2 in mammalian cellsDepositorInsertGata3 (Gata3 Mouse)
UseRetroviralTagsHAExpressionMutationN2 (AA 1-294)PromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO shMKP7.v2 puro
Plasmid#87795PurposeExpresses an inducible short hairpin targeting human MKP7 sequenceDepositorInsertshMKP7 (DUSP16 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE tightAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmiRc2 3XFLAG-MKK4-EE-NLS
Plasmid#87776PurposeUsed as a donor vector containing N-term 3xFLAG to clone into pSLIK-HygroDepositorInsertMKK4 (MAP2K4 Human)
UseTags3xFLAG and NLSExpressionMutationPromoterAvailable sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSicoR (EGFP) shPnky-2
Plasmid#79142PurposeStable expression of shRNA targeting mouse Pnky. The shRNA (and EGFP) can be excised by the addition of CreDepositorInsertshPnky-2 (Gm30731 Mouse)
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterU6 for the shRNA and CMV for EGFPAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)mGAT1-565-YFP-566CT
Plasmid#41677DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)
UseTagsYFPExpressionMammalianMutation“Monomerizing” YFP A206K mutation; YFP inserted d…PromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)mGAT1-570-CFP-571CT
Plasmid#41680DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)
UseTagsCFPExpressionMammalianMutation“Monomerizing” CFP A206K mutation; CFP inserted d…PromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)mGAT1-570-YFP-571CT
Plasmid#41679DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)
UseTagsYFPExpressionMammalianMutation“Monomerizing” YFP A206K mutation; YFP inserted d…PromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)mGAT1-565-CFP-566CT
Plasmid#41678DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)
UseTagsCFPExpressionMammalianMutation“Monomerizing” CFP A206K mutation; CFP inserted d…PromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd6_4-330/pET28b(+) N-tag
Plasmid#71351Purposecsd6, construct:4-330, pET28b(+) N-tagDepositorInsertHP0518 (HP0518 )
UseTagsHistidine tagExpressionBacterialMutationPromoterT7Available sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd6_13-330/pET28b(+) N-tag
Plasmid#71352Purposecsd6, construct:13-330, pET28b(+) N-tagDepositorInsertHP0518 (HP0518 )
UseTagsHistidine tagExpressionBacterialMutationPromoterT7Available sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd6_1-330/pET28b(+) N-tag
Plasmid#71350Purposecsd6, construct:1-330, pET28b(+) N-tagDepositorInsertHP0518 (HP0518 )
UseTagsHistidine tagExpressionBacterialMutationPromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALentry
Plasmid#69233PurposeR1335K mutant SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)DepositorInsertSpCas9
UseCRISPRTags2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…ExpressionMammalianMutationArginine 1335 is changed to LysinePromoterAvailable sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
1.2 hA33 luciferase
Plasmid#68149Purposeluciferase reporter for hA33 promoterDepositorInsertA33 (GPA33 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationstarts at -1149 relative to ATGPromoterhuman GPA33 promoterAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
0.6 hA33 luciferase
Plasmid#68150Purposeluciferase reporter for hA33 promoterDepositorInsertA33 (GPA33 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationstarts at -595 relative to ATGPromoterhuman GPA33 promoterAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
0.44 hA33 luciferase
Plasmid#68151Purposeluciferase reporter for hA33 promoterDepositorInsertA33 (GPA33 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationstarts at -443 relative to ATGPromoterhuman GPA33 promoterAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
0.42 hA33 luciferase
Plasmid#68152Purposeluciferase reporter for hA33 promoterDepositorInsertA33 (GPA33 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationstarts at -423 relative to ATGPromoterhuman GPA33 promoterAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only