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Addgene
Showing: 9941 - 9960 of 16559 results

  1. DsRed-CPI-17-T38E-NLS

    Plasmid
    #100790
    Purpose
    Mammalian expression of Fluorescent CPI-17 phospho mimetic nuclear
    Depositor
    Joseph Gordon
    Insert
    CPI-17-T38E-NLS (PPP1R14A Human)
    Use
    Tags
    DsRed
    Expression
    Mammalian
    Mutation
    T38E
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    100790-plasmid-map-sequence-id-193221

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  2. pOpen-T7gene5

    Plasmid
    #165534
    Purpose
    DNA Pol with high fidelity and rapid extension rate (useful in copying long stretches of DNA); Has strong 3'-5' exonuclease.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    T7 DNA Polymerase (unmodified)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    165534-plasmid-map-sequence-id-339124

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  3. pLKO.3G_X7

    Plasmid
    #171213
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses eGFP for selection. Please see cloning information for suggested restriction sites
    Depositor
    Jenny Wang
    Insert
    N/A; empty backbone
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    171213-plasmid-map-sequence-id-338282

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  4. pBac[3xP3-gTc'v;Tc'puP-Tc'H2Av_EGFP]

    Plasmid
    #86455
    Purpose
    Nuclear reporter (histone-tagged EGFP) construct with Tribolium specific polyubiquitin promoter (driving ubiquitous expression); (Transformation plasmid; Black eye marker)
    Depositor
    Gregor Bucher
    Insert
    3xP3-gTc'v;Tc'puP-Tc'H2Av_EGFP
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    86455-plasmid-map-sequence-id-171155

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  5. pCRII-Topo Hnrp

    Plasmid
    #45872
    Depositor
    Jeffrey Macklis
    Insert
    Hnrnpa2b1 in situ probe (Hnrpa2b1 Mouse)
    Use
    In situ
    Tags
    Expression
    Mutation
    contains bp# 1145-1370 of NM_182650.3
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  6. SIRT6

    Plasmid
    #28271
    Depositor
    Cheryl Arrowsmith
    Insert
    SIRT6 (SIRT6 Human)
    Use
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. PP-HSP90 (3Q5J)

    Plasmid
    #28281
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-HSP90
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    N-terminal domain
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. PP-G6PI

    Plasmid
    #28283
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-G6PI (PF14_0341 Plasmodium falciparum)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    28283-plasmid-map-sequence-id-235428

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  9. mEmerald-Nesprin3-C-18

    Plasmid
    #54203
    Purpose
    Localization: Nuclear Membrane , Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Nesprin3 (SYNE3 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    54203-plasmid-map-sequence-id-247990

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  10. mCherry-NUP50-N-10

    Plasmid
    #55111
    Purpose
    Localization: Nuclear Pore, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    NUP50 (NUP50 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only

  11. pX330-Flag-HFSpCas9-H840A

    Plasmid
    #80455
    Purpose
    high fidelity SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Insert
    HFSpCas9_H840A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Availability
    Academic Institutions and Nonprofits only
    80455-plasmid-map-sequence-id-151476

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  12. pExp-mCherry

    Plasmid
    #112569
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and mCherry (mCherry fluorescent protein,) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-mCherry and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112569-plasmid-map-sequence-id-217213

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  13. pExp-TF

    Plasmid
    #112575
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-TriggerFactor and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112575-plasmid-map-sequence-id-217211

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  14. p3E_he1a:ECFP

    Plasmid
    #113880
    Purpose
    p3E entry vector for Three Multisite Gateway
    Depositor
    Marnie Halpern
    Insert
    he1a (he1.1 Zebrafish)
    Use
    Zebrafish danio expression
    Tags
    ECFP
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    113880-plasmid-map-sequence-id-222569

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  15. P12m

    Plasmid
    #120938
    Purpose
    MoClo golden gate assembly AB part for pLas-TetO (Promoter activated by LasR and repressed by TetR; encodes <3OC12 AHL AND aTc> logic). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ohAB carb pLasTetO
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120938-plasmid-map-sequence-id-256615

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  16. P15m

    Plasmid
    #120939
    Purpose
    MoClo golden gate assembly AB part for pCin-TetO (Promoter activated by CinR and repressed by TetR; encodes <3OC14 AHL AND aTc> logic). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ohAB carb pCinTetO
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120939-plasmid-map-sequence-id-238035

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  17. P16m

    Plasmid
    #120940
    Purpose
    MoClo golden gate assembly AB part for pCin-LacO (Promoter activated by CinR and repressed by LacI; encodes <3OC14 AHL AND IPTG> logic). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ohAB carb pCinLacO m
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120940-plasmid-map-sequence-id-238036

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  18. P32m

    Plasmid
    #120943
    Purpose
    MoClo golden gate assembly AB part for PlacO (common LacI-repressed promoter; see DOI: 10.1126/science.1256272). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    PlacO (Tim Lu, Matt Bennett, etc)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120943-plasmid-map-sequence-id-236887

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  19. P33m

    Plasmid
    #120944
    Purpose
    MoClo golden gate assembly AB part (medium-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    P11 from Mutalik library (medium)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120944-plasmid-map-sequence-id-245421

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  20. P34m

    Plasmid
    #120945
    Purpose
    MoClo golden gate assembly AB part (low-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    P13 from Mutalik library (weak)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120945-plasmid-map-sequence-id-238039

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Showing: 9941 - 9960 of 16559 results