Addgene

We narrowed to 16,105 results for: cel.2

Showing: 9901 - 9920 of 16105 results

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pB-6HIS-TEV-mEGFP,IRES-NGFR

Plasmid

#164521

Purpose

All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal His-TEV-GFP tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)

Depositor

Mitchell Guttman

Article

Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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LIC4B-His-TEV-TAK1-TAB1

Plasmid

#154874

Purpose

Insect cell optimized TAK1-TAB1 construct used to solve structure and deposit PDB ID: 4O91

Depositor

Kenneth Westover

Article

Tan et al J Med Chem. 2015 Jan 8;58(1):183-96. doi

Insert

TAK1-TAB1 (TAB1 Human)

Use

Tags

LIC TEV His

Expression

Insect

Mutation

Codon optimized

Promoter

polyhedrin

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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NFATc1_L (OZ543)

Plasmid

#28068

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting NFATc1 (nfatc1 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
NFATc1_R (OZ544)

Plasmid

#28069

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting NFATc1 (nfatc1 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
pACAGW-ChR2-Venus-AAV

Plasmid

#20071

Purpose

AAV-mediated expression of ChR2-Venus for neuronal excitation

Depositor

Karel Svoboda

Article

Leopoldo et al Nature. 2009 Jan 18.

Has Service

AAV1 and AAV9

Insert

Channelrhodopsin-2 (CHLRE_02g085257v5 Chlamydomonas reinhardtii)

Use

AAV

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CAGG

Available since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pmiRA1-MYC

Plasmid

#141121

Purpose

Lentiviral overexpression of MYC in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

MYC (MYC Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Htt171-66Q-myc-WPRE

Plasmid

#107934

Purpose

Adeno-Associated viral (AAV) vector plasmid expressing exon1 of mutant HTT (mHTT, 66 polyQ repeats) in neurons

Depositor

Johan Jakobsson

Article

Pircs et al Cell Rep. 2018 Aug 7;24(6):1397-1406. do

Insert

Exon 1 of mutant HTT, 66 polyQ repeats (HTT Human)

Use

AAV

Tags

Expression

Mutation

Promoter

hSynapsin

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Htt171-18Q-myc-WPRE

Plasmid

#107936

Purpose

Adeno-Associated viral (AAV) vector plasmid expressing exon1 of wild type HTT (wtHTT, 18 polyQ repeats) in neurons

Depositor

Johan Jakobsson

Article

Pircs et al Cell Rep. 2018 Aug 7;24(6):1397-1406. do

Insert

Exon 1 of wild type HTT (HTT Human)

Use

AAV

Tags

Expression

Mutation

Promoter

hSynapsin

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-JAK1

Plasmid

#174572

Purpose

Human FLAG-tagged JAK1 expression (N-term. tag) (CMV prom.)

Depositor

Mathew Garnett

Article

Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

Insert

JAK1 (JAK1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_DA1m

Plasmid

#113049

Purpose

express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1m in neurons

Depositor

Yulong Li

Article

Sun et al Cell. 2018 Jul 12;174(2):481-496.e19. do

Has Service

AAV9

Insert

GPCR activation based DA sensor GRAB_DA1m (DRD2 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_DA1h

Plasmid

#113050

Purpose

express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h in neurons

Depositor

Yulong Li

Article

Sun et al Cell. 2018 Jul 12;174(2):481-496.e19. do

Has Service

AAV Retrograde and AAV9

Insert

GPCR activation based DA sensor GRAB_DA1h (DRD2 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A, E1707A) pcDNA3

Plasmid

#206121

Purpose

expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domains I, II, III and IV to to abolish divalent cation binding

Depositor

Annette Dolphin

Article

Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

Insert

cacna1a (Cacna1a Rat)

Use

Tags

Expression

Mammalian

Mutation

E320A, E670A, E1411A, E1707A

Promoter

CMV

Available since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGFP-nE-mHP1alpha

Plasmid

#181895

Purpose

Fluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

nE-mHP1alpha (Cbx5 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

S11E, S12E, S13E, S14E

Promoter

CMV

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-eSpCas9-2A-GFP-sgRNA-RDH11

Plasmid

#161924

Purpose

To generate RDH11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against RDH11 exon 2.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

sgRNA targeting RDH11 exon 2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-nE-mHP1alpha-delCTE

Plasmid

#181896

Purpose

Fluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates, C-terminal deletion

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

nE-mHP1alpha-delCTE (Cbx5 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

S11E, S12E, S13E, S14E, 14 aa C-terminal deletion

Promoter

CMV

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-FUSN-mHP1alpha

Plasmid

#181897

Purpose

Fluorescently tagged HP1alpha fused to the N-terminal domain of the fused in sarcoma protein (FUSN)

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

FUSN-mHP1alpha (Cbx5 Mouse, Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-PGL-1-mHP1alpha

Plasmid

#181899

Purpose

Fluorescently tagged HP1alpha fused to PGL-1

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

PGL-1-mHP1alpha (Cbx5 Mouse, Nematode)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pRGG-GFP-RGG-mHP1alpha

Plasmid

#181898

Purpose

Fluorescently tagged HP1alpha fused to the arginine/glycine-rich RGG domain of LAF-1

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

RGG-GFP-RGG (Cbx5 Mouse, Nematode)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCSC-CMV-HA-Ast-3-NP-1-IRES-mCherry

Plasmid

#159631

Purpose

expresses HA Allatostatin-3, Neurophysin-1, IRES mCherry under the CSC promoter

Depositor

Andrew Allen, Ross Bathgate

Article

Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

Insert

Ast (AstA Fly)

Use

Lentiviral

Tags

HA, IRES, mCherry, and neurophysin-1

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-Sox2-IP

Plasmid

#13459

Depositor

Shinya Yamanaka

Article

Maruyama et al J Biol Chem. 2005 Jul 1. 280(26):24371-9

Insert

Sox2 (Sox2 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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