Addgene

282,721 results

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NPC1 His6 EGFP

Plasmid

#53521

Depositor

Matthew Scott

Article

Lopez et al J Neurosci. 2011 Mar 23;31(12):4367-78.

Insert

NPC1

Use

Tags

EGFP and His6

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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NPC1 His6 EGFP

Plasmid

#53521

Depositor

Matthew Scott

Article

Lopez et al J Neurosci. 2011 Mar 23;31(12):4367-78.

Insert

NPC1

Use

Tags

EGFP and His6

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
hE-cadherin-pcDNA3

Plasmid

#45769

Depositor

Barry Gumbiner

Article

Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

Insert

hE-cadherin (CDH1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 24, 2013

Availability

Academic Institutions and Nonprofits only

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hE-cadherin-pcDNA3

Plasmid

#45769

Depositor

Barry Gumbiner

Article

Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

Insert

hE-cadherin (CDH1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 24, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAX-SCLM

Plasmid

#216757

Purpose

For strong and long-lasting expression of SuperClomeleon in mammalian cells from the hybrid CAG promoter

Depositor

George Augustine

Insert

SuperClomeleon

Use

Tags

Expression

Mammalian

Mutation

S30R and 11 AA deletion in the Cerulean CFP moiet…

Promoter

CAG (cytomegalovirus-chicken beta-actin-rabbit be…

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-eYFP

Plasmid

#104055

Purpose

An AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG promoter

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Has Service

AAV PHP.eB, AAV Retrograde, AAV2, and AAV5

Insert

EYFP

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-eYFP

Plasmid

#104055

Purpose

An AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG promoter

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Has Service

AAV PHP.eB, AAV Retrograde, AAV2, and AAV5

Insert

EYFP

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1a-mitoGFP-DIO

Plasmid

#174112

Purpose

Cre-dependent expression of mitochondria-targeted Green Fluorescent Protein under control of the EF1a promoter

Depositor

Maarten Kole

Article

Kole et al Nat Commun. 2022 Dec 9;13(1):7598. doi:

Insert

mitoGFP (COX8A Human)

Use

AAV

Tags

Cox8 targeting sequence and GFP

Expression

Mutation

Promoter

Ef1a

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1a-mitoGFP-DIO

Plasmid

#174112

Purpose

Cre-dependent expression of mitochondria-targeted Green Fluorescent Protein under control of the EF1a promoter

Depositor

Maarten Kole

Article

Kole et al Nat Commun. 2022 Dec 9;13(1):7598. doi:

Insert

mitoGFP (COX8A Human)

Use

AAV

Tags

Cox8 targeting sequence and GFP

Expression

Mutation

Promoter

Ef1a

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-hALIX

Plasmid

#21504

Depositor

James Hurley

Article

Lee et al Science. 2008 Oct 24. 322(5901):576-80.

Insert

ALIX (PDCD6IP Human)

Use

Tags

Cherry

Expression

Bacterial

Mutation

none

Promoter

Available since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only

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mCherry-hALIX

Plasmid

#21504

Depositor

James Hurley

Article

Lee et al Science. 2008 Oct 24. 322(5901):576-80.

Insert

ALIX (PDCD6IP Human)

Use

Tags

Cherry

Expression

Bacterial

Mutation

none

Promoter

Available since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
DsRed-rab11 DN

Plasmid

#12680

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB11A (Homo sapiens) (RAB11A Human)

Use

Tags

DsRed and HA

Expression

Mammalian

Mutation

changed serine 25 to asparagine

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
DsRed-rab11 DN

Plasmid

#12680

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB11A (Homo sapiens) (RAB11A Human)

Use

Tags

DsRed and HA

Expression

Mammalian

Mutation

changed serine 25 to asparagine

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
HA.PKD

Plasmid

#10808

Depositor

Alex Toker

Article

Storz et al EMBO J. 2003 Jan 2. 22(1):109-20.

Insert

PKD (PRKD1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

L520P

Promoter

CMV

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
HA.PKD

Plasmid

#10808

Depositor

Alex Toker

Article

Storz et al EMBO J. 2003 Jan 2. 22(1):109-20.

Insert

PKD (PRKD1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

L520P

Promoter

CMV

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS_4xMma PylT_FLAG-AbKRS-chIPYE

Plasmid

#140020

Purpose

Plasmid with 4xMmaPylT cassette and chimeric Mma/Mba PylRS for amber suppression and incorporation of photo-crosslinking-lysine; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

AbKRS-chIPYE (pylS Methanosarcina mazei and Methanosarcina bakeri)

Use

Tags

FLAG

Expression

Mammalian

Mutation

1-149 MbPylRS IPYE (V31I, T56P, H62Y, A100E) + 18…

Promoter

EF1

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT_FLAG-AbKRS-chIPYE

Plasmid

#140020

Purpose

Plasmid with 4xMmaPylT cassette and chimeric Mma/Mba PylRS for amber suppression and incorporation of photo-crosslinking-lysine; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

AbKRS-chIPYE (pylS Methanosarcina mazei and Methanosarcina bakeri)

Use

Tags

FLAG

Expression

Mammalian

Mutation

1-149 MbPylRS IPYE (V31I, T56P, H62Y, A100E) + 18…

Promoter

EF1

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pTKRED

Plasmid

#41062

Depositor

Edward Cox, Thomas Kuhlman

Article

Kuhlman et al Nucleic Acids Res. 2010 Apr;38(6):e92. E

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pTKRED

Plasmid

#41062

Depositor

Thomas Kuhlman, Edward Cox

Article

Kuhlman et al Nucleic Acids Res. 2010 Apr;38(6):e92. E

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.hSynapsin1.OxLight1

Plasmid

#169792

Purpose

Fluorescent reporter for orexin neuropeptides

Depositor

Tommaso Patriarchi

Article

Duffet et al Nat Methods. 2022 Feb;19(2):231-241. doi

Insert

OxLight1

Use

AAV

Tags

Expression

Mutation

Promoter

human Synapsin-1

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge