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Plasmid#178775PurposeGolden Gate GFP dropout vector to generate S. thermophilus type I-E single arraysDepositorInsertgfp dropout to generate S. thermophilus type I-E single arrays
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEh_gRNA1
Plasmid#178758PurposeExpression of a gRNA that targets next to PAM library, used for E. haloalkaliphila type I-E systemDepositorInsertgRNA that targets next to PAM library, used for E. haloalkaliphila type I-E system
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMs_gRNA1
Plasmid#178764PurposeExpression of a gRNA that targets next to PAM library, used for Marinomonas sp. type I-E systemDepositorInsertgRNA that targets next to PAM library, used for Marinomonas sp. type I-E system
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Xalb_I-C_array_GG
Plasmid#178767PurposeGolden Gate vector to generate X. albilineans type I-C single arraysDepositorInsertGolden Gate vector BaeI recognition site to generate X. albilineans type I-C single arrays
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc3_gRNA1
Plasmid#178746PurposeExpression of a gRNA that targets next to PAM library, used for A. chroococcum type I-E #3 systemDepositorInsertgRNA that targets next to PAM library, used for A. chroococcum type I-E #3 system
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLm_gRNA1
Plasmid#178752PurposeExpression of a gRNA that targets next to PAM library, used for L. mobilis type I-E systemDepositorInserta gRNA that targets next to PAM library, used for L. mobilis type I-E system
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc2_gRNA1
Plasmid#178740PurposeExpression of a gRNA that targets next to PAM library, used for A. chroococcum type I-E #2 systemDepositorInsertgRNA that targets next to PAM library, used for A. chroococcum type I-E #2 system
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH3137
Plasmid#179617PurposePunc-4::GCaMP6::RFP unc-54 3' UTR C.elegans A-MNs and others expression of GCaMP6::RFPDepositorInsertGCaMP6
UseTagsRFPExpressionWormMutationPromoterPunc-4Available sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-CAAAG
Plasmid#170096PurposedeGFP reporter plasmid with CAAAG PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterp70aAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-CAATG
Plasmid#170097PurposedeGFP reporter plasmid with CAATG PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterp70aAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-GTAAT
Plasmid#170098PurposedeGFP reporter plasmid with GTAAT PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterp70aAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-GTATT
Plasmid#170099PurposedeGFP reporter plasmid with GTATT PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterp70aAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-PacI
Plasmid#170100PurposedeGFP reporter plasmid with unique PacI restriction enzyme recognition siteDepositorInsertdeGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterp70aAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEc-crRNA2
Plasmid#170089Purposeencodes E. coli type I-E single spacer arrayDepositorInsertE. coli type I-E single spacer array
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterJ23119Available sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-ATAAC
Plasmid#170095PurposedeGFP reporter plasmid with ATAAC PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterp70aAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMPRMA43
Plasmid#172944PurposepQE30 encoding Wzz mutant i191DepositorInsertShigella flexneri wzz i191 mutant gene
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWSK29-wzzBST
Plasmid#172968PurposepWSK29 with Salmonella typhimurium wzzBST geneDepositorInsertwzzB gene from Salmonella enterica serovar Typhimurium
UseTagsExpressionBacterialMutationK198E- please see depositor commentsPromoterAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMPRMA47
Plasmid#172947PurposepQE30 encoding Wzz mutant i255DepositorInsertShigella flexneri wzz i255 mutant gene
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWSK29-fepEST
Plasmid#172969PurposepWSK29 with Salmonella typhimurium fepEST geneDepositorInsertfepE gene from Salmonella enterica serovar Typhimurium
UseTagsExpressionBacterialMutationG160S- please see depositor commentsPromoterAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMPRMA49
Plasmid#172948PurposepQE30 encoding Wzz mutant i81DepositorInsertShigella flexneri wzz i81 mutant gene
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only