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Plasmid#75024PurposeCan be used to drive expression in zebrafish astrocytes.DepositorInsertglial fibrillary acidic protein promoter (gfap Zebrafish)
Use5' entry vector for multisite gateway three …TagsExpressionMutationPromotergfapAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-gRNA hUbC-VP64-dSaCas9-VP64-T2A-Thy1.1
Plasmid#194279PurposeExpresses gRNA and VP64-dSaCas9-VP64 from lentiviral vectorDepositorInsertshumanized VP64 dSaCas9 VP64 T2A Thy1.1
gRNA
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhU6 and hUbCAvailable sinceFeb. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K11R
Plasmid#121154PurposeMammalian expression of HA tagged ubiquitin K11RDepositorInsertUbiquitin C (UBC Human)
UseTagsExpressionMammalianMutationK11 mutated to an RPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K6
Plasmid#121151PurposeMammalian expression of HA tagged ubiquitin with K6 only, other lysines mutated to argininesDepositorInsertUbiquitin C (UBC Human)
UseTagsExpressionMammalianMutationAll Ks apart from K6 mutated to RsPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-ATOH1-T2A-PuroR
Plasmid#162342PurposeLentiviral expression of ATOH1 under the control of the TetON promoterDepositorInsertATOH1 (ATOH1 Human)
UseLentiviralTagsT2A-PuroRExpressionMammalianMutationPromoterTet-ONAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K27R
Plasmid#121155PurposeMammalian expression of HA tagged ubiquitin K27RDepositorInsertUbiquitin C (UBC Human)
UseTagsExpressionMammalianMutationK27 mutated to an RPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDEF3‐hTIM3 (no tag)
Plasmid#49212Purposeexpresses human Tim3DepositorInsertTim3 (HAVCR2 Human)
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-TRABID (OTU+AnkUBD, aa 245-697)
Plasmid#61590PurposeExpresses human TRABID (OTU+AnkUBD domains) in E. coli.DepositorInsertTRABID (ZRANB1 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-244 and aa 698-708.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
single polypeptide FPX biosensor for calcium ion
Plasmid#60887PurposeFPX biosensorDepositorInsertsingle polypeptide FPX biosensor for calcium ion
UseTagsHindIII siteExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD1 (full-length, aa 1-481)
Plasmid#61405PurposeExpresses human OTUD1 (full-length) in E. coli.DepositorInsertOTUD1 (OTUD1 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationPromoterT7Available sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
RANLS
Plasmid#50843PurposeExpresses one ddFP in mammalian cells, the second plasmid for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with plasmid GANES-DEVD-BNLS.DepositorInsertddRFP A
UseTagstriplicated NLS sequence DPKKKRKVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-erExRai-AKAR2
Plasmid#161756PurposeExRai-AKAR2 targeted to the cytosolic face of the ER membrane.DepositorInsertCYP450-ExRai-AKAR2
UseTagsN-terminal 27 amino acids from CYP450ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD3 (OTU, aa 52-209)
Plasmid#61412PurposeExpresses human OTUD3 (OTU domain) in E. coli.DepositorInsertOTUD3 (OTUD3 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-51 and aa 210-398.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-ExRai-AKAR2(T/A)
Plasmid#161754PurposeNegative-control mutant for ExRai-AKAR2 biosensor.DepositorInsertExRai-AKAR2(T/A)
UseTagsExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTol2p2A--3mnx1:loxP-mTagBFP2-stop-loxP-mCherry-NS-MCS
Plasmid#78251PurposeCan be used to subclone in tagged gene of interest into inducible motor neuron expression vector.DepositorInsertsUseZebrafish motor neuron inducible expressionTagsExpressionMutationPromoter-3mnx1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
BNLS
Plasmid#50836PurposeExpresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES).DepositorInsertddGFP B
UseTagstriplicated NLS sequence DPKKKRKVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K6R
Plasmid#121153PurposeMammalian expression of HA tagged ubiquitin K6RDepositorInsertUbiquitin C (UBC Human)
UseTagsExpressionMammalianMutationK6 mutated to an RPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD2 (full-length, aa 1-348)
Plasmid#61409PurposeExpresses human OTUD2 (full-length) in E. coli.DepositorInsertOTUD2 (YOD1 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationPromoterT7Available sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-Cezanne (OTU, aa 53-446)
Plasmid#61581PurposeExpresses human Cezanne (OTU domain) in E. coli.DepositorInsertCezanne (OTUD7B Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-52 and aa 447-843.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
GANLS
Plasmid#50852PurposeExpresses one ddFP in mammalian cells, the second plasmid for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with plasmid NESRA-DEVD-BNLS.DepositorInsertddGFP A
UseTagstriplicated NLS sequence DPKKKRKVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only