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Plasmid#83462PurposeCytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (502)DepositorInsertPolycystin-1 (PKD1 Human)
UseTagsCD16.7ExpressionMammalianMutationAmino-acids 4078-4303, W4107APromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO CD16.7-h-PKD1 FLM S4144D
Plasmid#83460PurposeCytoplasmic tail of human PC1 expression cassette with a membrane anchor and a point mutant that disrupts the second calmodulin binding domain (513)DepositorInsertPolycystin-1 (PKD1 Human)
UseTagsCD16.7ExpressionMammalianMutationAmino-acids 4078-4303, S4144DPromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO CD16.7-h-PKD1 FLM (4078-4169)
Plasmid#83463PurposeCytoplasmic tail of human PC1 expression cassette with a membrane anchor and PKD1 sequence that corresponds to 4078 to 4169 of PC1 (96)DepositorInsertPolycystin-1 (PKD1 Human)
UseTagsCD16.7ExpressionMammalianMutationAmino-acids 4078-4169PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO h-PKD1 FLS S4144D-Myc/His
Plasmid#83459PurposeHuman PC1-CTp30 expression cassette with a point mutant that disrupts the second calmodulin binding domain (514)DepositorInsertPolycystin-1 (PKD1 Human)
UseTags6xHis and MycExpressionMammalianMutationAmino-acids 4107-4303, S4144DPromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO h-PKD1 FLS (4127-4303)-Myc/His
Plasmid#83457PurposeHuman PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) (632)DepositorInsertPolycystin-1 (PKD1 Human)
UseTags6xHis and MycExpressionMammalianMutationAmino-acids 4127-4303PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO h-PKD1 FLS L4137P-GST
Plasmid#83455PurposeHuman PC1-CTp30 expression cassette with a patient mutation at L4137 and a GST-tag (848)DepositorInsertPolycystin-1 (PKD1 Human)
UseTagsGSTExpressionMammalianMutationAmino-acids 4107-4303, L4137PPromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO h-PKD1 FLS R4150C-GST
Plasmid#83454PurposeHuman PC1-CTp30 expression cassette with a patient mutation at R4150 and a GST-tag (850)DepositorInsertPolycystin-1 (PKD1 Human)
UseTagsGSTExpressionMammalianMutationAmino-acids 4107-4303, R4150CPromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Actin-7
Plasmid#57570PurposeLocalization: Actin, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertActin (ACTB Human)
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
tdEos-Zyxin-6
Plasmid#57696PurposeLocalization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertZyxin (ZYX Human)
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mKO-Vinculin-23
Plasmid#57852PurposeLocalization: Focal Adhesions, Excitation: 548, Emission: 561DepositorInsertVinculin (VCL Human)
UseTagsmKOExpressionMammalianMutationPromoterCMVAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASp-Flag-Myc-Qin-Tudor-attB
Plasmid#44210DepositorInsertQin-Tudor-domain (qin Fly)
UseFly transformationTagsFlag and MycExpressionMutationlacks E3 domain on N-term (del aa1-274)PromoterAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn NS34a-msGFP-A'Q-mito
Plasmid#158766PurposeLentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
UseLentiviralTagsExpressionMutationFlexible linkers between the protease and msGFP a…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn NS34a-msGFP-'Q-mito
Plasmid#158767PurposeLentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
UseLentiviralTagsExpressionMutationFlexible linkers between the protease and msGFP a…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYT7alpha-HaloTag TMD Cys-Ala
Plasmid#179716PurposeLentiviral expression of mouse Syt7 palmitoylation site mutant with C terminal HaloTagDepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pT7-hMLH1dn for IVT
Plasmid#178114PurposeTemplate for in vitro transcription of human MLH1dnDepositorInserthuman MLH1dn (codon optimized) (MLH1 Human)
UseTemplate for in vitro transcriptionTagsExpressionMutationDetailed in manuscriptPromoterT7 (inactivated)Available sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFRLuc-CoV2-delSS
Plasmid#177615PurposeDual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. Slippery sequence (SS) deleted (negative control).DepositorInsertSARS-CoV-2 FSE
UseLuciferaseTagsExpressionMammalianMutationSlippery sequence deletedPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV_hU6-sgRNA_hUbC-dCas9-ZIM3-KRAB-T2a-PuroR
Plasmid#172982Purposecontrol & cloning vector for CRISPRi expressing dead Cas9-ZIM3-KRAB fusionDepositorInsertcontrol sgRNA
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-FlipGFP(TEV cleavage seq) T2A mCherry
Plasmid#124429PurposeExpresses FlipGFP (TEV cleavage sequence) and T2A mCherry in mammalian cellsDepositorInsertFlipGFP(TEV cleavage seq) T2A mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
dCas9-VPR_P2A_mCherry
Plasmid#154193PurposeLentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry markerDepositorInsertsp-dCas9-VPR_P2A_mCherry
UseLentiviralTagsExpressionMammalianMutationD10A, H840APromoterEF1asAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro
Plasmid#71236PurposeExpress sgRNA and dCas9-KRAB from lentiviral vectorDepositorInsertshumanized dCas9-KRAB T2A Puro
sgRNA
UseCRISPR and LentiviralTagsFlagExpressionMutationD10A and H840APromoterAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only