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Plasmid#168026PurposeExpresses eCFP-VAMP5(M1-Q60)-mVenus in bacterial cellsDepositorInserteCFP-VAMP5(M1-Q60)-mVenus
UseTagsHisx6ExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-calpainsensor
Plasmid#36182Purposedetection of activity of ubiquitous calpainsDepositorInsertalpha-Fodrin cleavage site (Sptan1 Mouse)
UseTagseCFP and eYFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-InPAkt
Plasmid#181914PurposeIndicator of Phosphoinositides using Akt; genetically encoded FRET-based sensor for monitoring 3-phosphoinositide dynamics in living cells.DepositorInsertInPAkt
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-CVm-PTEN
Plasmid#168032PurposeExpresses eCFP-PTEN(M265-N329)-mVenus in bacterial cellsDepositorInserteCFP-PTEN(M265-N329)-mVenus
UseTagsHisx6 and mycExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-PlcR(K30/32L)
Plasmid#181831PurposePhospholipid binding-deficient Lyn-PlcR negative-control plasmid.DepositorInsertLyn-PlcR (K30L,K32L)
UseTagsECFP, EYFP, and N-terminal targeting sequence fro…ExpressionMammalianMutationLysines 30 and 32 in PH domain mutated to leucine.PromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL1 Aquamarine
Plasmid#106285PurposeExpresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoterDepositorInsertAquamarine
UseTags6xHis and TEV cleavage (N terminal on insert)ExpressionBacterialMutationT65S and H148GPromoterT7Available sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSM12
Plasmid#101643PurposeEncodes the fluorescent protein meffCFP for constitutive expression in E. coli.DepositorInsertmeffCFP
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-JNKAR1-NES
Plasmid#84632PurposeUnimolecular, FRET-based JNK activity reporter; cytosol targetedDepositorInsertECFP-FHA1-JNKsubDD-Citrine-NES
UseTags6xHis, Citrine, ECFP, Nuclear export signal (NES)…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPglu-600uDelta11 Aphrodite
Plasmid#13569DepositorInsertFLIPglu F16A Delta11 Aphrodite
UseTagsAphrodite and ECFPExpressionBacterialMutationFluorophore-derived linker shortened. F16A mutati…PromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRSM14
Plasmid#101645PurposeEncodes the fluorescent protein efasCFP for constitutive expression in E. coli.DepositorInsertefasCFP
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPglu-600uDelta13 Aphrodite
Plasmid#13570DepositorInsertFLIPglu F16A Delta13 Aphrodite
UseTagsAphrodite and ECFPExpressionBacterialMutationFluorophore-derived linker shortened. F16A mutati…PromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET-CVm-Sec22b
Plasmid#168029PurposeExpresses eCFP-Sec22b(A131-H190)-mVenus in bacterial cellsDepositorInserteCFP-Sec22b(A131-H190)-mVenus
UseTagsHisx6 and mycExpressionBacterialMutationPromoterAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-CVm-VAMP8
Plasmid#168028PurposeExpresses eCFP-VAMP8(G7-R67)-mVenus in bacterial cellsDepositorInserteCFP-VAMP8(G7-R67)-mVenus
UseTagsHisx6 and mycExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSM13
Plasmid#101644PurposeEncodes the fluorescent protein meleCFP for constitutive expression in E. coli.DepositorInsertmeleCFP
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tom20-CR
Plasmid#171461PurposeExpresses Tom20-CR, an anchor protein of iCMM/NiCMM, in mammalian cellsDepositorInsertTom20 (1-34 aa)-ECFP-FRB (2021-2113 aa)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAL204
Plasmid#22282PurposeExpresses PhyB-mCFP with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membraneDepositorInsertpCMV-PhyB(1-908)-10aaLinker-mCFP-Kras4BCT (PHYB Mustard Weed)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
Cytosolic Fyn FRET Biosensor
Plasmid#124654PurposeFRET biosensor. The highly sensitive biosensor has a mutated SH2 domain, ECFP and YPetDepositorInsertSynthetic gene containing ECFP-SH2 domain-substrate-linker-YPet
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
QUAS-jGCaMP7s-T2A-tdTomato
Plasmid#200010PurposeQUAS reporter line expressing GCaMP7s calcium indicator and tdTomato, separated by T2ADepositorInsert3xP3-ECFP-jGCaMP7s-td-Tomato
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAN357
Plasmid#120898PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt), multiple-cloning site for fusion to N-terminus of target proteinDepositorInsertcyan fluorescent protein, codon-optimized for low GC organisms, multiple-cloning site for protein fusion
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
NowGFP /pQE-30
Plasmid#74749PurposeNowGFP gene for bacterial expressionDepositorInsertNowGFP
UseTagsHisTag (with a mutation but still working)ExpressionBacterialMutationWasCFP with substitutions E6K, L42M, T43S, V68M, …PromoterT5Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only