Addgene

We narrowed to 3,973 results for: biorxiv

Showing: 961 - 980 of 3973 results

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pDONR221-CERS5

Plasmid

#166158

Purpose

Gateway entry vector for N. furzeri CERS5

Depositor

Daniel Jarosz

Article

Yiwen R. Chen et al BioRxiv

Insert

CERS5

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-DDX1

Plasmid

#166160

Purpose

Gateway entry vector for N. furzeri DDX1

Depositor

Daniel Jarosz

Article

Yiwen R. Chen et al BioRxiv

Insert

DDX1

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-DDX39A

Plasmid

#166161

Purpose

Gateway entry vector for N. furzeri DDX39A

Depositor

Daniel Jarosz

Article

Yiwen R. Chen et al BioRxiv

Insert

DDX39A

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-DDX3X

Plasmid

#166162

Purpose

Gateway entry vector for N. furzeri DDX3X

Depositor

Daniel Jarosz

Article

Yiwen R. Chen et al BioRxiv

Insert

DDX3X

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-DDX5

Plasmid

#166163

Purpose

Gateway entry vector for N. furzeriDDX5

Depositor

Daniel Jarosz

Article

Yiwen R. Chen et al BioRxiv

Insert

DDX5

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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PCR4-Hsp68::eGFP-H11

Plasmid

#211941

Purpose

dual-enSERT-1 eGFP vector for site-specific integration into the H11 locus (contains hsp68 minimal promoter)

Depositor

Evgeny Kvon

Article

Hollingsworth et al bioRxiv 2023.12.10.570890

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

HSP68

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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hTspan12(1-305)-1D4_pTT5

Plasmid

#216381

Purpose

Mammalian expression vector to transiently express full-length human Tspan12 with a Rho1D4 tag

Depositor

William Weis

Article

Bruguera et al bioRxiv 2024.02.03.578714

Insert

Tetraspanin-12 (TSPAN12 Human)

Use

Tags

Rho1D4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 msNLRP3(ΔPYD, I125M-END) (STOP)

Plasmid

#218640

Purpose

Gateway shuttling vector for cloning N-terminal NLRP3(ΔPYD, I125M-END) tags (contains a stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Gateway entry

Tags

Expression

Mutation

delPYD (I125M-END)

Promoter

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 hNLRP3 R262W (disease-associated mutation)

Plasmid

#218647

Purpose

Gateway shuttling vector for cloning NLRP3-R262W (no stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (NLRP3 Human)

Use

Gateway entry

Tags

Expression

Mutation

R262W

Promoter

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 msNLRP3(ΔPYD, I125M-END)

Plasmid

#218641

Purpose

Gateway shuttling vector for cloning N- and C-terminal NLRP3(ΔPYD, I125M-END) tags (contains no stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Gateway entry

Tags

Expression

Mutation

delPYD (I125M-END)

Promoter

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 msNLRP3 (STOP)

Plasmid

#218638

Purpose

Gateway shuttling vector for cloning N-terminal NLRP3 tags (contains a stop codon--note: bulky N-terminal tags are likely not functional)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Gateway entry

Tags

Expression

Mutation

Promoter

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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msHook2 g2 lentiCRISPRv2-mCherry

Plasmid

#218653

Purpose

Knockout vector for mouse Hook2

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Hook2 (Hook2 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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Ecoli PancACe

Plasmid

#215712

Purpose

Expresses acetyl-CoA biosensor in Ecoli

Depositor

Katharine Diehl

Article

Smith et al bioRxiv 2023.12.31.573774

Insert

PancACe

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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G555

Plasmid

#216483

Purpose

Expression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.

Depositor

Christophe Danelon

Insert

plsB, plsC, cdsA, pssA

Use

Cell-free expression

Tags

Expression

Mutation

Promoter

T7 for each gene

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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His-MBP-FL-Efg1

Plasmid

#216395

Purpose

Expresses MBP-tagged full length Efg1

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-FL

Use

Tags

6xHis tags-MBP

Expression

Bacterial

Mutation

Promoter

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD

Plasmid

#216396

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-ΔDBD

Use

Tags

6xHis-tags

Expression

Bacterial

Mutation

deleted amino acids 182-356

Promoter

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-N-PrLD

Plasmid

#216397

Purpose

Expresses 6xHis-tagged N-terminal prion-like domains of Efg1

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-N-PrLD

Use

Tags

6xHis-tags

Expression

Bacterial

Mutation

deleted amino acids 182-554

Promoter

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-C-PrLD

Plasmid

#216398

Purpose

Expresses 6xHis-tagged C-terminal prion-like domains of Efg1

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-C-PrLD

Use

Tags

6xHis-tags

Expression

Bacterial

Mutation

Promoter

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-DBD

Plasmid

#216399

Purpose

Expresses 6xHis-tagged DBD of Efg1

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-DBD

Use

Tags

6xHis-tags

Expression

Bacterial

Mutation

deleted amino acids 2-181 and 357-554

Promoter

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLV UBC APEX2-3xFLAG-IRES-eGFP

Plasmid

#214983

Purpose

Cytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments).

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

APEX2 (APX2 Glycine max (soybean))

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

UBC

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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