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Plasmid#107155PurposeMBP-rPKL construct for production and purification of rat PKL pS12DepositorInsertMBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
UseTagsC-term 6-his and N-term MBPExpressionBacterialMutationSerine 12 to TAG for phosphoserine insertionPromoterPBADAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
MBP-rPKL S113TAG
Plasmid#107157PurposeMBP-rPKL S113TAG for production and purification of rat PKL pS113DepositorInsertMBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
UseTagsC-term 6-his and N-term MBPExpressionBacterialMutationSerine 113 changed to TAG for phosphoserine inser…PromoterPBADAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
Fibronectin-human-mutated N-terminal (N) O-Glycosylation site
Plasmid#120404Purposeenables eukaryotic expression of human plasma fibronectin in which the first O-glycosylation site was mutated from threonine to serineDepositorInsertFibronectin (FN1 Human)
UseTagsExpressionMammalianMutationContaining complete variable region with a mutati…PromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Fibronectin-human-mutated V2 O-Glycosylation site
Plasmid#120405Purposeenables eukaryotic expression of human plasma fibronectin in which the second O-glycosylation site was mutated from threonine to serineDepositorInsertFibronectin (FN1 Human)
UseTagsExpressionMammalianMutationContains complete variable region with a mutation…PromoterCMVAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSIREN-RetroQ-BPTF-sh5
Plasmid#83045PurposeMMLV LTR plasmid pSIREN-RetroQ-BPTF-sh5DepositorUseRetroviralTagsExpressionMutationPromoterAvailable sinceOct. 24, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.3_SARS2_omicron BA.2
Plasmid#183700Purposeexpressing SARS-CoV-2 BA.2 spike protein for pseudovirus productionDepositorInsertSpike (BA.2) (S SARS-CoV-2)
UseLentiviral and RetroviralTagsExpressionMammalianMutationc-terminal 18 aa deletionPromoterCMVAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-NL63-D14
Plasmid#172666Purposeencodes NL63 spike protein, for pseudovirus productionDepositorInsertHCNV63gp2 (HCNV63gp2 human coronavirus NL63)
UseLentiviral and RetroviralTagsNAExpressionMammalianMutationc-terminal deletion of 14aaPromoterCMVAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pInducer-myc-ERK2-R67S/S153D
Plasmid#213597PurposeDoxycycline inducible expression of myc-tagged ERK2-R67S/S153D cDNA in mammilian cellsDepositorInsertMAPK1 (MAPK1 Human)
UseLentiviralTagsMYCExpressionMammalianMutationR67S, S153DPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_SARS2_omicron_BA.1
Plasmid#180375PurposePseudovirus plasmid expressing SARS-CoV-2 omicron BA.1 strain spike proteinDepositorInsertSpike (omicron BA.1 variant) (S SARS-CoV-2)
UseLentiviral and RetroviralTagsExpressionMammalianMutationc terminal D18PromoterCMVAvailable sinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-TAF15 Stop
Plasmid#84895PurposeDONOR vector for Gateway cloning of TAF15DepositorInsertTAF15 (TAF15 Human)
UseGateway donorTagsExpressionMutationPromoternoneAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJOP-HTT-HR65Q
Plasmid#92249PurposeHTT donor DNA with 65Q for homologous recombination-mediated gene modificationDepositorInsertHTT donor DNA (HTT Human)
UsePrecisionx hr targeting vectorTagsExpressionMutationBarcode of wobble bases to prevent TALEN cleaving…PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_SARS2_BA.4/5
Plasmid#186810Purposepseudovirus plasmid expressing BA.4 and BA.5 spike proteinDepositorInsert43740568 (S SARS-CoV-2)
UseTagsExpressionMammalianMutationHY69-70del, L452R, F486V, Q493R, on the basis of …PromoterCMVAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJOP-HTT-HR45Q
Plasmid#92248PurposeHTT donor DNA with 45Q for homologous recombination-mediated gene modificationDepositorInsertHTT donor DNA (HTT Human)
UsePrecisionx hr targeting vectorTagsExpressionMutationBarcode of wobble bases to prevent TALEN cleaving…PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-KrasG12D/sgKras/Cre
Plasmid#99851PurposeExpresses Cre-recombinase, a barcoded HDR template to introduce a G12D mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.DepositorInsertKras (Kras Mouse)
UseAAV and Cre/LoxTagsExpressionMammalianMutationAvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…PromoterAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-FLAG-SHLD2
Plasmid#114117PurposeExpresses N-terminally tagged FLAG-SHLD2 in mammalian cellsDepositorInsertSHLD2 (SHLD2 Human)
UseGenomic integration, tetracycline inducibleTagsFLAGExpressionMammalianMutationPromoterCMV/TetO2Available sinceAug. 28, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.3_SARS2_BA.2.12.1
Plasmid#186809Purposepseudovirus plasmid that expresses spike of BA.2.12.1DepositorInsertSpike (BA.2.12.1) (S SARS-CoV-2)
UseTagsExpressionMammalianMutationL452Q, S704L on the basis of BA.2, c-terminal 18 …PromoterCMVAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-KrasG12C/sgKras/Cre
Plasmid#99850PurposeExpresses Cre-recombinase, a barcoded HDR template to introduce a G12C mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.DepositorInsertKras (Kras Mouse)
UseAAV and Cre/LoxTagsExpressionMammalianMutationAvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…PromoterAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJOP-HTT-HR81Q
Plasmid#92250PurposeHTT donor DNA with 81Q for homologous recombination-mediated gene modificationDepositorInsertHTT donor DNA (HTT Human)
UsePrecisionx hr targeting vectorTagsExpressionMutationBarcode of wobble bases to prevent TALEN cleaving…PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJOP-HTT-HR30Q
Plasmid#92247PurposeHTT donor DNA with 30Q for homologous recombination-mediated gene modificationDepositorInsertHTT donor DNA (HTT Human)
UsePrecisionx hr targeting vectorTagsExpressionMutationBarcode of wobble bases to prevent TALEN cleaving…PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-KrasG12V/sgKras/Cre
Plasmid#99854PurposeExpresses Cre-recombinase, a barcoded HDR template to introduce a G12V mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.DepositorInsertKras (Kras Mouse)
UseAAV and Cre/LoxTagsExpressionMammalianMutationAvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…PromoterAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only