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Plasmid#168494PurposeMammalian expression of the K8,9A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertK8,9A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-rDAD2 cPGFP(N180)
Plasmid#166001PurposeExpression of coding sequence in Escherichia coliDepositorInsertrDAD2 cPGFP(N180)
UseTagsExpressionBacterialMutationLSSmOrange- V49A (please see depositor comments)PromoterT7 promoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-DAD2 cPGFP(A149)
Plasmid#165998PurposeExpression of coding sequence in Escherichia coliDepositorInsertDAD2 cPGFP(A149)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-12xLexAOp-sfGFP-MiniWhite
Plasmid#165916PurposeBlasticidin resistant 12xLexAOp sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable LexAOp Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_∆R13–DD-talin1 (1-2299)
Plasmid#166125PurposeFor expression of mouse talin-1 (residues 1-2299) with C-terminal deletion of dimerization domain and R13 (∆R13-DD) in mammalian cells. Contains C-terminal EGFP tag.DepositorInsert∆R13–DD-Talin-1 (Tln1 Mouse)
UseCmv promoterTagsEGFPExpressionMammalianMutationresidues 1-2299, deletion of dimerization domain …PromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
VP2C-GFP-MIOX only
Plasmid#166684PurposeYeast integrative plasmid for expressing mouse myo-inositol oxygenase fused to VP2C-GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertVP2C-GFP-MIOX
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL10Available sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
deltaVP1 + VP2C-GFP-MIOX
Plasmid#166682PurposeYeast integrative plasmid for expressing deltaVP1 (GAL1 promoter) and mouse myo-inositol oxygenase fused to VP2C-GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsVP2C-GFP-MIOX
Murine polyomavirus deltaVP1 (NLS deletion mutant)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Pet22b-mEGFP-YAP-C-IDR
Plasmid#166441PurposeBacterial expression of 6x His tagged mEGFP-YAP1-C-IDR fusion proteinDepositorInsertmEGFP, YAP-C-IDR(266-504) (YAP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-rShHTL7 cPGFP(E167)
Plasmid#166004PurposeExpression of coding sequence in Escherichia coliDepositorInsertrShHTL7 cPGFP(E167)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-rShHTL7 cPGFP(D165)
Plasmid#166003PurposeExpression of coding sequence in Escherichia coliDepositorInsertrShHTL7 cPGFP(D165)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-rShHTL7 cPGFP(C164)
Plasmid#166002PurposeExpression of coding sequence in Escherichia coliDepositorInsertrShHTL7 cPGFP(C164)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-DAD2 cPGFP(N180)
Plasmid#166000PurposeExpression of coding sequence in Escherichia coliDepositorInsertDAD2 cPGFP(N180)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-DAD2 cPGFP(A165)
Plasmid#165999PurposeExpression of coding sequence in Escherichia coliDepositorInsertDAD2 cPGFP(A165)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-HisDAD2 cPGFP(G164)
Plasmid#165997PurposeExpression of coding sequence in Escherichia coliDepositorInsertHisDAD2 cPGFP(G164)
UseTags6HisExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-rShHTL7 cPGFP(L166)
Plasmid#165995PurposeExpression of coding sequence in Escherichia coliDepositorInsertrShHTL7 cPGFP(L166)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-DAD2 cPGFP(G164)
Plasmid#165993PurposeExpression of coding sequence in Escherichia coliDepositorInsertDAD2 cPGFP(G164)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 SEC66-VAC8-GFP
Plasmid#166840PurposeExpresses ER localized Vac8-GFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.DepositorUseTagsGFP and SEC66 transmembrane domainExpressionYeastMutationSEC66 transmembrane domainPromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 VAC8-G2A-GFP
Plasmid#166837PurposeExpresses a myristoylation mutant Vac8-GFP in yeasts, mutation is Gly2AlaDepositorInsertVAC8 (VAC8 Budding Yeast)
UseTagsGFPExpressionYeastMutationG2APromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-31aa-Venus-pEGFP-C3
Plasmid#166754PurposeFor expression of mCerulean3 linked to Venus by 31 amino acid linkerDepositorInsertmCerulean3-31aa-Venus
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Venus-BimEL(Bik)-pEGFP-C1
Plasmid#166760PurposeTo examine effect of swapping the BH3 region in full length BimEL with that of BikDepositorUseTagsVenusExpressionMammalianMutationBH3 region removed and inserted BH3 of human Bik …PromoterCMVAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only