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Plasmid#185579Purposeto see protein dynamicsDepositorInsertBeta-catenin
UseIn vitro transcription (mrna synthesis)TagsGFPExpressionMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
ubc-nls-pcp-10xSunTag (SRv1.1)
Plasmid#185798Purposepcp-10xSunTag component for SunRISER SRv1.1DepositorInsertpcp-10xSunTag
UseTagsFactorXa site, HA, and NLSExpressionMammalianMutationPromoterhuman ubiquitin C promoterAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
ubc-nls-pcp-12xSunTag (SRv1.2)
Plasmid#185799Purposepcp-12xSunTag component for SunRISER SRv1.2DepositorInsertpcp-12xSunTag
UseTagsFactorXa site, HA, and NLSExpressionMammalianMutationPromoterhuman ubiquitin C promoterAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKP1193
Plasmid#167614PurposeExpress TonB H20NDepositorInserttonB H20N
UseTagsExpressionBacterialMutationtonB with H20N mutationPromoterAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
p7SKU6SBpur
Plasmid#160329Purposedual sh RNA vector expressing cfSBFP2 and a puromycin selection markerDepositorTypeEmpty backboneUseTagscfSBFP2ExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXf20pemIK-GW
Plasmid#126576PurposeVector for gene expression in Xylella fastidiosa, includes pemI/pemK toxin-antitoxin system for plasmid stability, Gateway cloning compatibleDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pISH-Zfhx4
Plasmid#84324PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Zfhx4. Not for gene expression.DepositorInsertZfhx4 (Zfhx4 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceNov. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pISH-Phtf1
Plasmid#84318PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Phtf1. Not for gene expression.DepositorInsertPhtf1 (Phtf1 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceNov. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pISH-Zhx1
Plasmid#74364PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Zhx1. Not for gene expression.DepositorInsertzinc fingers and homeoboxes 1 (Zhx1 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK290
Plasmid#71554PurposeProduction of Acetobacter aceti 1023 alanine racemase (alr) with double mutant (AaAlr-K39A,Y260A)DepositorInsertalanine racemase
UseTagsExpressionBacterialMutationchanges lysine-39 to alanine and tyrosine-260 to …PromoterT7Available sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK289
Plasmid#71553PurposeProduction of Acetobacter aceti 1023 alanine racemase (alr) with Lys39>Ala mutant (AaAlr-K39A)DepositorInsertalanine racemase
UseTagsExpressionBacterialMutationchanges lysine-39 to alanine (please see deposito…PromoterAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK326
Plasmid#71557PurposeProduction of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala368>Asp mutant (H6AaAlr-A368D)DepositorInsertalanine racemase
UseTagsHis6ExpressionBacterialMutationchanges alanine-368 to aspartatePromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK325
Plasmid#71556PurposeProduction of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala367>Asp mutant (H6AaAlr-A367D)DepositorInsertalanine racemase
UseTagsHis6ExpressionBacterialMutationchanges alanine-367 to aspartatePromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK345
Plasmid#71559PurposeProduction of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala367/Ala368 deletion (H6AaAlr-A367del,A368del)DepositorInsertalanine racemase
UseTagsHis6ExpressionBacterialMutationdeletes alanine-367 and alanine-368PromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK344
Plasmid#71558PurposeProduction of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala368 deletion (H6AaAlr-A368del)DepositorInsertalanine racemase
UseTagsHis6ExpressionBacterialMutationdeletes alanine-367 (same as deleting alanine-368)PromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (AAV Retrograde)
Viral Prep#154867-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (#154867). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterHuman Synapsin-1TagsmCherry (Flp-dependent)Available sinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-SomaGCaMP6f2
Plasmid#158756PurposeCell body-targeted GCaMP6f, under synapsin promoter: GCaMP6f followed by a linker, and the EE-RR coiled-coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.DepositorInsertSomaGCaMP6f2
UseAAVTagsExpressionMammalianMutationPromotersynapsin promoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EML4-ALK variant 3a I1171N
Plasmid#183832PurposeExpress EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N mutation in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of EML4 exon 1-6a and ALK exon…ExpressionMammalianMutationI1171NPromoterCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-SomaGCaMP7
Plasmid#158759PurposeCell body-targeted GCaMP7f, under synapsin promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.DepositorInsertSomaGCaMP7
UseAAVTagsExpressionMammalianMutationPromotersynapsin promoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only