Addgene

We narrowed to 21,112 results for: LEA

Showing: 9721 - 9740 of 21112 results

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pClneoMyc MOB1 T35A

Plasmid

#47937

Purpose

expresses Myc tagged human MOB1 containing T35A mutation

Depositor

Yutaka Hata

Article

Bao et al Genes Cells. 2009 Dec;14(12):1369-81. do

Insert

MOB1A (MOB1A Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

T35A

Promoter

CMV

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
MDH1-miR-324SM-PGK-GFP-Blast

Plasmid

#31518

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-324 (Mir324 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

seed mutant control

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
3704 pGEX4T-3 MLL AD L2854A

Plasmid

#8876

Depositor

Stanley Korsmeyer

Article

Ernst et al Mol Cell Biol. 2001 Apr . 21(7):2249-58.

Insert

MLL min A.D. (KMT2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

L2854A: ILPSDIMDFVL --> ILPSDIMDFVA

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pHIV-EF1a-Il1a-ZsGreen

Plasmid

#231987

Purpose

Express IL-1alpha-ZsGreen fusion protein

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

IL-1alpha-ZsGreen fusion construct

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-EYFP

Plasmid

#231927

Purpose

Cre-off/Flp-on EYFP under the Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

eYFP with introns

Use

AAV

Tags

Expression

Mutation

Promoter

Ef1a

Available since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-Puro-P2A-NES/PKI-mNeonGreen2x-NLS/FUS

Plasmid

#228400

Purpose

Encodes NES/PKI-mNeonGreen2x-NLS/FUS which is a tandem of mNeongreen with an N-terminal PKI NES and a C-terminal FUS NLS that shuttles between the nucleus and the cytoplasm

Depositor

Dirk Daelemans

Article

Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

Insert

NES/PKI-mNeonGreen2x-NLS/FUS

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4-CMV-NLS/cMyc-AcGFP2x-NES/iKB2-P2A-Puro

Plasmid

#228398

Purpose

Encodes NLS/cMyc-AcGFP2x-NES/iKB2 which is a tandem of AcGFP with an N-terminal cMyc NLS and a C-terminal iKB2 NES that shuttles between the nucleus and the cytoplasm

Depositor

Dirk Daelemans

Article

Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

Insert

NLS/cMyc-AcGFP2x-NES/iKB2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4-CMV-3xFLAG-NLS/SV40-AcGFP-NES/PKI-P2A-Puro

Plasmid

#228399

Purpose

Encodes 3xFLAG-NLS/SV40-AcGFP-NES/PKI which is a AcGFP with an N-terminal SV40 NLS and a C-terminal iKB2 NES that shuttles between the nucleus and the cytoplasm

Depositor

Dirk Daelemans

Article

Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

Insert

3xFLAG-NLS/SV40-AcGFP-NES/PKI

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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MLF2-GFP-3xFLAG

Plasmid

#230986

Purpose

Expresses GFP tagged MLF2 in mammalian cells

Depositor

Christian Schlieker

Article

Prophet et al Nat Cell Biol. 2022 Nov;24(11):1630-1641

Insert

MLF2 (MLF2 Human)

Use

Tags

3xFLAG and GFP

Expression

Mammalian

Mutation

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc:FRB:gs10:Cul1(dC501)

Plasmid

#228533

Purpose

A fragment of humanCul1 protein in fusion with FRB domain through a flexible glycine-serine linker.

Depositor

Roman Jerala

Article

Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

Insert

myc:FRB:gs10:Cul1(dC501) (CUL1 Synthetic, Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pWT-IFl_WT-IFr

Plasmid

#192207

Purpose

Wildtype I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Wildtype I-F Leader and wildtype I-F Locus DNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pScr-IFl_WT-IFr

Plasmid

#192208

Purpose

Scrambled I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Scrambled I-F Leader and wildtype I-F Locus DNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IFl_Con-IFr

Plasmid

#192209

Purpose

Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-F Leader and Consensus I-F Locus DNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IFl_Con-IEr

Plasmid

#192210

Purpose

Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-E repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-F Leader and Consensus I-E Locus DNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IFl_Con-ICr

Plasmid

#192211

Purpose

Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-C repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-F Leader and Consensus I-C Locus DNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IFl_Con-IIAr

Plasmid

#192212

Purpose

Consensus I-F CRISPR Leader and a CRISPR locus with two consensus II-A repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-F Leader and Consensus II-A Locus DNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IEl_Con-IEr

Plasmid

#192213

Purpose

Consensus I-E CRISPR Leader and a CRISPR locus with two consensus I-E repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-E Leader and Consensus I-E Locus DNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-ICl_Con-ICr

Plasmid

#192214

Purpose

Consensus I-C CRISPR Leader and a CRISPR locus with two consensus I-C repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-C Leader and Consensus I-C Locus DNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IIAl_Con-IIAr

Plasmid

#192215

Purpose

Consensus II-A CRISPR Leader and a CRISPR locus with two consensus II-A repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus II-A Leader and Consensus II-A Locus DNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pX1-IFl_Con-IFr

Plasmid

#192216

Purpose

Mutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

I-F leader and I-F repeat construct not studied further in this paper (X1)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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