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Plasmid#204213PurposeDoxycycline inducible lentiviral vector for human NKX2-1 (I207M) overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsExpressionMammalianMutationPoint mutation on I207MPromoterTetONAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1-Q175∗-EF1a-TagRFP-2A-tet3G
Plasmid#204211PurposeDoxycycline inducible lentiviral vector for human NKX2-1 (Q175∗) overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsExpressionMammalianMutationPoint mutation on Q175* (*termination)PromoterTetONAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1-R178∗-EF1a-TagRFP-2A-tet3G
Plasmid#204210PurposeDoxycycline inducible lentiviral vector for human NKX2-1 (R178∗) overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsExpressionMammalianMutationPoint mutation on R178* (*termination)PromoterTetONAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1-I207F -EF1a-TagRFP-2A-tet3G
Plasmid#204212PurposeDoxycycline inducible lentiviral vector for human NKX2-1 (I207F) overexpressionDepositorInsertNK2 homeobox 1 (NKX2-1 Human)
UseLentiviralTagsPoint mutation on I207FExpressionMammalianMutationPromoterTetONAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMT2-HA-RAPGEF2(S1244A/S1248A)
Plasmid#110163PurposeExpression of human RAPGEF2 (S1244A/S1248A) in mammalian cellsDepositorInsertRAPGEF2 (Rap guanine nucleotide exchange factor 2 (RAPGEF2 Human)
UseTagsHAExpressionMammalianMutationS1244A/S1248APromoterCMVAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
plIG-123_HA-GD2-28z_CAR_TF_Library
Pooled Library#207478PurposeHA-GD2-28z CAR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into thDepositorSpeciesHomo sapiensUseCRISPRAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
plIG-107_NY-ESO-1_TCR_TF_Library
Pooled Library#207475PurposeNY-ESO-1 TCR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into theDepositorSpeciesHomo sapiensUseCRISPRAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
plIG-110_CD19-BBz_CAR_TF_Library
Pooled Library#207477PurposeCD19-BBz CAR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into theDepositorSpeciesHomo sapiensUseCRISPRAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
plIG-132_CD19-28z_CAR_TF_and_SR_Library
Pooled Library#207481PurposeCD19-28z CAR in combination with 129 surface receptors, 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knocDepositorSpeciesHomo sapiensUseCRISPRAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIG-hNFATc2
Plasmid#74050Purposeretroviral expression plasmid for human NFATc2/CDepositorInserthuman NFATc2, isoform C (NFATC2 Human)
UseRetroviralTagsno tagExpressionMammalianMutationsilent mutation A1723C in the sequence of NM_1730…PromoterpMSCV-LTRsAvailable sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
FUW TetO HA-AMPKa2 WT
Plasmid#69826PurposeExpresses AMPK alpha 1 in mammalian cells in a doxycycline inducible mannerDepositorInsert5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterminimal CMV promoter with tetracycline operatorAvailable sinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-IRES-blasticidin-Sox2 S248A/T258A
Plasmid#182049Purposeexpression of Sox2 mutantsDepositorInsertSox2 (Sox2 Mouse)
UseTagsFLAGExpressionMammalianMutationS248A/T258APromoterCAGAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Flag‐mTim3 (T1 truncation)
Plasmid#49210Purposeexpresses Flag tagged mouse Tim3 (T1 truncation)DepositorInsertTim3 T1 truncation (Havcr2 Mouse)
UseTagsFLAG and signal sequenceExpressionMammalianMutationT1 truncation ( contains all but the three most C…PromoterEF1aAvailable sinceDec. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SEMA3D-COMP5AP-AviTag-9xHis
Plasmid#157530PurposeMammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.DepositorInsertSEMA3D (SEMA3D Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SaPE2-P2A-BSD
Plasmid#174818PurposeMammalian expression of SaCas9 prime editor 2 with P2A-BSD markerDepositorInsertSaPE2-P2A-BSD
UseTagsSV40 bpNLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag‐mTim3 (del.Cyto)
Plasmid#49209Purposeexpresses Flag tagged mouse Tim3 fragment (no cytosolic domain)DepositorInsertTim3 (del.Cyto) (Havcr2 Mouse)
UseTagsCD8 signal sequence and FLAGExpressionMammalianMutationcytosolic domain deleted, no Tim3 signal sequence…PromoterEf1aAvailable sinceDec. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
Flag‐mTim3 (T2 truncation)
Plasmid#49211Purposeexpresses Flag tagged mouse Tim3 (T2 truncation)DepositorInsertTim3 T2 truncation (Havcr2 Mouse)
UseTagsFLAG and signal sequenceExpressionMammalianMutationT2 truncation ( lacks all but one tyrosine, which…PromoterEF1aAvailable sinceDec. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSMPUW-miR-124-GFP-Puro
Plasmid#117321PurposeOverexpression of miR-124 in eurkaryotic cells, encoded in a human beta-globin intronDepositorInserthsa-miR-124-1 (MIR124-1 Human)
UseLentiviralTagsGFP-PuroExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-P2A-BSD
Plasmid#174819PurposeMammalian expression of SpCas9 prime editor 2 with P2A-BSD markerDepositorInsertPE2-P2A-BSD
UseTagsP2A-BSD and SV40 bpNLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-tetpA(hBCL2-miR9/9*-124)-iRPT
Plasmid#154881PurposeDoxycycline-dependently expresses human BCL2 and murine miR9/9*-124. Ubiquitously expresses iRFP713, Puromycin resistance gene, and rtTAM2.DepositorInsertsUseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only