Skip to main content
Addgene
Showing: 9621 - 9640 of 14766 results

  3. AAVS1-TRE-U2AF2-TurboID-HA-rtTA

    Plasmid
    #226997
    Purpose
    Integrative plasmid to express U2AF2-TurboID in mammalian cells
    Depositor
    Bruno Dallagiovanna
    Insert
    U2 small nuclear RNA auxiliary factor 2 (U2AF2 Human)
    Use
    Tags
    TurboID-HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    226997-plasmid-map-sequence-id-451922

    Enlarge

  9. pCMVMagFRET-1

    Plasmid
    #50742
    Purpose
    Expresses MagFRET-1 in the cytosol of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal cytosolic Ca2+ levels.
    Depositor
    Maarten Merkx
    Article
    Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:
    Insert
    gene encoding for MagFRET-1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50742-plasmid-map-sequence-id-327713

    Enlarge

  14. pGEMHE-NLS-mEGFP

    Plasmid
    #105527
    Purpose
    T7 promotor drives in vitro mRNA transcription of a mEGFP with a nuclear localisation signal
    Depositor
    Melina Schuh
    Article
    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31
    Insert
    Nucleoplasmin
    Use
    In vitro transcription
    Tags
    mEGFP
    Expression
    Mutation
    NLS of nucleoplasmin (encoding amino acids KRPAAT…
    Promoter
    T7
    Available since
    March 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    105527-plasmid-map-sequence-id-201844

    Enlarge

  15. GCEPIA1-SNAPER

    Plasmid
    #172659
    Purpose
    Detect the real-time ER calcium store and local calcium microdomains in live cells.
    Depositor
    Pingyong Xu
    Article
    Luo et al Biophys Rep (2019) 5, 31–42
    Insert
    calmodulin
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Please see depositor comments
    Promoter
    Available since
    Aug. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    172659-plasmid-map-sequence-id-339177

    Enlarge

  17. pREXC3GH

    Plasmid
    #47077
    Purpose
    FX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags
    Depositor
    Raimund Dutzler, Eric Geertsma
    Article
    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.
    Type
    Empty backbone
    Use
    Lactococcus lactis expression
    Tags
    3C protease site, GFP, and His
    Expression
    Mutation
    Promoter
    NIS (nisin)
    Available since
    Jan. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    47077-plasmid-map-sequence-id-67103

    Enlarge

  18. MCS+

    Plasmid
    #90412
    Purpose
    A FRET sensor that report the electric potential at the inner leaflet of cell plasma membrane
    Depositor
    Katharina Gaus
    Article
    Ma et al Nat Biotechnol. 2017 Mar 13. doi: 10.103
    Insert
    Lck10+ Venus+mcherry+ strech of positively charged amino acids
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    90412-plasmid-map-sequence-id-178958

    Enlarge

  20. pcDXC3GMS

    Plasmid
    #49031
    Purpose
    FX cloning mammalian cell line expression vector with CMV promoter and C-terminal 3C protease cleavage site, EGFP, Myc tag and streptavidin binding peptide
    Depositor
    Raimund Dutzler, Eric Geertsma
    Article
    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.
    Type
    Empty backbone
    Use
    Tags
    3C protease site (PreScission site), EGFP, MYC, a…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    49031-plasmid-map-sequence-id-71989

    Enlarge

Showing: 9621 - 9640 of 14766 results