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pLentiPGK Puro DEST p38KTRClover

Plasmid

#59152

Purpose

Lentiviral vector to express p38 KTR mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

p38 Kinase Translocation Reporter (MAPK14 Human, Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pET26b_AT118-L-V5-His

Plasmid

#222848

Purpose

E. coli periplasmid expression plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT118-L

Use

Tags

V5-His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti_EFS-Cas9-P2A-HygR

Plasmid

#164134

Purpose

Lentiviral construct for the expression of SpCas9 driven by EFS promoter in mammalian cells

Depositor

Hyongbum Kim

Article

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Insert

SpCas9 (cas9 Synthetic)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EFS

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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MTOR-F2108L_CD19-CAR-28z-2A-EGFP_AAV6_Donor

Plasmid

#211904

Purpose

Vector for AAV6 donor production. Targeted CD19-CAR-28z-2A-EGFP transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.

Depositor

Yannick Doyon

Article

Levesque et al bioRxiv 2023.09.14.557485

Insert

CD19-CAR-28z-2A-EGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hPGK1

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-5Nanog_2

Plasmid

#174871

Purpose

CRISPR vector for generating Nanog STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Nanog (Nanog Synthetic)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2

Plasmid

#135504

Purpose

Mammalian expression of myc-tagged HLA-A*02:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Use

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLV V5-hRnf185

Plasmid

#175127

Purpose

Lentiviral expression of V5-tagged human RNF185

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

RNF185 (RNF185 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCXLE-hMLN

Plasmid

#27079

Purpose

Non-integrating (episomal) expression of human C-MYC, LIN28 and NANOG

Depositor

Shinya Yamanaka

Article

Okita et al Nat Methods. 2011 May;8(5):409-12

Insert

C-MYC, LIN28, NANOG (MYC Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 6, 2011

Availability

Academic Institutions and Nonprofits only

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Fibronectin-human-plasma in pMAX

Plasmid

#120401

Purpose

enables eukaryotic expression of human plasma fibronectin

Depositor

Inaam Nakchbandi

Article

Rossnagl et al PLoS Biol. 2016 Sep 21;14(9):e1002562. d

Insert

Fibronectin (FN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pT3-N90-beta-catenin

Plasmid

#31785

Depositor

Xin Chen

Article

Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

Insert

beta-catenin (CTNNB1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Deletion of aa 1-90- Constitutitvely Active

Promoter

EF1alpha

Available since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

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mCherry-SH3(5R)

Plasmid

#112154

Purpose

Mammalian expression plasmid for SH3-5R in a vector expressing mCherry.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

SH3-5R (NCK1 Human)

Use

Tags

Expression

Mammalian

Mutation

only the second SH3 domain is expressed in this c…

Promoter

CMV

Available since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pT3-EF1aH N90-beta-catenin

Plasmid

#86499

Purpose

Mammalian expression of Myc tagged N90 beta-catenin

Depositor

Xin Chen

Article

Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

Insert

beta-catenin (CTNNB1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Deletion of amino acids 1-90; constitutively acti…

Promoter

EF1a

Available since

Feb. 16, 2017

Availability

Academic Institutions and Nonprofits only

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XLone-BSD Cas13d P2A mCherry

Plasmid

#155183

Purpose

PiggybacTransposon-based tunable and temporal expression control of Cas13d and mCherry

Depositor

Xiaoping Bao

Article

Chang et al Biomaterials. 2022 May 6;285:121569. doi

Insert

CasRx

Use

Unspecified; Piggybac transposon

Tags

NLS and P2A mCherry

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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IVT-donor_opt(R2Tg)-GFP

Plasmid

#223246

Purpose

In vitro transcription of the optimized R2Tg RNA donor

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg donor_opt

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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IVT-R2Tg_opt

Plasmid

#223247

Purpose

In vitro transcription of the optimized R2Tg protein

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg protein_opt

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)

Plasmid

#128604

Purpose

Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Inserts

24xMoonTag
kinesin family member 18b (KIF18B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Pcmv

Available since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pNICKclos1.0

Plasmid

#73639

Purpose

Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824

Depositor

Sheng Yang

Article

Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

Inserts

Cas9 nickase
sGRNA to pyrE

Use

E.coli-clostridium shuttle vector

Tags

Expression

Mutation

D10A

Promoter

j23119 and ptb

Available since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

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ER-CanlonicSF

Plasmid

#178343

Purpose

To explore endoplasmic reticulum lactate dynamics

Depositor

Alejandro San Martín

Article

Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

Insert

ER-CanlonicSF

Use

Tags

ER Retention Signal, ER Signal Exon 1, and ER Sig…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLIX403_UBAP2L_mCherry

Plasmid

#105286

Purpose

puro-selectable lentivirus vector for inducible expression of a UBAP2L-mCherry fusion protein

Depositor

Eugene Yeo

Article

Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

Insert

UBAP2L (UBAP2L Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet ON

Available since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pNICKclos2.0

Plasmid

#73228

Purpose

Genome editing for gene xylR (cbei-2385) in clostridium beijerinckii NCIMB 8052

Depositor

Sheng Yang

Article

Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

Inserts

Cas9 nickase
sgRNA to xylR

Use

E.coli - clostridium shuttle vector

Tags

Expression

Mutation

D10A

Promoter

Pj23119 and Pthl

Available since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

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