Addgene

We narrowed to 11,275 results for: pcDNA

Showing: 941 - 960 of 11275 results

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pcDNA3.1+-PAFAH1B1_R22-

Plasmid

#92431

Purpose

mammalian expression of PAFAH1B1 R22 mutant. The protein is truncated and non functional (negative control).

Depositor

Ralf Brandes, Matthias Leisegang

Article

Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

Insert

PAFAH1B1 (PAFAH1B1 Human)

Use

Tags

Expression

Mammalian

Mutation

R22 (stop codon in position 22)

Promoter

CMV

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 GW-mCit-PA

Plasmid

#113449

Purpose

ProteinA-mCitrine Gateway shuttle vector for C-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Gateway shuttle vector

Tags

mCitrine-ProteinA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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tubulin-ReNL_pcDNA3

Plasmid

#89530

Purpose

Red color variant of bright luminescent protein, fused to b-tubulin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

ReNL

Use

Tags

b-tubulin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sapphireCKAR(T/A)

Plasmid

#118469

Purpose

Negative-control mutant for sapphireCKAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

sapphireCKAR(T/A)

Use

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNAIIIB rhoA QL

Plasmid

#61632

Purpose

mammalian expression of rhoA constitutively active mutant

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

rhoA (RHOA Human)

Use

Tags

Expression

Mammalian

Mutation

Q63L, constitutively active

Promoter

CMV

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-GSTM5

Plasmid

#11986

Depositor

Steven Johnson

Insert

GSTM5 (GSTM5 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
mSECFP/pcDNA3

Plasmid

#51951

Purpose

mammalian expression of mSECFP, a monomeric super enhanced cyan fluorescent protein

Depositor

Takeharu Nagai

Article

Matsuda et al Nat Methods. 2008 Apr;5(4):339-45. doi:

Insert

mSECFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ING4

Plasmid

#13289

Depositor

Curtis Harris

Article

Shiseki et al Cancer Res. 2003 May 15. 63(10):2373-8.

Insert

ING4 (ING4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-hNox3

Plasmid

#58341

Purpose

expresses human Nox3 in mammalian cells

Depositor

Botond Banfi

Article

Kiss et al Curr Biol. 2006 Jan 24;16(2):208-13.

Insert

NADPH oxidase 3 (NOX3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Ndel1

Plasmid

#12571

Depositor

Li-Huei Tsai

Article

Shu et al Neuron. 2004 Oct 14. 44(2):263-77.

Insert

Ndel1 (NDEL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-pnGFP

Plasmid

#64913

Purpose

Encodes a Fluorescent Reporter for Peroxynitrite in Mammalian Cells

Depositor

Huiwang Ai

Article

Chen et al J Am Chem Soc. 2013 Oct 9;135(40):14940-

Insert

pnGFP

Use

Tags

6xHis

Expression

Mammalian

Mutation

Chromophore tyrosine of the protein mutated to TA…

Promoter

CMV

Available since

June 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3.3TOPO-T7-hCas9

Plasmid

#161876

Purpose

In vitro transcription of SpCas9

Depositor

Stephane Pelletier

Article

Pelletier et al Immunity. 2015 Jan 20;42(1):18-27. doi:

Insert

human codon optimized SpCas9

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcdna3 T7 JIP3a

Plasmid

#53457

Purpose

interacts with MAPK signaling module

Depositor

Roger Davis

Article

Kelkar et al Mol Cell Biol. 2000 Feb;20(3):1030-43.

Insert

mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)

Use

Tags

T7

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(-)-LZ-CD95L

Plasmid

#104349

Purpose

Expresses human CD95L leucine zipper in mammalian cells

Depositor

Alexander McLellan

Article

Rad S M et al PLoS One. 2020 Jul 24;15(7):e0232915. do

Insert

CD95L leucine zipper (FASLG Human)

Use

Tags

octahistidine tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf5(WT)-HA

Plasmid

#79427

Purpose

Expresses C-terminally HA-tagged Arf5(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Hanai et al J Biochem. 2016 Feb;159(2):201-8. doi: 1

Insert

Arf5 (ARF5 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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Lyn-OeNL_pcDNA3

Plasmid

#89528

Purpose

Orange color variant of bright luminescent protein, targeted to the plasma membrane

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Use

Tags

Lyn

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-BREP

Plasmid

#172337

Purpose

BRET-Based Bioluminescent Red Protein

Depositor

Huiwang Ai

Article

Tian et al Nat Commun. 2022 Jul 8;13(1):3967. doi:

Insert

BREP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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NF-155 pcDNA3

Plasmid

#197290

Purpose

Plasmid expressing an antigen targeting the Plasmid expressing an antigen targeting Neurofascin-155

Depositor

Peter Brophy

Article

Collinson et al Glia. 1998 May;23(1):11-23

Insert

Pan-Neurofascin (extracellular) (Nfasc Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoLAMA-G97

Plasmid

#130705

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-G97

Use

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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Twitch-2C pcDNA3

Plasmid

#104501

Purpose

fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-2C

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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