Addgene

We narrowed to 20,514 results for: crispr

Showing: 941 - 960 of 20514 results

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pCas4_1_2_VA

Plasmid

#186438

Purpose

Encodes Cas4, Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4, Cas1 and Cas2

Use

CRISPR

Tags

Expression

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pCas4_1_Δ2_VA

Plasmid

#186439

Purpose

Encodes Cas4, Cas1, leader, repeat and one spacer from F. Novicida (type V-A)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4, Cas1 and Cas2

Use

CRISPR

Tags

Expression

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pCas-mut4_1_2_VA

Plasmid

#186441

Purpose

Encodes Cas4 (K70A), Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4, Cas1 and Cas2

Use

CRISPR

Tags

Expression

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pCas-4/1_2_VB

Plasmid

#186442

Purpose

Encodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4/1 and Cas2

Use

CRISPR

Tags

Expression

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pCas-4/1Δ2_VB

Plasmid

#186443

Purpose

Encodes Cas4/1, leader, repeat and one spacer from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4/1

Use

CRISPR

Tags

Expression

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pCas-mut4/1_2_VB

Plasmid

#186444

Purpose

Encodes Cas4(K81A)/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4/1 and Cas2

Use

CRISPR

Tags

Expression

Mutation

Cas4 domain (K81A)

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pdCas12a(RuvC)

Plasmid

#186447

Purpose

Encodes catalytically inactive Cas12a (D917A, E1006A) from F. novicida (type V-A)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

dCas12a

Use

CRISPR

Tags

Expression

Mutation

D917A, E1006A

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pCas12(PI)

Plasmid

#186448

Purpose

Encodes inactive Cas12a containing mutation in the PI domain (K613A, K617A) from F. novicida (type V-A)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas12a (PI)

Use

CRISPR

Tags

Expression

Mutation

K613A, K617A

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pdCas12b(RuvC)

Plasmid

#186450

Purpose

Encodes catalytically inactive Cas12b (E848A, D977A) from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

dCas12b

Use

CRISPR

Tags

Expression

Mutation

E848A, D977A

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pCas12b(PI)

Plasmid

#186451

Purpose

Encodes inactive Cas12b containing mutation in the PI domain (R122A, G143P) from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas12b (PI)

Use

CRISPR

Tags

Expression

Mutation

R122A, G143P

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pML104-HygMx4

Plasmid

#83475

Purpose

Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.

Depositor

Patricia Wittkopp

Insert

HphMX4

Use

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pML104-KanMx4

Plasmid

#83476

Purpose

Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.

Depositor

Patricia Wittkopp

Insert

KanMX4

Use

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pML104-NatMx3

Plasmid

#83477

Purpose

Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains NatMX3 marker for yeast transformation.

Depositor

Patricia Wittkopp

Insert

NatMx3

Use

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
MLM3636

Plasmid

#43860

Purpose

guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoter

Depositor

Keith Joung

Insert

None

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pCAMTA2-donor

Plasmid

#132501

Purpose

CRISPR donor plasmid to create GFP fusion proteins

Depositor

Kevin White

Insert

CAMTA2 (CAMTA2 Human)

Use

CRISPR

Tags

GFP

Expression

Mutation

none

Promoter

Availability

Academic Institutions and Nonprofits only

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pPITX2-donor

Plasmid

#176372

Purpose

CRISPR donor plasmid to create GFP fusion proteins

Depositor

Kevin White, Barbara Stranger

Insert

PITX2 (PITX2 Human)

Use

CRISPR

Tags

GFP

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pcrRNA.con

Plasmid

#61285

Purpose

Constitutive repeat-only E. coli CRISPR array

Depositor

Chase Beisel

Article

Luo et al Nucleic Acids Res. 2014 Oct 17. pii: gku

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Availability

Academic Institutions and Nonprofits only

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pCMV-ACME

Plasmid

#186717

Purpose

ACME dual-deaminase CRISPR base editor

Depositor

Xue Gao

Article

Yuan et al Nat Commun. 2022 May 19;13(1):2771. doi:

Insert

ACME

Use

CRISPR

Tags

Nucleoplasmin NLS

Expression

Mammalian

Mutation

See manuscript

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCFD3-ebony

Plasmid

#83380

Purpose

Drosophila gRNA expression plasmid targets ebony

Depositor

Richard Padgett

Article

Kane et al G3 (Bethesda). 2017 Jan 5;7(1):87-93. do

Insert

ebony (e Fly)

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pWJ66

Plasmid

#46570

Purpose

Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)

Depositor

Luciano Marraffini

Article

Bikard et al Nucleic Acids Res. 2013 Jun 12.

Inserts

tracrRNA
dcas9-w
CRISPR array

Use

CRISPR

Tags

rpoZ

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Availability

Academic Institutions and Nonprofits only

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