-
Plasmid#98404PurposeEntry vector for S. pombe CRISPR-Cas9 system. The gRNA can be integrated easily through Gibson Assembly with the plasmid backbone digested by Not1DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only
-
pMZ374
Plasmid#59896PurposeCombination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).DepositorInsertsCas9
rrk1:sgRNA
UseCRISPRTagsExpressionYeastMutationSilent muation of the CspCI sitePromoteradh1 and rrk1Available sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAH237
Plasmid#121146PurposeExpression of both nmt41p-cas9 and rrk1p-gRNA (LEU2 marker) for CRISPR genome editing in fission yeastDepositorInsertsgRNA cassette
humanized Streptococcus pyogenes Cas9
UseCRISPRTagsFlagExpressionYeastMutationPromoterS.pombe rrk1 and nmt41Available sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDCC5
Plasmid#59984PurposeBi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassetteDepositorInsertCas9-D10A
UseCRISPRTagsHAExpressionInsectMutationD10APromoterhsp70BbAvailable sinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pST_116_LVL2 cam
Plasmid#179332PurposeNT-CRISPR plasmid for a single gRNA.DepositorInsertPtac tfoX, Ptet cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_119_LVL2 cam
Plasmid#179333PurposeNT-CRISPR plasmid for integration of multiple gRNAs.DepositorInsertPtac tfoX, Ptet cas9, PJ23106 acrIIA4, sfGFP dropout to be replaced with gRNAs
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCIP-mB2M
Plasmid#154091PurposeSelf-Cutting and Integrating CRISPR backbone targeting murine B2MDepositorInsertsgRNA and BAIT targeting murine B2M locus
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSCIP-hTRAC
Plasmid#154094PurposeSelf-Cutting and Integrating CRISPR backbone targeting human TCR-alpha common chainDepositorInsertsgRNA and BAIT targeting human TRAC locus
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFD3.1-w-dU6:3gRNA
Plasmid#123366PurposegRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).DepositorInsertU6:3-gRNA
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTFAM.1.0-gDNA
Plasmid#132443PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertTFAM (TFAM Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pRARB.1.0-gDNA
Plasmid#132471PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertRARB (RARB Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZNF3_1
Plasmid#72363PurposeEncodes gRNA for 3' target of human ZNF3 along with Cas9 with 2A GFPDepositorInsertZNF3 (ZNF3 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZNF3_2
Plasmid#72364PurposeEncodes gRNA for 3' target of human ZNF3 along with Cas9 with 2A GFPDepositorInsertZNF3 (ZNF3 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas4_1_2_VA
Plasmid#186438PurposeEncodes Cas4, Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)DepositorInsertCas4, Cas1 and Cas2
UseCRISPRTagsExpressionMutationPromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pdCas12a(RuvC)
Plasmid#186447PurposeEncodes catalytically inactive Cas12a (D917A, E1006A) from F. novicida (type V-A)DepositorInsertdCas12a
UseCRISPRTagsExpressionMutationD917A, E1006APromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas4_1_Δ2_VA
Plasmid#186439PurposeEncodes Cas4, Cas1, leader, repeat and one spacer from F. Novicida (type V-A)DepositorInsertCas4, Cas1 and Cas2
UseCRISPRTagsExpressionMutationPromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas-mut4_1_2_VA
Plasmid#186441PurposeEncodes Cas4 (K70A), Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)DepositorInsertCas4, Cas1 and Cas2
UseCRISPRTagsExpressionMutationPromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas-4/1_2_VB
Plasmid#186442PurposeEncodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)DepositorInsertCas4/1 and Cas2
UseCRISPRTagsExpressionMutationPromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas-4/1Δ2_VB
Plasmid#186443PurposeEncodes Cas4/1, leader, repeat and one spacer from A. acidoterrestris (type V-B)DepositorInsertCas4/1
UseCRISPRTagsExpressionMutationPromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only