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pET28B-PU.1-ETS-Loop

Plasmid

#124551

Purpose

Chimeric ETS domain: Loop of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 216 to 223 replaced with corresponding s…

Promoter

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28B-PU.1-ETS-Loop

Plasmid

#124551

Purpose

Chimeric ETS domain: Loop of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 216 to 223 replaced with corresponding s…

Promoter

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-PU.1-ETS N236H

Plasmid

#124616

Purpose

Chimeric ETS domain: N236H

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (ETS1, SPI1 Human)

Use

Tags

Expression

Mammalian

Mutation

Residues 236 altered from asparagine to a histidi…

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-PU.1-ETS N236H

Plasmid

#124616

Purpose

Chimeric ETS domain: N236H

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (ETS1, SPI1 Human)

Use

Tags

Expression

Mammalian

Mutation

Residues 236 altered from asparagine to a histidi…

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-PU.1-ETS N236Q

Plasmid

#124615

Purpose

Chimeric ETS domain: N236Q

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (SPI1, ETS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Residues 236 altered from asparagine to a glutami…

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-PU.1-ETS N236Q

Plasmid

#124615

Purpose

Chimeric ETS domain: N236Q

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (SPI1, ETS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Residues 236 altered from asparagine to a glutami…

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-PU.1-ETS N236Y

Plasmid

#124614

Purpose

Chimeric ETS domain: N236Y

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (SPI1, ETS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Residues 236 altered from asparagine to a Tyrosine

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-PU.1-ETS N236Y

Plasmid

#124614

Purpose

Chimeric ETS domain: N236Y

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (SPI1, ETS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Residues 236 altered from asparagine to a Tyrosine

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28B-PU.1-ETS-S3

Plasmid

#124550

Purpose

Chimeric ETS domain: S3 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 242 to 246 replaced with corresponding s…

Promoter

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28B-PU.1-ETS-S3

Plasmid

#124550

Purpose

Chimeric ETS domain: S3 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 242 to 246 replaced with corresponding s…

Promoter

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-PU.1-ETS-H2

Plasmid

#123358

Purpose

Chimeric ETS domain: H2 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 207 to 215 replaced with corresponding s…

Promoter

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-PU.1-ETS-H2

Plasmid

#123358

Purpose

Chimeric ETS domain: H2 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 207 to 215 replaced with corresponding s…

Promoter

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PGK2 gRNA (BRDN0001145595)

Plasmid

#76946

Purpose

3rd generation lentiviral gRNA plasmid targeting human PGK2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PGK2 (PGK2, PGK1P2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PGK2 gRNA (BRDN0001149179)

Plasmid

#76947

Purpose

3rd generation lentiviral gRNA plasmid targeting human PGK2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PGK2 (PGK2, PGK1P2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PGK2 gRNA (BRDN0001146354)

Plasmid

#76948

Purpose

3rd generation lentiviral gRNA plasmid targeting human PGK2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PGK2 (PGK2, PGK1P2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PGK2 gRNA (BRDN0001145595)

Plasmid

#76946

Purpose

3rd generation lentiviral gRNA plasmid targeting human PGK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PGK2 (PGK2, PGK1P2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PGK2 gRNA (BRDN0001149179)

Plasmid

#76947

Purpose

3rd generation lentiviral gRNA plasmid targeting human PGK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PGK2 (PGK2, PGK1P2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PGK2 gRNA (BRDN0001146354)

Plasmid

#76948

Purpose

3rd generation lentiviral gRNA plasmid targeting human PGK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PGK2 (PGK2, PGK1P2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST40-ARL4D-V5-His6

Plasmid

#67447

Purpose

Mammalian expression of hArl4D with C-term V5 and His6 tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4D (ARL4D Human)

Use

Tags

His and V5

Expression

Mammalian

Mutation

bp 272 A to C; N91T mutation; bp 327 T to G; sil…

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST40-ARL4D-V5-His6

Plasmid

#67447

Purpose

Mammalian expression of hArl4D with C-term V5 and His6 tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4D (ARL4D Human)

Use

Tags

His and V5

Expression

Mammalian

Mutation

bp 272 A to C; N91T mutation; bp 327 T to G; sil…

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only