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Plasmid#124643PurposeMyofilament localised red genetically encoded calcium sensor in mammalian expression vector used for AdEasy adenoviral DNA recombination.DepositorInsertRGECO-TnT (TNNT2 Human, Synthetic)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-FLAG
Plasmid#60360PurposePlasmid for cloning and expression of FLAG-GFP tagged proteins (C-terminal tag). Confers resistance to G418.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsEGFP and FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAT9679-sgRNA-BFP
Plasmid#162988PurposesgRNA cloning plasmidDepositorInsertexchangeable cassette
UseCRISPRTagsExpressionBacterial and MammalianMutationPromoterU6Available sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
811 pSG5L HA PTEN C124S
Plasmid#10744DepositorInsertPTEN (PTEN Human)
UseTagsHAExpressionMammalianMutationC124SPromoterAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
Flag-hPIASx beta
Plasmid#15210DepositorInsertPIASx beta (PIAS2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pATetO 6XHis T4 DNA Ligase
Plasmid#206207PurposeInducible expression of T4 DNA ligaseDepositorInsertT4 DNA ligase
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBBR1k-J23101-GFPuv
Plasmid#106388PurposepBBR1k-GFPuv with Anderson promoter J23101 expressing lacIDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBJG1 T7-8xHis-OGT K842M
Plasmid#154272PurposeBacterial expression of full length human OGT with K842M mutation (catalytically inactive). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.DepositorInsertO-GlcNAc Transferase (OGT Human)
UseTags8x His, HRV3C protease site, and T7 leaderExpressionBacterialMutationLysine 842 changed to Methionine- bacterial codon…PromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (AAV Retrograde)
Viral Prep#162379-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (#162379). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8f-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of the ultrafast calcium sensor, GCaMP8f. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSCMV:tdTomato
Plasmid#30530Purposemammalian expression of tdTomato controlled by the ubiquitously active CMV promoter.DepositorHas ServiceCloning Grade DNAInserttdTomato
UseTagsExpressionMammalianMutationR415HPromoterAvailable sinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMTL3
Plasmid#127966Purposeconstitutive expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locusDepositorInsertAAVS1-CAG-KRAB-dCas9-BFP-KRAB
UseTALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2_IRES2_CyOFP1_KCNQ2 var4
Plasmid#173155PurposeFor mammalian expression of KCNQ2 and creating variantsDepositorInsertKCNQ2 (KCNQ2 Human)
UseTagsIRES2-CyOFP1ExpressionMammalianMutationPromoterSimian CMV IE94Available sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mDlx-ChR2-mCherry-Fishell-3 (AAV Retrograde)
Viral Prep#83898-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-mDlx-ChR2-mCherry-Fishell-3 (#83898). In addition to the viral particles, you will also receive purified pAAV-mDlx-ChR2-mCherry-Fishell-3 plasmid DNA. mDlx-driven expression of ChR2 fused to mCherry. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromotermDlxTagsmCherryAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
p6350 MSCV-CMV-CMV-Flag-HA-ATAD5
Plasmid#31356DepositorInsertATAD5 (ATAD5 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
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Integrin β4-GFP
Plasmid#205092PurposeExpress GFP-tagged human integrin β4 in mammalian cellsDepositorInsertIntegrin beta 4 (ITGB4 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3431
Plasmid#144907PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNR3C2 (NR3C2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMTL5
Plasmid#127967PurposeDHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locusDepositorInsertAAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB
UseTALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJFRC201-10XUAS-FRT>STOP>FRT-myr::smGFP-HA
Plasmid#63166PurposeGAL4-responsive UAS "flp-On" spaghetti monster GFP-HA reporterDepositorInsert"spaghetti monster GFP-HA"
UseTagsN-myristoylationExpressionInsectMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
TFORF0431
Plasmid#142622PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertAIRE (AIRE Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only