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Plasmid#129383PurposePlasmid bearing the editing target of APOBEC1 fused between the mCherry and the Tag-BFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of Tag-BFPDepositorInsertmCherry-ApoB-Tag-BFP
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSETa-Amrose v3
Plasmid#79539PurposepRSETa (Invitrogen) plasmid expressing Amrose variant 3DepositorInsertAmrose v3
UseTags6xHis, T7 epitope, and Xpress tagExpressionBacterialMutationPromoterT7Available sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
SGL40C.EFS.RFP657
Plasmid#69147PurposeAdvanced lentiviral Vector for sgRNA (hU6 Promoter) delivery with RFP657 expression (EFS Promoter)DepositorInsertssgRNA
EFS
tagRFP657
UseTagsExpressionMutationPromoterAvailable sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-GTF2F2
Plasmid#31323DepositorInsertgeneral transcription factor IIF (GTF2F2 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pIhh742bp-Luc
Plasmid#53605PurposeReporter construct containing a 742 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assaysDepositorInsertIhh promoter
UseLuciferase; ReporterTagsExpressionMutationPromoterAvailable sinceJune 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAimR
Plasmid#98687PurposeExpresses AimR gene from the B. subtilis phage phi3T, with its native promoter, C' His-tagDepositorInsertAimR
UseTagsHis-tagExpressionBacterialMutationPromoterNativeAvailable sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_hPGK1_mScarlet-I-TOSI_Donor
Plasmid#178087PurposeHDR donor plasmid to introduce the mScarlet-I mTOR signaling indicator (TOSI) cassette to AAVS1 using AAVS1 T2 sgRNA.DepositorInsertmScarlet-I mTOR signaling indicator (TOSI)
UseCRISPRTagsExpressionMammalianMutationPromoterHuman PGK1Available sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
p6613 MSCV-IP N-HAonly RBCK1
Plasmid#35011DepositorInsertRBCK1 (RBCK1 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterMSCV LTRAvailable sinceMarch 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
hSTARR-seq_SCP1 vector_blocking 3
Plasmid#99318PurposeVector to measure enhancer activity of candidates from a DNA library by STARR-seq.DepositorTypeEmpty backboneUseHstarr-seq screening vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf221
Plasmid#12909PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf221
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 29, 2006AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-1+t
Plasmid#48147PurposeShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1EDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter EtsA site mutant pGL3Basic
Plasmid#32729DepositorInsertCCND1 promoter (CCND1 Human)
UseLuciferase; Luciferase reporter geneTagsExpressionMutation-822 EtsA site AGGAA deletionPromoter-962 CCND1 promoterAvailable sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
PcDNA-DEST47-CypB-Del3 C-term GFP
Plasmid#36136DepositorInsertcyclophilin B (PPIB Human)
UseTagsGFPExpressionMammalianMutationcontains amino acids 39-216 of cyclophilin B; G63…PromoterCMVAvailable sinceNov. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
YFP-Gamma 5
Plasmid#36044DepositorInsertG protein subunit Gamma 5 (Gng5 Rat)
UseTagsHis and YFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-KCNIP2
Plasmid#34799DepositorInsertKv channel interacting protein 2 (KCNIP2 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET15-His-∆NGlpG
Plasmid#155131PurposeExpresses N-terminally His-tagged E. coli GlpG (residues 87-276) from pET15bDepositorInsertGlpG (glpG Escherichia coli)
UseTagsHisExpressionBacterialMutationinsert codes for GlpG residues 87-276PromoterT7Available sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046931
Plasmid#145706PurposeBacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;FLAG_2ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
Tmem98-mTq2
Plasmid#124504PurposeExpresses mTurquoise2-tagged mouse TMEM98 in mammalian cells (C-terminal tag)DepositorInsertTmem98 (Tmem98 Mouse)
UseTagsmTurquoise2ExpressionMammalianMutationstop codon was removed to allow fusion with sGFP2…PromoterCMVAvailable sinceMay 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-CAG-F14F15S-BFP2-(mCherry)'
Plasmid#115235PurposeLentiviral transfer plasmid for expression of mTagBFP2 before Flp recombination and mCherry after itDepositorInsertmTagBFP2 and mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter EtsB site mutant pGL3Basic
Plasmid#32730DepositorInsertCCND1 (CCND1 Human)
UseLuciferaseTagsExpressionMutation-778 EtsB binding site AGGAA deletionPromoter-962 CCND1Available sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only