-
Plasmid#15555DepositorInsertDPY30 (DPY30 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EngPD
Plasmid#64039PurposeMammalian expression of paired domain of Pax8 fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)DepositorInsertPax8 paired domain (Pax8 Mouse)
UseTagsEng (N-terminal repressor domain of the drosophiā¦ExpressionMammalianMutationpaired domain, aa 1-147PromoterCMVAvailable sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
Px458-Cas9n-Trp73-Exon4-3sgRNA
Plasmid#88850PurposeCRISPR KO of Trp73DepositorInsertTrp73 (Trp73 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
Px458-Cas9n-Trp73-Exon4-4sgRNA
Plasmid#88851PurposeCRISPR KO of Trp73DepositorInsertTrp73 (Trp73 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)mGAT1-0-CFP
Plasmid#41664DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)
UseTagsCFPExpressionMammalianMutationāMonomerizingā CFP A206K mutation; C-terminal hydā¦PromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(-) + SLNCR1 _462-572 (conserved sequence)
Plasmid#86818Purposemammalian expression of lncRNA LINC00673/SLNCR1 deletion constructDepositorInsertLINC00673 (LINC00673 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 T538N
Plasmid#60537PurposeDonor vector for human Pak5 T538N mutantDepositorInsertPak5 (PAK5 Human)
UseGateway donor vectorTagsExpressionMutationT538NPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 S573N
Plasmid#60538PurposeDonor vector for human Pak5 S573N mutantDepositorInsertPak5 (PAK5 Human)
UseGateway donor vectorTagsExpressionMutationS573NPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAV115.loxP
Plasmid#63187PurposepRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV116.loxP
Plasmid#63188PurposepRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG415-ADH1p-HA-rap1-K9R
Plasmid#84531PurposeThis plasmid can be used to express HA-tagged Rap1-K9R from the ADH promoter in yeastDepositorInsertRAP1 (RAP1 Budding Yeast)
UseTagsHAExpressionYeastMutationK(43,61,69,117,240,246,527,582,651)RPromoterADH1Available sinceJan. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG415-GPDp-EGFP-rap1-K3NR
Plasmid#84533PurposeThis plasmid can be used to express EGFP-tagged Rap1K3NR from the GDP promoter in yeastDepositorInsertRAP1 (RAP1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationK(43,117,118)RPromoterGPDAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG415-GPDp-EGFP-rap1-K3CR
Plasmid#84534PurposeThis plasmid can be used to express EGFP-tagged Rap1K3CR from the GDP promoter in yeastDepositorInsertRAP1 (RAP1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationK(462,463,651)RPromoterGPDAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG415-GPDp-EGFP-rap1-K9R
Plasmid#84535PurposeThis plasmid can be used to express EGFP-tagged Rap1K9R from the GDP promoter in yeastDepositorInsertRAP1 (RAP1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationK(43,61,69,117,240,246,527,582,651)RPromoterGPDAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-rap1-K462-463-651R
Plasmid#84564PurposeThis plasmid can be used as a donor for subcloning into Gateway destination vectors.DepositorInsertRAP1 (RAP1 Budding Yeast)
UseGateway donor plasmidTagsExpressionMutationK462-463-651RPromoternoneAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-rap1-K43-117-118R
Plasmid#84565PurposeThis plasmid can be used as a donor for subcloning into Gateway destination vectors.DepositorInsertRAP1 (RAP1 Budding Yeast)
UseGateway donor plasmidTagsExpressionMutationK43-117-118RPromoternoneAvailable sinceOct. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS CRABP1
Plasmid#19189DepositorInsertCellular retinoic acid binding protein 1 (CRABP1 Chicken)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
XE125 XDsh delta COOH-GFP-CS2+
Plasmid#16784DepositorInsertXdsh-delta-COOH (dvl2.L Frog)
UseXenopus expressionTagsGFPExpressionMutationcontains Dsh amino acids 1-588 (deleting 599-736)PromoterAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCVL TAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers
Plasmid#50421PurposepTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.DepositorTypeEmpty backboneUseLentiviral and TALENTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
p5M-PR-B-1-688
Plasmid#89115Purposemammalian expression of human progesterone receptor amino acid residues 1-688 in pCMV5 with 5 restriction sites deleted in polylinkerDepositorInserthuman progesterone receptor-B N-term+DNA binding domain (PGR Human)
UseTagsExpressionMammalianMutation5 restriction sites deleted in polylinker, contaiā¦PromoterCMVAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only