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Plasmid#184775PurposeYeast expression of Aur1 under GAL promoter. Uses antibiotic resistance marker hphMX6.DepositorInsertproGAL-Aur1 (AUR1 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
UG76-pLYS2-GFP-PKCbeta
Plasmid#184762PurposeMarker for DAG. Yeast expression of human PKC beta under LSY2 promoter. Uses auxotrophic marker TRP1(Kluyveromyces lactis).DepositorInsertpLYS2-GFP-PKCbeta (PRKCB Human, Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GLRX-roGFP2 in pT3TS-Dest
Plasmid#194297PurposeIn vitro transcription of the redox sensor GLRX-roGFP2 from the T3 promoter. Parton lab clone KRKDepositorInsertGLRX-roGFP2
UseIn vitro transcription of mrnaTagsExpressionMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rtn2b-RHD272-472-GFP
Plasmid#186606PurposeEncodes human Reticulon 2 isoform B reticulon homology domain aa 272 to 472 fused to GFPDepositorInsertHuman Reticulon 2 isoform B amino acids 272 to 472 (RTN2 Human)
UseTagsmNeonGreenExpressionMammalianMutationMet-Gly-Ser-Lys peptide added at N-terminus for c…PromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT GFP CL1 DAmP
Plasmid#182513PurposeC-SWAT compatible vector for C-terminal tagging with GFP CL1 followed by the DAmP cassette. The stop codon after the CL1 degron is immediately followed by the Hygromycin B (Hph) resistance cassette.DepositorInsertCL1 and DAmP
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsXRN1_1630-1706_O
Plasmid#147096PurposeMammalian Expression of HsXRN1_1630-1706DepositorInsertHsXRN1_1630-1706 (XRN1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP1a_1-132_O
Plasmid#147089PurposeMammalian Expression of HsDCP1a_1-132DepositorInsertHsDCP1a_1-132 (DCP1A Human)
UseTagsExpressionMammalianMutationone silent mutation T237C compared to the sequenc…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP1a_132-582_O
Plasmid#147090PurposeMammalian Expression of HsDCP1a_132-582DepositorInsertHsDCP1a_132-582 (DCP1A Human)
UseTagsExpressionMammalianMutationone silent mutation T1716G compared to the sequen…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsXRN1_1459-1596_O
Plasmid#147093PurposeMammalian Expression of HsXRN1_1459-1596DepositorInsertHsXRN1_1459-1596 (XRN1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsXRN1_1597-1670_O
Plasmid#147094PurposeMammalian Expression of HsXRN1_1597-1670DepositorInsertHsXRN1_1597-1670 (XRN1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsXRN1_1597-1706_O
Plasmid#147095PurposeMammalian Expression of HsXRN1_1597-1706DepositorInsertHsXRN1_1597-1706 (XRN1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsRCK-mut1_N
Plasmid#147023PurposeMammalian Expression of HsRck-mut1DepositorInsertHsRck-mut1 (DDX6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-Nterm6Wmut_O
Plasmid#147068PurposeInsect Expression of DmGW182-Nterm6WmutDepositorInsertDmGW182-Nterm6Wmut (gw Fly)
UseTagsExpressionInsectMutationTwo non silent mutations V704A and N799S and one …PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-delM1_N
Plasmid#146974PurposeInsect Expression of DmGW182-delM1DepositorInsertDmGW182-delM1 (gw Fly)
UseTagsExpressionInsectMutationTwo non silent mutations V704A and N799S and one …PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot6_1-188_K
Plasmid#146738PurposeMammalian Expression of HsNot6_1-188DepositorInsertHsNot6_1-188 (CNOT6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot6_165-557_K
Plasmid#146739PurposeMammalian Expression of HsNot6_165-557DepositorInsertHsNot6_165-557 (CNOT6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP1a_131-582_L
Plasmid#146819PurposeMammalian Expression of HsDCP1a_131-582DepositorInsertHsDCP1a_131-582 (DCP1A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-SBP-C1_J
Plasmid#146655PurposeMammalian Expression vectorDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-delDUF_H
Plasmid#146437PurposeInsect Expression of DmGW182-delDUFDepositorInsertDmGW182-delDUF (gw Fly)
UseTagsExpressionInsectMutationTwo non silent mutations V704A and N799S and one …PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsRCK-K135A_H
Plasmid#146455PurposeMammalian Expression of HsRck-K135ADepositorInsertHsRck-K135A (DDX6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only