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pRSF_his6_sumo_SARS_CoV2_NSP14_P335H

Plasmid

#213525

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation P335H

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Use

Tags

His6-sumo

Expression

Bacterial

Mutation

changed P335 to H

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_H424R

Plasmid

#213526

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation H424R

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Use

Tags

His6-sumo

Expression

Bacterial

Mutation

changed H424 to R

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pUC18_BCR_GUSB_ABL1_e14a3

Plasmid

#190885

Purpose

molecular monitoring of atypical BCR::ABL1 fusion transcripts

Depositor

Nicholas Cross

Article

Schafer et al J Cancer Res Clin Oncol. 2021 Oct;147(10

Inserts

e14a3 BCR::ABL1 fusion transcript
ABL1 cDNA (ABL1 Human)
GUSB cDNA (GUSB Human)
BCR (BCR Human)

Use

Cloned cdna transcripts for use in rt-qpcr bcr::a…

Tags

Expression

Mutation

Promoter

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4-eIF3B MBE3 mutant

Plasmid

#133310

Purpose

Expression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 3

Depositor

Josep Domingo-Domenech

Article

Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

Insert

eIF3B promoter MBE3 mutant (EIF3B Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

ccacgtgacc changed to cAaAAAAacc

Promoter

eIF3B

Available since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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B-95.ΔAΔfabR

Bacterial Strain

#197934

Purpose

BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

Depositor

Kensaku Sakamoto

Article

Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

Bacterial Resistance

None

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only
B-95.ΔA

Bacterial Strain

#197933

Purpose

BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

Depositor

Kensaku Sakamoto

Article

Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

Bacterial Resistance

None

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only
Strain: C321.ΔA

Bacterial Strain

#68306

Purpose

Genomically recoded strain of E. coli (no genomic TAG)

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Bacterial Resistance

Bleocin (zeocin)

Available since

Sept. 29, 2015

Availability

Academic Institutions and Nonprofits only
39R861+

Bacterial Strain

#119737

Purpose

Six stable plasmids can be used as size standards

Depositor

Ruth Hall

Article

Moran et al Plasmid. 2019 Mar;102:6-9. doi: 10.1016/

Bacterial Resistance

None

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only
DH10B-ALT

Bacterial Strain

#61151

Purpose

DH10B modified to constitutively express araC, lacI, and tetR, integrated at the attB site on the E Coli genome. This is DH10B-ALT-Tet with the tetracycline resistance cassette deleted.

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Bacterial Resistance

None

Available since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only
C321.ΔClpS.Ub-UAG-sfGFP

Bacterial Strain

#98565

Purpose

Recoded strain C321.ΔA (MutS inactivated) with genomically integrated Ubiquitin-UAG-sfGFP reporter and ClpS inactivated

Depositor

George Church

Article

Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11

Bacterial Resistance

Ampicillin

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only
peGFP-N1-Cofilin

Plasmid

#186747

Purpose

Expresses wildtype GFP-tagged human cofilin in mammalian cells

Depositor

Geert van den Bogaart

Article

Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

Insert

Cofilin-1 (CFL1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS/XBB.1.5-RBD

Plasmid

#213038

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/XBB.1.5-RBD (S )

Use

Tags

6 His Tag

Expression

Mammalian

Mutation

G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

Promoter

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-EGFP-ALDH1A1-Neo

Plasmid

#189742

Purpose

The construct was used to express GFP tagged human ALDH1A1 in mammalian cells for the analysis of the subcellular location of this protein.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

ALDH1A1 (ALDH1A1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Omicron.BA.5-RBD

Plasmid

#213478

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.BA.5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/Omicron.BA.5 RBD (S )

Use

Tags

6 His Tag

Expression

Mammalian

Mutation

G339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…

Promoter

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS/EG5-RBD

Plasmid

#213410

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.EG5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/EG5-RBD (S )

Use

Tags

6 His Tag

Expression

Mammalian

Mutation

G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

Promoter

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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peGFP-N1-Cofilin-S41E

Plasmid

#186748

Purpose

Expresses S41E phosphomimetic GFP-tagged human cofilin in mammalian cells

Depositor

Geert van den Bogaart

Article

Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

Insert

Cofilin-1 (CFL1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Changed serine 41 to glutamic acid

Promoter

CMV

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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peGFP-N1-Cofilin-S41A

Plasmid

#186749

Purpose

Expresses S41A phosphodead GFP-tagged human cofilin in mammalian cells

Depositor

Geert van den Bogaart

Article

Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

Insert

Cofilin-1 (CFL1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Changed serine 41 to alanine

Promoter

CMV

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pmCherry-N1-cofilin

Plasmid

#186750

Purpose

Expresses wildtype mCherry-tagged human cofilin in mammalian cells

Depositor

Geert van den Bogaart

Article

Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

Insert

Cofilin-1 (CFL1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pmCherry-N1-Cofilin-S41E

Plasmid

#186751

Purpose

Expresses S41E phosphomimetic mCherry-tagged human cofilin in mammalian cells

Depositor

Geert van den Bogaart

Article

Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

Insert

Cofilin-1 (CFL1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Changed serine 41 to glutamic acid

Promoter

CMV

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR223-PARK7H126A

Plasmid

#115180

Purpose

Gateway cloning of PARK7H126A into any destination vector

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PARK7H126A (PARK7 Human)

Use

Gateway cloning into appropriate destination vect…

Tags

Expression

Mutation

p.H126A

Promoter

Available since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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