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Plasmid#117326PurposeDual luciferase assay negative control for miR-124 bindingDepositorInsertreverse miR-124 sponge
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast SHMT2 CD
Plasmid#106302PurposeExpresses SHMT2 K280A catalytic site mutantDepositorInsertserine hydroxymethyltransferase 2 (SHMT2 Human)
UseRetroviralTagsExpressionMammalianMutationK280A catalytic site mutation, Silent mutations d…PromoterAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTER GAMMA-TUBULIN shRNA Human
Plasmid#87955Purposereduced the expression of gamma-tubulin in human cellsDepositorInsertsh gamma-tubulin (TUBG1 Human)
UseTagsNo-tagExpressionMammalianMutationThe protein is a sh-gamma-tubulin resistant gene.…PromoterAvailable sinceOct. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
Flrt2-AP-His
Plasmid#71948PurposeExpresses the extracellular region of the FLRT2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertFlrt2 (Flrt2 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-hMeCP2-R270X
Plasmid#48092PurposeBacterial expression of Mxe intein/chitin binding domain tagged MeCP2-R270XDepositorInsertMethyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
UseTagsExpressionBacterialMutationMxe intein/chitin binding domain tagged, expresse…PromoterAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-hMeCP2-G273X
Plasmid#48093PurposeBacterial expression of Mxe intein/chitin binding domain tagged MeCP2-G273XDepositorInsertMethyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
UseTagsExpressionBacterialMutationMxe intein/chitin binding domain tagged, expresse…PromoterAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSCV-NSD3-short del(280-342)
Plasmid#72550Purposeexpresses 3*FLAG tagged human NSD3-short with 280-342 aa deletionDepositorInsertNSD3-short (NSD3 Human)
UseRetroviralTags3*FLAGExpressionMammalianMutation280-342 aa deletionPromoterLTRAvailable sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-NSD3-short del(384-645)
Plasmid#72549Purposeexpresses 3*FLAG tagged human NSD3-short with 384-645 aa deletionDepositorInsertNSD3-short (NSD3 Human)
UseRetroviralTags3*FLAGExpressionMammalianMutation384-645 aa deletionPromoterLTRAvailable sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-RUNX2(+10A)-IDR-mCherry
Plasmid#145287PurposeBacterial Expression of 6xHis RUNX2(+10A) IDR mCherry Fusion ProteinDepositorInsertRUNX2(+10A)-IDR (RUNX2 Human)
UseTagsExpressionBacterialMutationA-repeat expandedPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR-sgC1orf27-1
Plasmid#86130PurposeLentiviral vector expressing Cas9 and an sgRNA targeting C1orf27DepositorInsertsgRNA 1 targeting C1orf27 (C1orf27 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMX-Gal4-hMeCP2-R270X-R111G
Plasmid#48086PurposeMammalian expression of Gal4 tagged MeCP2-R270X-R111GDepositorInsertMethyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
UseTagsGal4ExpressionMammalianMutationR111G mutant MeCP2, expresses AT-hook R270XPromoterAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
PPBP-bio-His
Plasmid#52074PurposeExpresses full-length Platelet basic protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPPBP (PPBP Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
FCER2-His-bio
Plasmid#52336PurposeExpresses full-length Low affinity immunoglobulin epsilon Fc receptor ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.DepositorInsertFCER2 (FCER2 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSCV-NSD3-short del(100-263)
Plasmid#72548Purposeexpresses 3*FLAG tagged human NSD3-short with 100-263 aa deletionDepositorInsertNSD3-short (NSD3 Human)
UseRetroviralTags3xFlag and SV40 NLSExpressionMammalianMutation100-263 aa deletionPromoterLTRAvailable sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVTHM-mBrn2-3xflag
Plasmid#206390PurposeExpresses 3xflag fused mouse BRN2 in mammalian cells, for lentivirus generation.DepositorInsertBrn2 (Pou3f2 Mouse)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmMe31B-Q294AN296F-dsRNAres_W
Plasmid#147862PurposeInsect Expression of DmMe31B-Q294AAN296F-dsRNAresDepositorInsertDmMe31B-Q294AAN296F-dsRNAres (me31B Fly)
UseTagsExpressionInsectMutationone silent mutation A1101G compared to the sequen…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmMe31B-Q294AN296FR347A-dsRNAres_W
Plasmid#147863PurposeInsect Expression of DmMe31B-Q294AAN296FR347A-dsRNAresDepositorInsertDmMe31B-Q294AAN296FR347A-dsRNAres (me31B Fly)
UseTagsExpressionInsectMutationone silent mutation A1101G compared to the sequen…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmMe31B-R347A-dsRNAres_W
Plasmid#147864PurposeInsect Expression of DmMe31B-R347A-dsRNAresDepositorInsertDmMe31B-R347A-dsRNAres (me31B Fly)
UseTagsExpressionInsectMutationone silent mutation A1101G compared to the sequen…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmMe31B-S291A-dsRNAres_W
Plasmid#147865PurposeInsect Expression of DmMe31B-S291A-dsRNAresDepositorInsertDmMe31B-S291A-dsRNAres (me31B Fly)
UseTagsExpressionInsectMutationone silent mutation A1101G compared to the sequen…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5x3-hMeCP2-delN256-G273X
Plasmid#48090PurposeBacterial expression of GST tagged AT-Hook2-G273XDepositorInsertMethyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
UseTagsGSTExpressionBacterialMutationdeleted the N-terminal 256 codons, expresses AT-H…PromoterAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only