PPBP-bio-His
(Plasmid
#52074)
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PurposeExpresses full-length Platelet basic protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 52074 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTT3
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Backbone manufacturerYves Durocher, NRC
- Backbone size w/o insert (bp) 6618
- Total vector size (bp) 7022
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Modifications to backboneRat CD4 d3+4, enzymatic biotinylation sequence, linker and His tag (CD4d3+4-BioLHis).
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePPBP
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Alt namePlatelet basic protein precursor
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SpeciesH. sapiens (human)
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Insert Size (bp)404
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Entrez GenePPBP (a.k.a. B-TG1, Beta-TG, CTAP-III, CTAP3, CTAPIII, CXCL7, LA-PF4, LDGF, MDGF, NAP-2, PBP, SCYB7, TC1, TC2, TGB, TGB1, THBGB, THBGB1)
- Promoter CMV
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Tags
/ Fusion Proteins
- rat CD4 d3+4 (C terminal on backbone)
- enzymatic biotinylation sequence (C terminal on backbone)
- His tag (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer CMV-fwd (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byInsert was chemically synthesised.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Article ref 137
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PPBP-bio-His was a gift from Gavin Wright (Addgene plasmid # 52074 ; http://n2t.net/addgene:52074 ; RRID:Addgene_52074) -
For your References section:
A human platelet receptor protein microarray identifies FcepsilonR1alpha as an activating PEAR1 ligand. Sun Y, Vandenbriele C, Kauskot A, Verhamme P, Hoylaerts MF, Wright GJ. Mol Cell Proteomics. 2015 Feb 23. pii: mcp.M114.046946. 10.1074/mcp.M114.046946 PubMed 25713122