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Plasmid#172566PurposeInducible gene expression, pACYC origin, LacI repressor, PLlacO-1 promoter, mRFP, Chloramphenicol Resistance Marker.DepositorInsertLacI-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLlacO-1Available sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pELlR6
Plasmid#172562PurposeInducible gene expression, pET origin, LacI repressor, PLlacO-1 promoter, mRFP, Streptomycin Resistance Marker.DepositorInsertLacI-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLlacO-1Available sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSNAPC5-donor
Plasmid#176341PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertSNAPC5 (SNAPC5 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDLlR6
Plasmid#172559PurposeInducible gene expression, pCDF origin, LacI repressor, PLlacO-1 promoter, mRFP, Streptomycin Resistance Marker.DepositorInsertLacI-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLlacO-1Available sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCLlR2
Plasmid#172553PurposeInducible gene expression, pCOLA origin, LacI repressor, PLlacO-1 promoter, mRFP, Kanamycin Resistance Marker.DepositorInsertLacI-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLlacO-1Available sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pELlR4
Plasmid#172545PurposeInducible gene expression, pET origin, LacI repressor, PLlacO-1 promoter, mRFP, Carbenicillin Resistance Marker.DepositorInsertLacI-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLlacO-1Available sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
HCP9
Plasmid#166111PurposeThis plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Hta2DepositorInsertHta2-sg18 (HTA2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pROS15_MATz
Plasmid#166100PurposeExpression of dual gRNA targeting the MAT locusDepositorInsertMAT Z1
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Talin1-FERM
Plasmid#110563Purposebacterial expression of Talin 1DepositorInsertTalin 1 (Tln1 Mouse)
UseTags(His)8ExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
cfSYFP2-N
Plasmid#110631PurposeCys-free yellow fluorescent protein for extracellular imaging. SYFP2 variant (C48S/C70M). Molecular brightness is almost comparable to SYFP2.DepositorInsertcfSYFP2-N
UseTagsMulti-cloning sitesExpressionMammalianMutationCys48Ser and Cys70/MetPromoterAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZNF449-donor
Plasmid#132515PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertZNF449 (ZNF449 Human)
UseCRISPRTagsGFPExpressionMutationnonePromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pENTR-L4R1-PHtetO2
Plasmid#139526PurposeProvides conditional promoter cassette and PGK promoter driven puromycin resistant gene cassette between attL4-attR1 on Gateway entry vectorDepositorInserttetO2 promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
T18m
Plasmid#121037PurposeMoClo golden gate assembly DE part for L3S2P21 (382x-strength synthetic terminator with BsaI cut site removed; see DOI: 10.1038/nmeth.2515).DepositorInsertmodified L3S2P21 (synthetic Voigt terminator)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
V35m
Plasmid#121042PurposeMoClo golden gate assembly AE destination vector DVCK-AE-GFP (ColE1/kan resistance destination vector with constitutive GFP expression for screening.DepositorInsertKan destination vector with ColE1 origin and GFP selection
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
U17m
Plasmid#121023PurposeMoClo golden gate assembly BC part for Bujard RBS (strong RBS from https://doi.org/10.1093/nar/25.6.1203). Please see Supplemental Documents for annotated Genbank file.DepositorInsertBujard RBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
U19m
Plasmid#121024PurposeMoClo golden gate assembly BC part for MG-aptamer (fluorescent RNA aptamer of malachite green; see DOI: 10.1021/ja0529788) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertMG-apt
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
T15m
Plasmid#121034PurposeMoClo golden gate assembly DE part for L3S3P11 (173x-strength synthetic terminator, modified to remove BsaI cut site; see DOI: 10.1038/nmeth.2515).DepositorInsertmodified L3S3P11 (synthetic Voigt terminator)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C85m
Plasmid#120996PurposeMoClo golden gate assembly CD part for sigmaX (Bacillus subtilis RNA polymerase involved in heat response and biofilm formation). Please see Supplemental Documents for annotated Genbank file.DepositorInsertSigma X (ECF20_992)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C91m
Plasmid#120999PurposeMoClo golden gate assembly CD part for sfCFP (superfolder CFP developed by Ye Chen in Matthew Bennet's lab) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertsfCFP (Chen, Bennett)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only