Addgene

We narrowed to 51,996 results for: cloning

Showing: 921 - 940 of 51996 results

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C453-E17: attB4-PGKp>-attB5

Plasmid

#162976

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using the human PGK promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human pGK promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E09: attB4-Grp78p>-attB5

Plasmid

#162974

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using the human Grp78 promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human Glucose response protein 78 promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E36: attB4-CMV51p>-attB1r

Plasmid

#162927

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using an optimized human CMV promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

optimized CMV promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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C232-E25: attB2r-mVenus-attB3

Plasmid

#162905

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal mVenus fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Venus fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C232-E34: attB2r-iRFP670-attB3

Plasmid

#162908

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal iRFP670 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

far-red iRFP670 fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E11: attB5r-mTFP1-nostop-attB1r

Plasmid

#162942

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mTFP1 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Teal fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E19: attB4-mPol2p>-attB1r

Plasmid

#162971

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

murine RNA polymerase 2 promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E11: attB4-Grp78p>-attB1r

Plasmid

#162970

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human Glucose response protein 78 promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E10: attB4-FerHp>-attB1r

Plasmid

#162969

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human Ferritin heavy chain promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E34: attB5r-iRFP670-nostop-attB1r

Plasmid

#162947

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal iRFP670 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

far-red iRFP670 fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E29: attB5r-mKate2-nostop-attB1r

Plasmid

#162946

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mKate2 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Kate2 fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E15: attB5r-mVenus-nostop-attB1r

Plasmid

#162943

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mVenus fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Venus fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C415-E01: attB4-stuffer-attB1r

Plasmid

#162931

Purpose

Gateway attB4-attB1r entry clone containing a non-transcribed/translated region for use in generating negative control vectors

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

stuffer fragment for vector controls

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E26: attB4-SV40p>-attB1r

Plasmid

#162923

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the SV40 promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

SV40 immediate early promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E21: attB4-PGKp>-attB1r

Plasmid

#162921

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the human PGK promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human pGK promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C234-E07: attB2r-HA-attB3

Plasmid

#162911

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal HA epitope tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

HA epitope tag

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C234-E06: attB2r-FLAG-attB3

Plasmid

#162910

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal FLAG epitope tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

FLAG epitope tag

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C234-E05: attB2r-V5-attB3

Plasmid

#162909

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal V5 epitope tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

V5 epitope tag

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pFD-MBP-CS(TEV)-StrepTag-II-AI(TVMV)

Plasmid

#137110

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

MBP-CS(TEV)-StrepTag-II-AI(TVMV)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMOD_C2616

Plasmid

#91090

Purpose

Module C, Promoter: At7SL, Gene: BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)

Use

CRISPR

Tags

Expression

Mutation

Promoter

At7SL

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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