Showing: 921 - 940 of 14048 results
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Plasmid#173766PurposeFluorescent labeling of the nuclear envelopeDepositorInsertPDGF receptor TM domain, Biotin protein ligase
UseTagsFLAG and Signal peptideExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pBCCP-GFP-NLS
Plasmid#173923PurposeFluorescent labeling of the nuclear envelopeDepositorInsertBiotin carboxyl carrier protein, AcGFP1
UseTags3xNLSExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pET23PI-Sce ESARC
Plasmid#195607PurposeExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoterDepositorInsertPI-SceI
UseTagsExpressionBacterialMutationC552T,C554A, A557T,G584T, C588A, G878T, C881T, C9…PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET23PI-Sce ESARC D218A
Plasmid#195608PurposeExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D218A active site mutationDepositorInsertPI-SceI
UseTagsExpressionBacterialMutationT928C, T929C, A942CPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pJRTCLCrVA.008
Plasmid#22841DepositorInsertA DNA (with MCS)
UseCotton vigs vectorTagsExpressionBacterialMutationCoat Protein (AR1 gene) of Cotton Leaf Crumple Vi…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pJRTCLCrVB1.3
Plasmid#22844DepositorInsertB DNA
UseCotton vigs vectorTagsExpressionBacterialMutation1.3 unit B componet length inserted into pBlueScr…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pCVL TAL(Nd154C63) SFFV Xba-Sal linker
Plasmid#50420PurposepTAL plasmid compatible with the Golden Gate TALEN cloning system. For cloning of megaTALs or other TAL-fusions and expression in mammalian cells. Meganuclease/fusion is cloned between Xba-Sal sites.DepositorTypeEmpty backboneUseLentiviral and TALENTagsExpressionMammalianMutationPromoterSFFVAvailabilityAcademic Institutions and Nonprofits only -
pBJG1 T7-8xHis-OGT D554N
Plasmid#154279PurposeBacterial expression of full length human OGT with D554N mutation (Cleavage, no Ser/Thr glycosylation). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.DepositorInsertO-GlcNAc Transferase
UseTags8x His, HRV3C protease site, and T7 leaderExpressionBacterialMutationAspartate 554 changed to Asparagine- bacterial co…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pRN3P-NLS-EGFP-Btgh-3NLS
Plasmid#194469PurposeEncodes for bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.DepositorInsertBtGH84
UseFor in vitro transcription from t3 promoter after…TagsSV40 Nuclear Localization Signal, SV40 Nuclear Lo…ExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAcrVIB1
Plasmid#184566PurposeInducible expression of anti-CRISPR protein AcrVIB1 in bacteria.DepositorInsertanti-CRISPR protein inhibiting Cas13b nucleases
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pAcrVIB1-cloDF-kan
Plasmid#184836PurposeExpression of anti-CRISPR protein AcrVIB1 in bacteria.DepositorInsertanti-CRISPR protein inhibiting Cas13b nucleases
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119AvailabilityAcademic Institutions and Nonprofits only -
pAcrVIB1-cloDF-amp
Plasmid#184843PurposeExpression of anti-CRISPR protein AcrVIB1 in bacteria.DepositorInsertanti-CRISPR protein inhibiting Cas13b nucleases
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23119AvailabilityAcademic Institutions and Nonprofits only -
SUMO_MT3
Plasmid#200303PurposeBacterial expression plasmids for production of recombinant metallothionein 3 containing N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubilityDepositorInsertmetallothionein 3 (MT3 Human)
UseTagsSmt3ExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
SUMO_LPETG_MT3
Plasmid#200304PurposeBacterial expression plasmids for sortase mediated purification of recombinant MT3. Contains His-tag for purification and SUMO-tag for better solubility and LPETG for sortase mediated cleavageDepositorInsertmetallothionein 3 (MT3 Human)
UseTagsSmt3ExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
eSrtA-MT4
Plasmid#200306PurposeBacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT4. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtADepositorInserteSrtA-LPETG-MT4 (MT4 Human)
UseTagsHis-tag and SUMO-tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pJRTCLCrVA009
Plasmid#22842DepositorInsertA DNA containing CHLI fragment
UseCotton vigs vectorTagsExpressionBacterialMutationCotton Leaf Crumple Virus Coat Protein (AR1 gene)…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pJRTCLCrVA010
Plasmid#22843DepositorInsertA DNA containing GFP
UseCotton expression vectorTagsExpressionBacterialMutationCotton Leaf Crumple Virus A component with Coat P…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pCVL TAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers
Plasmid#50421PurposepTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.DepositorTypeEmpty backboneUseLentiviral and TALENTagsExpressionMammalianMutationPromoterSFFVAvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-EGFP-NLS
Plasmid#86677PurposeExpresses nuclear-localized GFP after lentiviral transduction. Can be used to monitor GFP leakage into the cytosol following nuclear envelope rupture events.DepositorInsertEGFP-NLS
UseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pRN3P-NLS-EGFP-deadBtgh-3NLS
Plasmid#194470PurposeEncodes for catalytically inactive bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.DepositorInsertBtGH84
UseFor in vitro transcription from t3 promoter after…TagsSV40 Nuclear Localization Signal, SV40 Nuclear Lo…ExpressionMutationAspartic Acid 242 (in Dennis et al.) changed to A…PromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 921 - 940 of 14048 results