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Plasmid#174099PurposeAcridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.DepositorInsertAcridone 82 tRNA synthetase and tRNA
UseTagsExpressionBacterialMutationN311S, C313G, V366A, W382TPromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 PARL-FLAG-CT S65A+T69A+S70A
Plasmid#13616DepositorUseTagsFLAGExpressionMammalianMutationchanged Ser 65, Thr 69 and Ser 70 to AlaninePromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLenti_C_Myc_DDK_IRES_Puro_ALDH7A1
Plasmid#106120PurposeLentiviral expression of ALDH7A1DepositorInsertALDH7A1 (ALDH7A1 Human)
UseLentiviralTagsDDK (FLAG) and MycExpressionMutationPromoterAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHM130Env
Plasmid#137665PurposeExpress HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)DepositorInsertHIV-1 Envelope Glycoprotein
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgFFL
Plasmid#59877Purposesynthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene.DepositorInsertmCherry with intron containing the mouse mir-124–3
UseTagsPEST and VAMP 3 UTRExpressionMammalianMutationPromoterdoxycycline (Dox)-inducible promoter (CMV/TO)Available sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCI syn iGluf
Plasmid#106121PurposeFast indicator for synaptic glutamate imaging (synapsin promoter)DepositorInsertiGluSnFR E25D variant
UseTagsMyc and PDGFRExpressionMammalianMutationChanged Glu 25 to AspPromotersynapsinAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
myc-hRAD18 C207F
Plasmid#68829PurposeExpresses c-myc-tagged human RAD18 with C207F mutationDepositorInsertc-myc tagged human RAD18 with C207F mutation (RAD18 Human)
UseTagsc-mycExpressionMammalianMutationPromoterCAGAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pSG5-FLAG-CaMKKbeta rat RP deletion
Plasmid#32452DepositorInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
UseTagsFLAGExpressionMammalianMutationDeleted RP domain (amino acids 203-225 missing); …PromoterSV40Available sinceApril 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-Ef1a-mCh-Puro-Luci
Plasmid#31850DepositorInsertLuciferase RNAi
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterU6 for shRNA; Ef1alpha for mCherry-puromycinAvailable sinceJan. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Atoh1ER-IRES-GFP
Plasmid#33335DepositorInsertAtoh1 (Atoh1 Mouse)
UseRetroviralTagsERExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
LITE1.0_pAAV_hSyn_TALE(Grm2)-NLS-CIB1_2A_GFP_WPRE_bGHpA
Plasmid#47453PurposeepiLITE / LITE1.0 TALE-CIB1. CIB1 binds to blue-light-activated CRY2PHR. Particular TALE targeted to Grm2 promoter. Synapsin promoter for neuronal expression.DepositorInsertTALE (N136,Grm2,C63)-cib1
UseAAV; TaleTagsHAExpressionMammalianMutationPromoterhSyn human synapsin (mammalian neuronal expressio…Available sinceOct. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti_dCas9-2xAM_hIRF-1
Plasmid#92221PurposeLentiviral plasmid expressing dCas9-2xAM and gRNA for human IRF-1 promoterDepositorInsertsSp-dCas9-2xAM tag
gRNA targeting human IRF-1 promoter
UseCRISPR and LentiviralTags2xAM tagExpressionMammalianMutationhuman codon-optimized, D10A + H840APromoterCBh and U6Available sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRpr150-LUC
Plasmid#85700Purposeluciferase reporter driven by a DNA damage responsive cis-regulatory sequence from the reaper locus, containing a p53 binding site (Rpr150 enhancer)DepositorInsertRpR 150 enhancer and hsp70 minimal promoter
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterRpR 150 enhancer and hsp70 minimal promoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_TALEBB(HD)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA
Plasmid#47450PurposeAAV TALE synthesis backbone with HD half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.DepositorInsertTALE BB(N136_0.5HD_C63)-SID4X
UseAAV; TaleTags2A_phiLOV2.1ExpressionMammalianMutationPromoterhSyn human synapsin (mammalian neuronal expressio…Available sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro KR ATG3
Plasmid#26927DepositorInsertautophagy-related 3 (yeast) (Atg3 Mouse)
UseRetroviralTagsHis and V5ExpressionMammalianMutationChanged Lysine 243 to Arginine.PromoterAvailable sinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-Arl13b17
Plasmid#40875DepositorInsertArl13b (Arl13b Mouse)
UseTagsGFPExpressionMammalianMutationcontains Arl13b amino acids 1-199PromoterCMVAvailable sinceMarch 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRP(Exp)-CMV> ORF_924bp (Azgp1):dTomato:IRES:Puro
Plasmid#110831PurposeExpresses both zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter, where the fluorescent dT reporter is fused with the ZAG protein.DepositorInsertAzgp1 (Azgp1 Mouse)
UseNon-viral gene expression vectorTagsdTomatoExpressionMutationPromoterCMVAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMM1157
Plasmid#112534PurposepZE2-PLhrtO-lux-hrtR-RBS3 - ChuA transciptional unit deletion of plasmid pMM643, ColE1 origin, KanRDepositorInsertsHrtR
luxCDABE
UseTagsExpressionBacterialMutationPromoterPL(HrtO) and ProDAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM2-rPMCA3b
Plasmid#47763Purposemammalian expression of rat PMCA3bDepositorInsertPMCA3b (Atp2b3 Rat)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceSept. 3, 2013AvailabilityAcademic Institutions and Nonprofits only