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Plasmid#83494PurposeBacterial expression of L. buccalis C2c2 CRISPR effector (R1079A- pre-crRNA processing mutant)DepositorInsertLbu_C2c2_R1079A
UseCRISPRTagsHis6-MBPExpressionBacterialMutationR1079APromoterT7Available sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
PlmdCas12e KRAB
Plasmid#188506PurposeExpresses FLAG tagged PlmdCas12e KRABDepositorInsertdCas12e
UseCRISPR and LentiviralTagsNLS-FLAG-KRAB-NLSExpressionMammalianMutationD659A/E756A/D922APromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
FLAG-GAS41
Plasmid#15428DepositorInsertGAS41 (Yeats4 Mouse)
UseTagsFLAGExpressionMammalianMutationN/APromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4136464
Plasmid#149534PurposeBacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTags6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEVExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP-C-SLC35B2
Plasmid#155037PurposeTransient expression of EGFP-SLC35B2DepositorInsertSLC35B2 (SLC35B2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LINXb3
Plasmid#73615PurposeLight-induced nuclear export switch for microscopy in mammalian cells.DepositorInsertLINXb3
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS-45
Plasmid#40586DepositorUseTagsHis-tag and yTAND12ExpressionBacterialMutationPromoterT7Available sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
MAC-SARS-CoV-2 Orf6
Plasmid#158374PurposeMAC-tagged gene expressionDepositorInsertSARS-CoV-2 Orf6 (ORF6 )
UseTagsMAC-tagExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pH6HTC_ABCE1-Halo
Plasmid#175335PurposeProduction of HaloTag fusion proteinDepositorInsertABCE1 (ABCE1 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_VPR_Puro
Plasmid#167887PurposePiggyBac compatible plasmid expressing dCas9-VPRDepositorInsertdCas9-VPR
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tmem98-sGFP2
Plasmid#124503PurposeExpresses sGFP2-tagged mouse TMEM98 in mammalian cells (C-terminal tag)DepositorInsertTmem98 (Tmem98 Mouse)
UseTagssGFP2ExpressionMammalianMutationstop codon was removed to allow fusion with sGFP2…PromoterCMVAvailable sinceMay 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISP_IS
Plasmid#120425PurposeThis plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3 and IS5.DepositorInsertCRISPR spacers targeting IS1, IS5, IS3
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterconstitutiveAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-ECFP-GIT1
Plasmid#15223DepositorInsertG protein coupled receptor kinase-interacting protein 1 (GIT1 Human)
UseTagsECFP and flagExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro human lipin 1-1 shRNA
Plasmid#32019DepositorInsertlipin1 shRNA
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast SHMT1
Plasmid#106304PurposeExpress SHMT1DepositorInsertserine hydroxymethyltransferase 1 (SHMT1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
PE_CO-Mini-N
Plasmid#190336PurposeExpressing the N-terminal of PE_CO-Mini using Rma intein and Cas9 673-674 split site, ngRNA and pegRNA for PCSK9 +1A insertionDepositorInsertPE_CO-Mini-N, ngRNA and pegRNA for PCSK9 +1A insertion
UseAAVTagsExpressionMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMCS.71/2272
Plasmid#22732DepositorInsertLox71-MCS-Lox2272
UseCre/LoxTagsExpressionMutationThis is a basal exchange vector for RMCE containi…PromoterAvailable sinceDec. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
MAC-SARS-CoV-2 NSP1
Plasmid#158352PurposeMAC-tagged gene expressionDepositorInsertSARS-CoV-2 NSP1 (ORF1ab )
UseTagsMAC-tagExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pXFokI-dCas9
Plasmid#60901PurposeDual Expression Vector for FokI-dCas9 and gRNADepositorTypeEmpty backboneUseCRISPRTags3XFLAGExpressionMammalianMutationPromoterCBh and U6Available sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAC-SARS-CoV-2 E
Plasmid#158368PurposeMAC-tagged gene expressionDepositorInsertSARS-CoV-2 E (E )
UseTagsMAC-tagExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits