Addgene

We narrowed to 14,149 results for: LEA

Showing: 9301 - 9320 of 14149 results

Results per page:

pBXNH3A

Plasmid

#47075

Purpose

FX cloning E.coli expression vector with araBAD promoter and N-terminal 10x His, 3C protease cleavage site and Avi-tag

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), Avi-tag, and…

Expression

Bacterial

Mutation

Promoter

araBAD

Availability

Academic Institutions and Nonprofits only

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pREXC3GH

Plasmid

#47077

Purpose

FX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Lactococcus lactis expression

Tags

3C protease site, GFP, and His

Expression

Mutation

Promoter

NIS (nisin)

Availability

Academic Institutions and Nonprofits only

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pREXC3H

Plasmid

#47078

Purpose

FX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and C-terminal 3C protease cleavage site and 10x His tags

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Lactococcus lactis expression

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Mutation

Promoter

NIS (nisin)

Availability

Academic Institutions and Nonprofits only

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pREXNH3

Plasmid

#47079

Purpose

FX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Lactococcus lactis expression

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Mutation

Promoter

NIS (nisin)

Availability

Academic Institutions and Nonprofits only

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LV-H2B-mRFP1

Plasmid

#47376

Purpose

This was used as a nuclear marker

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

H2B

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pICXNHG3

Plasmid

#49021

Purpose

FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Yeast

Mutation

Promoter

AOX1

Availability

Academic Institutions and Nonprofits only

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pICXC3GH

Plasmid

#49023

Purpose

FX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tag

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Yeast

Mutation

Promoter

AOX1

Availability

Academic Institutions and Nonprofits only

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pcDXNSM3

Plasmid

#49028

Purpose

FX cloning mammalian cell line expression vector with CMV promoter and N-terminal streptavidin binding peptide, Myc tag and 3C protease cleavage site

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), MYC, and str…

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDXNSMG3

Plasmid

#49029

Purpose

FX cloning mammalian cell line expression vector with CMV promoter and N-terminal streptavidin binding peptide, Myc tag, EGFP and 3C protease cleavage site

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), EGFP, MYC, a…

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDXC3MS

Plasmid

#49030

Purpose

FX cloning mammalian cell line expression vector with CMV promoter and C-terminal 3C protease cleavage site, Myc tag and streptavidin binding peptide

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), MYC, and str…

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDXC3GMS

Plasmid

#49031

Purpose

FX cloning mammalian cell line expression vector with CMV promoter and C-terminal 3C protease cleavage site, EGFP, Myc tag and streptavidin binding peptide

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), EGFP, MYC, a…

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIC.B3.AF2373

Plasmid

#11529

Depositor

Sung-Hou Kim

Article

Liu et al J Mol Biol. 2005 Nov 25. 354(2):289-303.

Insert

NAD kinase (AF2373 A. fulgidus)

Use

Tags

HisTag, TEV cleavable

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pET15-UbE2D1

Plasmid

#15782

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2D1 (UBE2D1 Human)

Use

Tags

6x His, T7 tag, and Xpress tag (enterokinase clea…

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pSaI14

Plasmid

#8614

Depositor

Robert Hartley

Article

Krajcikova et al J Bacteriol 1998 Mar;180(6):1582-5.

Insert

Ribonuclease inhibitor SaI14

Use

Tags

Expression

Bacterial

Mutation

N terminal threonine changed to alanine

Promoter

Availability

Academic Institutions and Nonprofits only
pSaI20

Plasmid

#8615

Depositor

Robert Hartley

Article

Krajcikova et al J Bacteriol 1998 Mar;180(6):1582-5.

Insert

Ribonuclease inhibitor SaI20

Use

Tags

Expression

Bacterial

Mutation

N terminal threnine changed to alanine

Promoter

Availability

Academic Institutions and Nonprofits only

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4Z02

Plasmid

#69956

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

BRD1:JMC023-A05:C211705 (BRD1 Human)

Use

Tags

His and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7-lacO

Availability

Academic Institutions and Nonprofits only
5C5C

Plasmid

#69964

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

GRM7:DCC014-D04:C211307 (GRM7 Human)

Use

Baculovirus expression

Tags

His and TEV cleavage site

Expression

Mutation

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only
5C7M (ITCH)

Plasmid

#69966

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

ITCH:SDC094-D06:C29524 (ITCH Human)

Use

Tags

His and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7-lacO

Availability

Academic Institutions and Nonprofits only
Orai1 YFP

Plasmid

#19756

Depositor

Anjana Rao

Article

Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

Insert

Orai1 (ORAI1 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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Orai1 CFP

Plasmid

#19757

Depositor

Anjana Rao

Article

Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

Insert

Orai1 (ORAI1 Human)

Use

Tags

CFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge