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Plasmid#205191PurposeThis vector encodes of a synCAM (JAM-B) and GFPDepositorInsertsynCAM JAM-B and GFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-TAG-ERN
Plasmid#80476PurposeAll-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible GFP and constitutive rtTA and neomycin resistance)DepositorTypeEmpty backboneUsePiggybac transposonTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAS134
Plasmid#211505PurposeAAV genome to express Venus under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seqDepositorInsertVenus
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS132
Plasmid#211503PurposeAAV genome to express mCherry under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seqDepositorInsertmCherry
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE16
Plasmid#90357PurposeCREB - Cyclic AMP gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterCREBAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT-CA-GFP-IRES-mLumin
Plasmid#32749DepositorInsertsCA-GFP
mLumin
UseTagsExpressionMammalianMutationS158A and S65TPromoterCMVAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFP.E227
Plasmid#138249PurposeReporter plasmid - Expresses three fluorescent reporters from three different ECF promotersDepositorInsertsmCherry
Terminator
mTagBFP
Terminator
gfp
Terminator
UseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP17_up1691, P20_992, and iP16_3622Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
mGeos-M-Homer1-C-18
Plasmid#57528PurposeLocalization: Membrane Adaptor, Excitation: 503 / 569, Emission: 514 / 581DepositorInsertHomer1 (Homer1 Rat)
UseTagsmGeos-MExpressionMammalianMutationPromoterCMVAvailable sinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-hSYN-miRFP670nano3-WPRE-hGHpA
Plasmid#184688PurposeMammalian expression, miRFP670nano3DepositorInsertmiRFP670nano3
UseAAVTagsExpressionMutationPromoterhSYNAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST/hCx43-EGFP-N1
Plasmid#40907DepositorInsertConnexin 43 (GJA1 Human)
UseTagsEGFPExpressionMammalianMutationNo stop codon to create c-terminal fusionPromoterCMVAvailable sinceNov. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
OPEN HLA-A*02:01-BirA
Plasmid#215569PurposeE. coli expression of BirA tagged HLA-A*02:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.DepositorInsertOPEN HLA-A*02:01 BirA (HLA-A Human)
UseTagsBirAExpressionBacterialMutationGlycine 120 to CysteinePromoterT7Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Sumo TEV expression vector with BioBrick polypromoter restriction sites (14-S)
Plasmid#48313DepositorTypeEmpty backboneUseTagsHis6 and SumoExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RAB-EKARev
Plasmid#118474PurposeddRFP-based single-color biosensor for monitoring ERK activity.DepositorInsertRAB-EKARev
UseTags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hM3D(Gq)-mCherry
Plasmid#50476PurposeGq-coupled hM3D DREADD fused with mCherry under the control of CaMKIIa promoterDepositorUseAAVTagsmCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterCaMKIIaAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLentiCMVNeoDEST(705-1)-hAXL
Plasmid#124796PurposeExpresses AXL in mammalian cellsDepositorInsertAXL (AXL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
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DsRed-rab11 WT
Plasmid#12679DepositorInsertRAB11A (Homo sapiens) (RAB11A Human)
UseTagsDsRed and HAExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pIR-CMV-SCN2A-Variant-1-IRES-mScarlet
Plasmid#162279PurposeEukaryotic expression of human SCN2A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.DepositorInsertSCN2A Variant 1, stabilized (SCN2A Human)
UseTagsIRES-mScarletExpressionMammalianMutationIVS intron inserted after bp4551.PromoterCMVAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-BclXL-pEGFP-C1
Plasmid#177408PurposeTo express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-XL.DepositorInsertBcl-XL
UseTagsmCerulean3ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-FUSN-mCh-Cry2olig
Plasmid#101224PurposeFUS(1-214) fused to mCh-Cry2oligDepositorInsertFUS(aa1-214) (FUS Human)
UseLentiviralTagsmCherry-Cry2oligExpressionMammalianMutationPromoterSFFVAvailable sinceFeb. 1, 2018AvailabilityAcademic Institutions and Nonprofits only