Addgene

We narrowed to 16,102 results for: myc tag

Showing: 9281 - 9300 of 16102 results

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pcDNA3.1(-)_mtZFN(+)2G

Plasmid

#62798

Purpose

mtZFN2G architecture bearing (+) FokI domain

Depositor

Michal Minczuk

Article

Gammage et al EMBO Mol Med. 2014 Apr;6(4):458-66. doi:

Insert

mtZFN(+)2G

Use

Tags

Internal HA tag immediately after F1B mitochondri…

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)_mtZFN(-)2G

Plasmid

#62799

Purpose

mtZFN2G architecture bearing (-) FokI domain

Depositor

Michal Minczuk

Article

Gammage et al EMBO Mol Med. 2014 Apr;6(4):458-66. doi:

Insert

mtZFN(-)2G

Use

Tags

Internal FLAG tag immediately after F1B mitochond…

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 HA-rnSOX9 ΔC (1-381)

Plasmid

#62973

Purpose

Expresses HA-tagged rat SOX9 residues 1-383

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

SOX9 (Sox9 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

codes for residues 1-381

Promoter

Availability

Academic Institutions and Nonprofits only
pLEGFP-WT-STAT3

Plasmid

#71450

Purpose

Retroviral expression of WT-STAT3. Please note that this plasmid contains WT-STAT3 tagged with FLAG, not GFP.

Depositor

George Stark

Article

Dasgupta et al J Biol Chem. 2014 Oct 31;289(44):30763-7

Insert

STAT3 (STAT3 Human)

Use

Retroviral

Tags

FLAG

Expression

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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Snap-Omp25

Plasmid

#69599

Purpose

Snap-tag substrates with two different fluorophores (red and green) sequentially to separately label young and old mitochondria

Depositor

David Sabatini

Article

Katajisto et al Science. 2015 Apr 17;348(6232):340-3. do

Insert

Snap-Omp25

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-ERK5kin

Plasmid

#65245

Purpose

Expression of HA tagged ERK5 kinase domain in mammalian cells

Depositor

Axel Ullrich

Article

Buschbeck et al J Biol Chem. 2002 Aug 16;277(33):29503-9

Insert

ERK5kin (MAPK7 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

deleted AA 410-817

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-ERK5 713

Plasmid

#65246

Purpose

Expression of HA tagged ERK5 AA 2-713 in mammalian cells

Depositor

Axel Ullrich

Article

Buschbeck et al J Biol Chem. 2002 Aug 16;277(33):29503-9

Insert

ERK5 713 (MAPK7 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

deleted AA 714-817

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pcDNA3-PTK7-VSV

Plasmid

#65250

Purpose

Expression of VSV tagged PTK7 in mammalian cells

Depositor

Axel Ullrich

Article

Gartner et al PLoS One. 2014 Jan 7;9(1):e84472. doi: 1

Insert

PTK7 (PTK7 Human)

Use

Tags

VSV

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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VAMP2-12aa-mCit

Plasmid

#53237

Purpose

Fluorescent probe: SNAP-25B, C-terminally-tagged with monomeric Citrine, connected with a 12 amino acid linker. Expressed in mammalian cells, used with Stx1a-12aa-mCer to measure FRET and dispersion.

Depositor

Robert Zucker

Article

Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

Insert

VAMP2 (Vamp2 Rat)

Use

Tags

12aa-linker and mCitrine

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pCI-neo-alpha4-SEP

Plasmid

#45607

Depositor

Henry Lester

Article

Richards et al J Biol Chem. 2011 Sep 9;286(36):31241-9.

Insert

nAChRAlpha4-SEP

Use

Tags

SEP- Tag

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCLC3sFlag

Plasmid

#52422

Purpose

mammalian expression of flag tagged rat CLC3 short transcript

Depositor

Steven Weinman

Article

Shimada et al Am J Physiol Gastrointest Liver Physiol.

Insert

CLC3 (Clcn3 Rat)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pET28a-ybbR-HIS-Xyl-DocI

Plasmid

#58711

Purpose

Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

Depositor

Hermann Gaub

Article

Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

Insert

Xyl-DocI

Use

Tags

HIS, HRV 3C, and ybbR

Expression

Bacterial

Mutation

changed Threonin 120 to Cysteine in Xylanase Doma…

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pBABE-3xFLAG-OB D166R

Plasmid

#79061

Purpose

Retroviral expression of 3xFLAG tagged, nuclear localized TPP1-OB-RR folding domain

Depositor

Steven Artandi

Article

Zhong et al Cell. 2012 Aug 3;150(3):481-94. doi: 10.

Insert

TPP1 (TPP1 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Aspartic acid 166 changed to Arginine

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pcDNA3/mArf1(Q71L)-HA

Plasmid

#79410

Purpose

Expresses C-terminally HA-tagged Arf1(Q71L) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

Insert

Arf1 (Arf1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

changed Glutamine 71 to Leucine

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3/mArf1(T31N)-HA

Plasmid

#79411

Purpose

Expresses C-terminally HA-tagged Arf1(T31N) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

Insert

Arf1 (Arf1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

changed Threonine 31 to Asparagine

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3/mArf3(Q71L)-HA

Plasmid

#79415

Purpose

Expresses C-terminally HA-tagged Arf3(Q71L) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

Insert

Arf3 (Arf3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

changed Glutamine 71 to Leucine

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3/mArf3(T31N)-HA

Plasmid

#79416

Purpose

Expresses C-terminally HA-tagged Arf3(T31N) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

Insert

Arf3 (Arf3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

changed Threonine 31 to Asparagine

Promoter

Availability

Academic Institutions and Nonprofits only

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Lyn11-CIBN-GFP

Plasmid

#79572

Purpose

expression of plasma membrane signal from Lyn11 fused to CIBN and tagged with GFP

Depositor

Olof Idevall-Hagren, Pietro De Camilli

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

Lyn11

Use

Tags

CIBN (CRY2-binding domain) and EGFP

Expression

Mammalian

Mutation

G8R (please see depositor comments below)

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pCINeoFlag ALIX

Plasmid

#89859

Purpose

Expresses full length Flag-tagged ALIX in mammalian cells; internal ID WISP03-308

Depositor

Wesley Sundquist

Article

Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

Insert

ALIX (PDCD6IP Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-STX17ΔNTD

Plasmid

#89941

Purpose

Expresses syntaxin17 lacking N-terminal domain tagged with GFP in mammalian cells

Depositor

Noboru Mizushima

Article

Uematsu et al Autophagy. 2017 Aug 3;13(8):1452-1464. d

Insert

human syntaxin17 lacking N-terminal domain (STX17 Human)

Use

Retroviral

Tags

GFP

Expression

Mutation

deleted amino acids 1-161

Promoter

Availability

Academic Institutions and Nonprofits only

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