Addgene

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Showing: 9241 - 9260 of 24657 results

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pQHA-USP7 CS puroR

Plasmid

#46754

Purpose

Retroviral vector that expresses catalytically inactive form of HA-tagged human USP7

Depositor

Goedele Maertens, Gordon Peters

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP7 (USP7 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

C223S--catalytically inactive

Promoter

CMV

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pSb_init containing the TM287/288 nanobody Nb_TM#2

Plasmid

#132698

Purpose

expresses the TM287/288 nanobody Nb_TM#2 in E.coli, to use as positive control in ELISA

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

nanobody Nb_TM#2

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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NLS-GI-VP64-IRES-NLS-GAL4DBD-M13-SmBiT-FKF1

Plasmid

#183400

Purpose

Dual expression of GI-VP64 and GAL4DBD-M13-SmBiT-FKF1 in the nucleus

Depositor

Alice Ting

Article

Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

Insert

GI-VP64-IRES-GAL4DBD-M13-SmBiT-FKF1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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Myc-Axin2

Plasmid

#21279

Depositor

Frank Costantini

Article

Jho et al Mol Cell Biol. 2002 Feb . 22(4):1172-83.

Insert

Axin2 (Axin2 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2009

Availability

Academic Institutions and Nonprofits only
pTS99 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-3C-MCS

Plasmid

#199352

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-3C-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagBFP-P2A-ALFA-3C

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc3-CUL4A delta N52

Plasmid

#19952

Depositor

Yue Xiong

Article

Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

Insert

CUL4A (cullin 4A) (CUL4A Human)

Use

Tags

myc3

Expression

Mammalian

Mutation

Missing 52 residues from N terminal CUL4A.

Promoter

Available since

April 1, 2009

Availability

Academic Institutions and Nonprofits only
pTS110 pHAGE2 CMVtetO2 MCS-22xGS-Alfa-P2A-TagBFP

Plasmid

#199363

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally ALFA-P2A-BFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

ALFA-P2A-TagBFP

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTS111 pHAGE2 CMVtetO2 MCS-3C-22xGS-Alfa-P2A-TagBFP

Plasmid

#199364

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-ALFA-P2A-BFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

3C-ALFA-P2A-TagBFP

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TORCAR-NES

Plasmid

#175015

Purpose

Cytosol-targeted TORCAR.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

TORCAR-NES

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGDP4 tet(A)

Plasmid

#112887

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.

Depositor

Gerard Wright

Article

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

tet(A)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Sequence was codon optimized for E. coli K12 usin…

Promoter

Plac

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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PE_CO-Mini-C

Plasmid

#190337

Purpose

Expressing the C-terminal of PE_CO-Mini using Rma intein and Cas9 673-674 split site, and pegRNA for PCSK9 +1A insertion

Depositor

Rasmus Bak

Article

Gao et al Mol Ther. 2022 Jul 8. pii: S1525-0016(22

Insert

PE_CO-Mini-C, PCSK9 +1A insertion pegRNA

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 NSP9

Plasmid

#158360

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP9 (ORF1ab )

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pSilencer2.1-U6-3JB1F+2

Plasmid

#190833

Purpose

For mammalian expression of 3JB1F+2, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.

Depositor

Gerald Hart

Article

Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

Insert

3JB1F_T1+2bp (A Dual-Specificity aptamer targeting OGT and GFP)

Use

Affinity Reagent/ Antibody and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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GFP-N2-PKCgamma

Plasmid

#21204

Depositor

Tobias Meyer

Article

Oancea et al J Cell Biol. 1998 Feb 9. 140(3):485-98.

Insert

PKC gamma (Prkcg Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

PKC gamma (rat). (Lab plasmid WC 0013)

Promoter

Available since

July 15, 2009

Availability

Academic Institutions and Nonprofits only

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pEGFP-eDHFR-MCS

Plasmid

#172108

Purpose

Mammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFR

Depositor

Shinya Tsukiji

Article

Ishida et al J Am Chem Soc. 2013 Aug 28;135(34):12684

Insert

EGFP-eDHFR-20aa (folA E. coli)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-GFP-G3BP2(2)

Plasmid

#136061

Purpose

G3BP2 gRNA (#2) inserted into the pSpCas9(BB)-2A-GFP plasmid (GACAACTACTCCATCACTCA)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP2 (G3BP2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-HBB-nLuc

Plasmid

#175432

Purpose

Used to generate IVT RNA for translation extracts

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

HBB-nLuc

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pNP1 eforRed toehold switch

Plasmid

#107353

Purpose

Expresses eforRed (an RFP derivative) in Ecoli off a T7 promoter when pNP1 eforRed trigger is co-expressed

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

toehold switch 1 + eforRed

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pET16b-Trx CC32/35SS

Plasmid

#36946

Depositor

Anna Blom

Article

King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

Insert

Thioredoxin 1 (TXN Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

both active site cysteines (32,35) mutated to ser…

Promoter

T7

Available since

May 20, 2013

Availability

Academic Institutions and Nonprofits only
MAC-SARS-CoV-2 Orf7a

Plasmid

#158375

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf7a (ORF7a )

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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