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We narrowed to 8,122 results for: sgRNA

Showing: 901 - 920 of 8122 results

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pT7-SpCas9_sgRNA-site2 (RTW448)

Plasmid

#160137

Purpose

T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2

Depositor

Benjamin Kleinstiver, Keith Joung

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

SpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)

Use

In vitro transcription of sgrna from t7 promoter

Tags

Expression

Mutation

Promoter

T7

Available since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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lenti-sgRNA(MS2)_zeo_hPDX1_promoter_guide1

Plasmid

#176838

Purpose

To induce human PDX1 expression by recruiting SAM complex to PDX1 promoter

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

sgPDX1

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-scramble

Plasmid

#62285

Purpose

expression of scramble sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

scramble sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pX330-NQL007-NANOG-sgRNA

Plasmid

#175555

Purpose

For transient expression of spCas9-nuclease and a sgRNA targeting the mouse NANOG locus.

Depositor

Elzo de Wit

Article

Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

Insert

spCas9-nuclease and sgRNA against mouse NANOG STOP Codon

Use

Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(RBSmut)_Psyn-sgRNAmreB

Plasmid

#149594

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi mreB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAmreB

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30, Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Eif4a1-R

Plasmid

#122345

Purpose

Expresses sgRNA targeting mouse Eif4a1 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Eif4a1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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SauCas9KKH CCR5-nicking sgRNA

Plasmid

#169863

Purpose

SauCas9KKH nicking sgRNA for CCR5

Depositor

Scot Wolfe

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

SauCas9KKH CCR5-nicking sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6 promoter

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - Amplicon, JAK2 sgRNA 1

Plasmid

#70679

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backbone

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

sgRNA against JAK2 amplicon found in AML-derived HEL cell line (JAK2 )

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only
pM117: VEGFA array presgRNA

Plasmid

#166869

Purpose

U6-driven expression of human VEGFA targeting array presgRNA compatible with CasRx.

Depositor

Guei-Sheung Liu

Article

Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

Insert

Human VEGFA targeting array presgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(RBSmut)_Psyn-sgRNArodA

Plasmid

#149656

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30, Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-PpU6P-sgRNA-L5L2

Plasmid

#113738

Purpose

Gateway entry vector containing attL5 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

PpU6P::sgRNA

Use

CRISPR; Gateway entry vector

Tags

Expression

Mutation

Promoter

P. patens U6 promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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Cas9 ROSA sgRNA mCherry

Plasmid

#155280

Purpose

Lentiviral Cas9 expression, ROSA sgRNA expression, mCherry marker

Depositor

Martin Carroll

Insert

spCas9

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-Sa sgRNA expression vector

Plasmid

#107720

Purpose

For in vitro transcription of Sa sgRNA from the T7 promoter.

Depositor

Xingxu Huang

Article

Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A2T

Plasmid

#62258

Purpose

expression of A2T sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A2T sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Eif4a1-L

Plasmid

#122346

Purpose

Expresses sgRNA targeting mouse Eif4a1 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Eif4a1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCF820_U6-sgRNA-EF1a-mCherry2_lenti

Plasmid

#138316

Purpose

Lenti expression of mCherry2 with U6 promoter for SpyCas9 sgRNA targeting GFP

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

SpyCas9 GFP sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - Amplicon, JAK2 sgRNA 2

Plasmid

#70660

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backbone

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

sgRNA against JAK2 amplicon found in AML-derived HEL cell line (JAK2 )

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only
pAN-PBAD-sgRNA-A1T

Plasmid

#62256

Purpose

expression of A1T sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A1T sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR-PpU6P-sgRNA-R4R3

Plasmid

#113739

Purpose

Gateway entry vector containing attR4 and attR3 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

PpU6P::sgRNA

Use

CRISPR; Gateway entry vector

Tags

Expression

Mutation

Promoter

P. patens U6 promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-PpU6P-sgRNA-L3L2

Plasmid

#113740

Purpose

Gateway entry vector containing attL3 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

PpU6P::sgRNA

Use

CRISPR; Gateway entry vector

Tags

Expression

Mutation

Promoter

P. patens U6 promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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