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Plasmid#154431PurposePlasmid used to express the tVYN-Nt∆ luminescent biosensor for use in live cell cyclic di-GMP detectionDepositorInsertVenusΔC12-NtYcgRΔ-NLucΔ4
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
CyPet-FKBP-E2
Plasmid#175244PurposeBacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycinDepositorInsertUBE2I (UBE2I Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM41-EcLonA
Plasmid#38330DepositorInsertLon protease fragment (lon Escherichia coli)
UseTagsHis, MBP, and TEVExpressionBacterialMutationPolyphosphate binding fragment of Lon protease. …PromoterT7Available sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pet11a-ABLE
Plasmid#158627PurposeAmp resistant IPTG inducible 6xHis-TEV-ABLE protein for E. coli expressionDepositorInsertApixaban-Binding Helical Bundle
UseTags6x His-tag and TEV cleavage tagExpressionBacterialMutationPromoterT7Available sinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-BoNT/E
Plasmid#168020PurposeExpresses BoNT/E-LC(1-411)-6xHis in bacterial cellsDepositorInsertBoNT/E-LC(1-411)-6xHis
UseTagsHisx6ExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a Gx-NL2
Plasmid#169107PurposeExpresses protein G fused to 2 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - NanoLuc - NanoLuc
UseTagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7Available sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-UVRAG CC
Plasmid#24400DepositorInsertUVRAG (UVRAG Human)
UseTagsHisExpressionBacterialMutationContains on the CC domain, amino acids 200-270PromoterAvailable sinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET24c(+)-mtEFTu
Plasmid#30535DepositorInsertMature bovine mitochondrial elongation factor Tu
UseTagsHisExpressionBacterialMutationMature form amino acids 44-452. Mito import seque…PromoterAvailable sinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET-3c-rhVTN-C
Plasmid#30224DepositorInsertrhVitronectin with C terminus cleavage (VTN Human)
UseTagsExpressionBacterialMutationdeleted amino acids: 399-478PromoterAvailable sinceMay 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET28a-BCA-P(11)4
Plasmid#185780PurposeEncodes enzyme bovine carbonic anhydrase linked to P(11)4 self-assembling peptide via a GS-linker sequenceDepositorInsertBCA-P(11)4
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HA-CPDSalI
Plasmid#38254DepositorTypeEmpty backboneUseTags6xHis, HA, and Vibrio cholerae MARTX toxin cystei…ExpressionBacterialMutationPromoterT7Available sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SRAP
Plasmid#84524PurposeExpresses murine SRAP domain (250 aa) in bacteriaDepositorInsertSRAP domain (Hmces Mouse)
UseTags6xHis and S-TagExpressionBacterialMutationPromoterT7Available sinceNov. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET30-2-GPD1L
Plasmid#83912Purposestable overexpressionDepositorInsertglycerol-3-phosphate dehydrogenase 1-like (GPD1L Human)
UseTags6x His tagsExpressionBacterialMutationPromoterT7Available sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTS2353_Tier3(PB)-YPet_PuroR
Plasmid#169652PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven YPet and PuroR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_S-Luc_CLIP
Plasmid#113923PurposePlasmid encoding the bacterial expression of the chimeric SNAP-NanoLuc fusion dubbed S-Luc c-terminally linked to CLIP-tagDepositorInsertS-Luc_CLIP
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
CYPet-H2B-6
Plasmid#55461PurposeLocalization: Nucleus/Histones, Excitation: 433, Emission: 475DepositorInsertH2B (H2BC11 Human)
UseTagsCYPetExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceOct. 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET22b-HA-CPDSalI
Plasmid#38253DepositorTypeEmpty backboneUseTags6xHis, HA, and Vibrio cholerae MARTX toxin cystei…ExpressionBacterialMutationPromoterT7Available sinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET-CV-VAMP2
Plasmid#168023PurposeExpresses eCFP-VAMP2(T27-R86)-mVenus in bacterial cellsDepositorInserteCFP-VAMP2(T27-R86)-mVenus
UseTagsHisx6ExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ubp8/PET32a
Plasmid#26965DepositorInsertUbp8 (UBP8 Budding Yeast)
UseTagsHis6 and TrxExpressionBacterialMutationTEV site is added t the 5' end and 4 alanine…PromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET23a-MmRPS27a
Plasmid#69559PurposeBacterial expression; in vitro transcription/translation (T7)DepositorInsertRPS27A (RPS27A Human, Mouse)
UseTagsExpressionBacterialMutationChanged Alanine 133 (human RPS27a) to Glycine (mo…PromoterT7Available sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only