Addgene

We narrowed to 23,500 results for: pET

Showing: 901 - 920 of 23500 results

Results per page:

Sort results by:

pCEP4YPet-MAMM

Plasmid

#14032

Depositor

Patrick Daugherty

Article

Nguyen et al Nat Biotechnol. 2005 Mar . 23(3):355-60.

Insert

YPet

Use

Tags

Expression

Mammalian

Mutation

Mammalian optimized YPet

Promoter

Available since

Feb. 14, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pET32a-DEST

Plasmid

#84652

Purpose

E. coli expression vector derived from pET32 with Gateway cassette.

Depositor

Neil Olszewski

Type

Empty backbone

Use

Tags

His, S tag, and Trx Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM11-ACS

Plasmid

#108943

Purpose

heterologous expression of ACS of E. coli in E. coli

Depositor

Frank Schulz

Article

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Acetyl-CoA synthetase (ACS)

Use

Tags

His6-Tag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET14-Encap

Plasmid

#86405

Purpose

Expresses T. maritima encapsulin in E. coli

Depositor

David Savage

Article

Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

Insert

Encapsulin

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET23a-mUbc9

Plasmid

#53137

Purpose

E.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

Ubc9 (Ube2i Mouse)

Use

Tags

untagged

Expression

Bacterial

Mutation

protein sequence is identical to human

Promoter

T7

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pET24b-BR1.4

Plasmid

#199436

Purpose

expresses BAmRed1.4 in bacterial cells

Depositor

Andrew Woolley

Article

Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

Insert

BAMRed1.4

Use

Tags

his tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET22HT-PP7delFG

Plasmid

#31863

Depositor

Robert Singer

Article

Chao et al Nat Struct Mol Biol. 2008 Jan;15(1):103-

Insert

PP7delFG Coat Protein

Use

Tags

His6-tag and TEV site

Expression

Mutation

deleted FG loop

Promoter

Available since

Aug. 31, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pET22b-CPDSalI

Plasmid

#38251

Depositor

Aimee Shen, Matthew Bogyo

Article

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Type

Empty backbone

Use

Tags

6xHis and Vibrio cholerae MARTX toxin cysteine pr…

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pET30(a)-AtNUDX23

Plasmid

#175021

Purpose

Purification of AtNUDX23 enzyme

Depositor

Jeppe Vinther

Article

Sherwood et al Nature. 2023 Jul;619(7971):811-818. doi:

Insert

AtNUDX23 (NUDX23 Mustard Weed)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pETcLIC-GFP

Plasmid

#183514

Purpose

The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in E. coli

Depositor

Martin Högbom

Article

Grave et al Protein Expr Purif. 2022 Oct;198:106132.

Type

Empty backbone

Use

Tags

10x His-tag and folding reporter GFP

Expression

Bacterial

Mutation

Promoter

T7 lac

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET30 GLTP

Plasmid

#170733

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Malinina et al Nature. 2004 Aug 26;430(7003):1048-53. d

Insert

GLTP (GLTP Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET_sfCherry(1-10)_32aalinker_sfCherry(11)

Plasmid

#83030

Purpose

Bacterial expression vector for split sfCherry screening

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry(1-10)_32aalinker_sfCherry(11)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDB179-TurboPETase

Plasmid

#218693

Purpose

Encodes codon optimized TurboPETase enzyme for bacterial expression

Depositor

Gregg Beckham

Insert

TurboPETase

Use

Tags

10xHis

Expression

Bacterial

Mutation

Promoter

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
eSrtA(4S-9) pET29b

Plasmid

#75146

Purpose

reprogrammed sortase A variant that recognizes alternative substrate LPXSG with high activity and specificity

Depositor

David Liu

Article

Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

reprogrammed sortase A variant

Use

Tags

6x His

Expression

Bacterial

Mutation

N98D S102C A104V A118T F122A K134R F144L I182V E1…

Promoter

T7

Available since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_Pro-IL-36γ

Plasmid

#214345

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL36G (IL36G Human)

Use

Tags

His-TEV and Myc

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-MSP1D1delltaH4

Plasmid

#71713

Purpose

Membrane scaffold protein (MSP), helix 4 deletion

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET24b-Amg2

Plasmid

#199434

Purpose

expresses Amg2 in bacterial cells

Depositor

Andrew Woolley

Article

Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

Insert

Amg2

Use

Tags

his tag

Expression

Bacterial

Mutation

L159K mutation

Promoter

T7

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Pet22b-mEGFP

Plasmid

#166439

Purpose

Bacterial expression of 6x His tagged mEGFP

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET30a-FluB-NP

Plasmid

#165093

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Influenza B virus(B/Georgia/05/2019) nucleocapsid protein

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 11, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pET28b-PaODH

Plasmid

#110802

Purpose

Expresses PaODH and opine dehydrogenase from Pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

McFarlane et al Biochemistry. 2017 Nov 14;56(45):5967-59

Insert

Pa4835

Use

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge