-
Plasmid#55172Purposelux-reporter vector, integration at sacA, ampr, cmrDepositorTypeEmpty backboneUseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoternoneAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only
-
ku70 insUP+pPGI+NAT+tNOS+ku70 insD (SBE217)
Plasmid#195049Purposeintegration cassette for ku70 deletion and expression of NAT gene under pPGI for characterizationDepositorInsertNAT
UseTagsExpressionBacterialMutationPromoterpPGIAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
eforRed chromoprotein
Plasmid#117837PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses eforRed chromoprotein in E. coliDepositorInsertpromoter, RBS, eforRed
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
cjBlue chromoprotein
Plasmid#117844PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses cjBlue chromoprotein in E. coliDepositorInsertpromoter, RBS, cjBlue
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
meffRed chromoprotein
Plasmid#117836PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses meffRed chromoprotein in E. coliDepositorInsertpromoter, RBS, meffRed
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
amilCP_Pink chromoprotein
Plasmid#117851PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses amilCP_Pink chromoprotein in E. coliDepositorInsertpromoter, RBS, amilCP_Pink
UseSynthetic Biology; Escherichia coliTagsExpressionMutation64A,65C, BioBrick sites removedPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS4S
Plasmid#55170PurposeEmpty vector, integration at thrC, ampr, specrDepositorTypeEmpty backboneUseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoternoneAvailable sinceAug. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV_Npu-ABEmax C-terminal
Plasmid#137174PurposeExpresses the C-terminal split-intein fragment of ABEmax from the CMV promoterDepositorInsertNpu-ABEmax_C-terminal
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV_Npu-BE3.9max C-terminal
Plasmid#137172PurposeExpresses the C-terminal split-intein fragment of BE3.9max from the CMV promoterDepositorInsertNpu-BE3.9max_C-terminal
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-ligase-NLS-cTF
Plasmid#87746PurposeIPTG-inducible expression of ligase-NLS-cTF fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
UseTags6xHis and NLS linker - cTF (chimeric transcriptio…ExpressionBacterialMutationPromoterT5-lacAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
promoter J23110 (upstream of RBS, mRFP1)
Plasmid#117852PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertmedium-high promoter, RBS, mRFP1
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPPC001
Plasmid#171138PurposeFor integration of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T methodDepositorInsertSp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPRTagsExpressionBacterialMutationSoxS has R93A and S101A mutationsPromoterSp.pCas9, BBa_J23107Available sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
(K) tNOS (S)_T3 (SBE120)
Plasmid#195010Purposeterminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T3 position (K/S)DepositorInsert(K) tNOS (S)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
(H) tNOS (I) _T2 (SBE119)
Plasmid#195006Purposeterminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T2 position (H/I)DepositorInsert(H) tNOS (I)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
(B) HYG marker(R) (SBE264)
Plasmid#195013Purposehygromycin (HYG) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPDDepositorInsertpXYL+HYG+tGPD
UseTagsExpressionBacterialMutationPromoterpXYLAvailable sinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
(E) tNOS (F) _T1 (SBE118)
Plasmid#194997Purposeterminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T1 position (E/F)DepositorInsert(E) tNOS (F)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
(B) BLE marker(R) (SBE262)
Plasmid#195012Purposebleomycin (BLE) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPDDepositorInsertpXYL+BLE+tGPD
UseTagsExpressionBacterialMutationPromoterpXYLAvailable sinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
H446 EF1a RA-ABE6.3 p2A Puro
Plasmid#139104PurposeLentiviral expression of ABE6.3.DepositorInsertABE RA6.3
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZ_lacO-cP-AOX1-eGFP
Plasmid#126720Purposeyeast plasmid overexpressing eGFPDepositorInsertegfp
UseTags6xHis,MycExpressionYeastMutationPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPPC011
Plasmid#171143PurposeExpression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmidDepositorInsertSp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPRTagsExpressionBacterialMutationSoxS has R93A and S101A mutationsPromoterSp.pCas9, BBa_J23107Available sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only