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Plasmid#175451PurposeProtein expression/secretion in mammalian cells. CD44 Link module (exons 2-5), A20-P220. Can be biotinylated.DepositorInsertCD44 (CD44 Human)
UseTagsAviTag-His6ExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
CD44U-c011
Plasmid#175454PurposeProtein expression/secretion in mammalian cells. CD44 splice variant 10 (exons 2-5, 11, 15-16), A20-E649. Can be biotinylated.DepositorInsertCD44 (CD44 Human)
UseTagsAviTag-His6ExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CLK3_IDG-K
Plasmid#170579PurposeGateway destination clone of CLK3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CLK3 (CLK3 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-EEF2K_IDG-K
Plasmid#170592PurposeGateway destination clone of EEF2K (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-EEF2K (EEF2K Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceJuly 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CAMKV_IDG-K
Plasmid#158170PurposeGateway destination clone of CAMKV (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CAMKV (CAMKV Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-DYRK4_IDG-K
Plasmid#158184PurposeGateway destination clone of DYRK4 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-DYRK4 (DYRK4 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CDKL2_IDG-K
Plasmid#158181PurposeGateway destination clone of CDKL2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CDKL2 (CDKL2 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CDKL1_IDG-K
Plasmid#158176PurposeGateway destination clone of CDKL1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CDKL1 (CDKL1 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CAMK1G_IDG-K
Plasmid#158168PurposeGateway destination clone of CAMK1G (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CAMK1G (CAMK1G Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-BRSK2-V5-miniTurbo_IDG-K
Plasmid#135252PurposeGateway destination clone of BRSK2 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertBRSK2-V5-miniTurbo (BRSK2 Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-BRSK2_IDG-K
Plasmid#135271PurposeGateway destination clone of BRSK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-BRSK2 (BRSK2 Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-BRSK2-V5_IDG-K
Plasmid#135254PurposeGateway destination clone of BRSK2 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertBRSK2-V5 (BRSK2 Human)
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
AviTag-SpyTag003-MBP
Plasmid#184227PurposeExpresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.DepositorInsertAviTag-SpyTag003-MBP
UseTagsAviTag, His6, SpyTag003, and Thrombin cleavage si…ExpressionBacterialMutationPromoterT7Available sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
CD200RCD4d3+4-bio-linker-His
Plasmid#36153PurposeA positive control receptor bait with bio-linker-His (rat CD4d3+4-bio-linker-his) and prey (rat CD200 receptor) EXPRESs plasmidDepositorInsertCD200R (Cd200r1 Rat)
UseTagsHis tag, biotinylation peptide, and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailable sinceMay 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHL-avitag3
Plasmid#99847Purposemammalian expression with secretive signal and avi tag for biotinylationDepositorInsertephrin b2 (EPHB2 Human)
UseTagsAviExpressionMammalianMutationPromoterAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-AKT1-V5-miniTurbo_IDG-K
Plasmid#189889PurposeGateway destination clone of AKT1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertAKT1 (AKT1 Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-AKT1_IDG-K
Plasmid#189890PurposeGateway destination clone of AKT1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertAKT1 (AKT1 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMy-BirA-T2A-eGFP
Plasmid#50565PurposeRetroviral vector encoding for GFP and the biotinylating enzyme BirADepositorInsertAmpicilin
UseRetroviralTagsExpressionMutationPromoterAvailable sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-AviTag-DogCatcher-MBP
Plasmid#171928PurposeExpresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.DepositorInsertAviTag-DogCatcher-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationDogCatcher added to N-terminus of MBP via a GSGES…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-CDK11B-V5-miniTurbo_IDG-K
Plasmid#189914PurposeGateway destination clone of CDK11B (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertCDK11B (CDK11B Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only