CD200RCD4d3+4-bio-linker-His
(Plasmid
#36153)
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PurposeA positive control receptor bait with bio-linker-His (rat CD4d3+4-bio-linker-his) and prey (rat CD200 receptor) EXPRESs plasmid
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 36153 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTT3
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Backbone manufacturerYves Durocher, NRC
- Backbone size w/o insert (bp) 6000
- Total vector size (bp) 7340
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCD200R
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Alt nameOX-2
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SpeciesR. norvegicus (rat)
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Insert Size (bp)714
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Entrez GeneCd200r1 (a.k.a. Mox2r)
- Promoter CMV
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Tags
/ Fusion Proteins
- ratCD4d3+4 (C terminal on backbone)
- biotinylation peptide (C terminal on backbone)
- His tag (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Resource Information
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Addgene Notes
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A portion of this plasmid was derived from a plasmid made bypTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CD200RCD4d3+4-bio-linker-His was a gift from Gavin Wright (Addgene plasmid # 36153 ; http://n2t.net/addgene:36153 ; RRID:Addgene_36153) -
For your References section:
Large-scale screening for novel low-affinity extracellular protein interactions. Bushell KM, Sollner C, Schuster-Boeckler B, Bateman A, Wright GJ. Genome Res. 2008 Apr;18(4):622-30. Epub 2008 Feb 22. 10.1101/gr.7187808 PubMed 18296487