Addgene

166,242 results

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eTd-CBE

Plasmid

#196601

Purpose

Mammalian expression of eTd-CBE

Depositor

Dali Li

Article

Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

Insert

bpNLS-TadA-8e (N46L)-nSpCas9-bpNLS-P2A-UGI-bpNLS

Use

Tags

6*His

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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Lamp1-mCherry-RUSH

Plasmid

#202799

Purpose

RUSH cargo: Str-KDEL_IRES_SBP-mCherry-Lamp1

Depositor

Juan Bonifacino

Article

Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

Insert

Lamp1 (Lamp1 Rat)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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GP736

Plasmid

#164532

Purpose

Express Smo-FLAG-Ub

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened (Smo Mouse)

Use

Lentiviral

Tags

FLAG-Ub

Expression

Mutation

WT

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-EGFP_Hu_p115-RhoGEF(WT)

Plasmid

#136337

Purpose

Mammalian expression of human p115-RhoGEF with N-terminal EGFP

Depositor

Marcos Malumbres, Guillermo de Cárcer

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

ARHGEF1 (ARHGEF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-TID-HA3-KanMX6

Plasmid

#227980

Purpose

To insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, KanMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pBBR1k-GFPuv

Plasmid

#106382

Purpose

IPTG-inducible trc promoter expressing gfpuv on BBR1 origin

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Use

Tags

Expression

Bacterial

Mutation

Promoter

trc

Available since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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TFORF2260

Plasmid

#141998

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SOX17 (SOX17 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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FUGW-VGLUT2-pHluorin

Plasmid

#207469

Purpose

Lentiviral expression of VGLUT2-pHluorin

Depositor

Robert Edwards

Article

Silm et al Neuron. 2019 May 22;102(4):786-800.e5. d

Insert

VGLUT2 (Slc17a6 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pRiA4-VIR

Plasmid

#138193

Purpose

Virulence helper compatible with pCambia binary vectors.

Depositor

Qi-Jun Chen

Article

Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

Insert

vir gene cluster

Use

Vir gene expression

Tags

Expression

Mutation

Promoter

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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Peft-3::cas9-SV40_NLS::tbb-2 3'UTR

Plasmid

#46168

Depositor

John Calarco

Article

Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

Insert

codon optimized Cas9_SV40 NLS with intron

Use

CRISPR

Tags

Expression

Mutation

intron inserted at position 3113-3163, and SV40 N…

Promoter

eef-1A.1 (eft-3)

Available since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro

Plasmid

#141386

Purpose

Lentiviral expression of SARS-CoV-2 membrane (M) protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Has Service

Lentiviral Prep

Insert

SARS-CoV-2-M (M SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCIneo-hCHD7

Plasmid

#89458

Purpose

constitutive expression of human CHD7 in mammalian cells

Depositor

Peter Scacheri

Insert

CHD7 (CHD7 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR1A-Huwe1

Plasmid

#37431

Depositor

Jean Cook

Insert

Huwe1 (HUWE1 Human)

Use

Gateway entry

Tags

Expression

Mutation

Promoter

Available since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pHR GFP-CaaX

Plasmid

#113020

Purpose

for lentivirus production of GFP-CaaX

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

GFP-CaaX

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pUB-GFP

Plasmid

#11155

Purpose

Mammalian expression vector for expression of GFP (Ubiquitin C promoter)

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Has Service

Cloning Grade DNA

Insert

Ubiquitin C promoter (UBC Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pHR-EF1Alpha-puro-T2A-Tet-on 3G-TRE3G-Ascl1

Plasmid

#118593

Purpose

Expresses a puromycin resistance cassette with Tet-on 3G from the EF1Alpha promoter and Ascl1 from the TRE3G promoter

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

Puro-T2A-Tet-on 3G/Ascl1 (Ascl1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1Alpha/TRE3G

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMIEG3-JunDN

Plasmid

#40350

Depositor

Alexander Dent

Article

Wang et al J Immunol. 2005 Feb 15;174(4):2098-105.

Insert

Jun dominant negative (JUN Human)

Use

Retroviral

Tags

HA and IRES eGFP

Expression

Mammalian

Mutation

first 122 amino acids deleted

Promoter

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA-4mtD3cpv

Plasmid

#36324

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Chem Biol. 2006 May;13(5):521-30.

Insert

4mtD3cpv second generation cameleon (calcium sensor) targeted to mitochondria

Use

Tags

First 29 aa of human COX subunit 8a

Expression

Mutation

Promoter

CMV

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pBS_12x-601_MA1

Plasmid

#114358

Purpose

12x Widom 601 nucleosome sequence

Depositor

Scot Wolfe

Article

Swygert et al Proc Natl Acad Sci U S A. 2018 Dec 4;115

Insert

12X601-MA1

Use

Unspecified

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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p6XHis_NLS-SaCas9

Plasmid

#101086

Purpose

Express SaCas9 in bacteria with a 6xHis tag for purification

Depositor

Rick Tarleton

Article

Soares Medeiros et al MBio. 2017 Nov 7;8(6). pii: e01788-17. d

Insert

CRISPR-associated protein Cas9/Csn1

Use

CRISPR

Tags

TrxA-6xHis-STag

Expression

Bacterial

Mutation

Promoter

T7lac

Available since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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