-
Plasmid#210271PurposeN-terminal mCherry-tagging of inserts for inducible expression. Use with Flp-In T-Rex system.DepositorTypeEmpty backboneUseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only
-
px330-UFM1 sgRNA2
Plasmid#134634Purposecontains sgRNA targeting human UFM1 for gene knockoutDepositorInsertUFM1 sgRNA2 (UFM1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
px330-UFM1 sgRNA1
Plasmid#134633Purposecontains sgRNA targeting human UFM1 for gene knockoutDepositorInsertUFM1 sgRNA1 (UFM1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-Kdm6b(1025-End)-pA
Plasmid#100278PurposeIn vitro transcription template (T7 promoter) for Kdm6b catalytic domain active form.DepositorInsertKdm6b (Kdm6b Mouse)
UseTagsFlag epitope tagExpressionMammalianMutationcatalytic domain (1025aa-End)PromoterT7Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR H1-miR-30 L1-R5
Plasmid#62181PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEY12
Plasmid#191018PurposeFluorescent reporter driving panneuronal nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)DepositorInsertrab-3 (rab-3 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY46
Plasmid#191050Purposeglr-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInsertglr-1 (glr-1 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEY38
Plasmid#191042Purposeunc-8 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)DepositorInsertunc-8 (unc-8 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY14
Plasmid#191059Purposeacr-5 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertacr-5(orf) (acr-5 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY13
Plasmid#191019PurposeFluorescent reporter driving amphid and phasmid nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)DepositorInsertarrd-4 (ZK938.2 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY20
Plasmid#191026Purposegcy-18 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertgcy-18 (gcy-18 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY41
Plasmid#191045Purposedat-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInsertdat-1 (dat-1 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDAS12230_pegRNA-PEAR-GFP(10PBS-24RT)-mCherry
Plasmid#177182Purposeplasmid expressing a pegRNA targeting the PEAR-GFP plasmid along with an mCherry markerDepositorInsertpegRNA targeting the PEAR-GFP plasmid
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAOJ15
Plasmid#91567PurposeBroad host range allelic exchange suicide vector for bacteria - Tse2 toxin driven by anhydrotetracycline inducible promoterDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterTetracycline promoterAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-RNMT
Plasmid#112705PurposeExpresses N-terminally FLAG-tagged human RNMT in mammalian cellsDepositorInsertRNMT (RNMT Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE-SHH
Plasmid#134350PurposeTargeting vector for site specific integration of doxycycline inducible expression of full length human SHH cDNA into human AAVS1 locusDepositorInsertSHH (SHH Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hyg-RFPKDEL
Plasmid#138660PurposeReporter plasmid expressing the ER-located RFPDepositorInsertRFP-KDEL
UseTagsKDELExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-optimizedNedd4.2d
Plasmid#165103Purposebacterial expression of a GST fusion of Human Nedd4.2dDepositorInsertNedd4.2d (NEDD4L Human)
UseTagsGST from plasmid.ExpressionBacterialMutationcodon optimized for E. coli expressionPromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 human A3C
Plasmid#105047PurposeOverexpression of APOBEC3C in mammalian cell linesDepositorInsertAPOBEC3C (A3C) (APOBEC3C Human)
UseTagsHA tagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KanR neo
Plasmid#167869PurposePiggyBac compatible plasmid expressing dCas9DepositorInsertdCas9
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only