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Plasmid#216388Purposethis vector can be used to make modRNA expressing VP64dCas9VP64 and MPHDepositorInsertVP64dCas9VP64-2A-MPH
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-GCaMP6M (AAV8)
Viral Prep#137121-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-Ef1a-Coff/Fon-GCaMP6M (#137121). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Coff/Fon-GCaMP6M plasmid DNA. EF1a-driven expression of GCaMP6m in the presence of Flp recombinase (inhibited in the presence of Cre recombinase). These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsNoneAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP7b-WPRE (AAV Retrograde)
Viral Prep#104489-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pGP-AAV-syn-jGCaMP7b-WPRE (#104489). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7b-WPRE plasmid DNA. Syn-driven jGCaMP7b calcium sensor. jGCaMP7b exhibits the brightest resting fluorescence of the jGCaMP7 variants and can be used for imaging of small neuronal processes. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a_Flag_EGFP
Plasmid#183117PurposeExpresses flag-tagged EGFP in mammalian cellsDepositorInsertEGFP
UseLentiviralTagsFlagExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
-204 human IFN-gamma luc
Plasmid#17596DepositorInsertIFN gamma promoter (IFNG Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutation-204 to +38 of promoterPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
4MT-D3-CPV
Plasmid#58184PurposeLocalization: Mitochondria Ca Biosensor, Excitation: 433 / 515 , Emission: 475 / 528DepositorTypeEmpty backboneUseTagsECFP and VenusExpressionMammalianMutationPromoterAvailable sinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pETDuet-T7-Ct-YhjH
Plasmid#79167PurposeDual expression of Ct biosensor and YhjH under individual T7 promotersDepositorInsertsyhjH
T7-Spinach2-Ct
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMRX-No-HaloTag7-mGFP-LC3
Plasmid#184901PurposeStable expression of HaloTag7-mGFP-LC3 in mammalian cells to measure autophagic fluxDepositorInsertLC3B
UseTagsHaloTag7 and monomeric EGFPExpressionMammalianMutationPromoterAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG-mito-dsRED2
Plasmid#173071Purposemitochondria targeted dsRED2 expressed off of CAG promoterDepositorInsertMito
UseLentiviralTagsdsREDExpressionMammalianMutationPromoterCAGAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWS5397-CRISPRai-NatR
Plasmid#182836PurposeSaccharomyces cerevisiae inducible CRISPRai vector - NatR markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAK_DR30_CasRx_GFP
Plasmid#134843PurposeAll in one overexpression of CasRx and guideRNADepositorInsertRfxCas13d
UseCRISPRTagsT2A EGFPExpressionMammalianMutationPromoterCAGAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-mRuby2-FRET-10
Plasmid#58179PurposeLocalization: Cytosolic FRET, Excitation: , Emission:DepositorTypeEmpty backboneUseTagsmNeonGreen and mRuby2ExpressionMammalianMutationPromoterAvailable sinceMarch 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK170.AAV-TRE-Cre-WPRE (Supernova)
Plasmid#85040PurposeFor AAV-TRE-Cre based Supernova, pK170 should be used with pK168 etc.DepositorInsertnlsCre-WPRE
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-TagRFP
Plasmid#67167PurposeCRISPaint universal donor for TagRFP fusion taggingDepositorInsertTagRFP
UseTagsExpressionMutationPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBTK527a
Plasmid#110606PurposeBTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with spectinomycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
Bassik lab Human CRISPR-Cas9 Deletion Library - Drug targets, kinases, phosphatases
Pooled Library#101927PurposeBassik lab Human CRISPR-Cas9 Deletion Library - Drug targets, kinases, phosphatases. Coexpresses mCherry.DepositorExpressionMammalianUseCRISPRAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT/TO SF-HERC2 (ShB-R)
Plasmid#55613PurposeDox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged wild-type HERC2 in Flp-In TREx cellsDepositorInsert3xFLAG tagged HERC2 (WT) (HERC2 Human)
UseTags3xFLAG and STREPExpressionMammalianMutationSilent mutations have been incorporated into the …PromoterAvailable sinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYLsgRNA-AtU6-1
Plasmid#66202Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15p65
Plasmid#27661DepositorInsertTetrahymena telomerase holoenzyme p65
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-hygro-mCherry-RAMP4
Plasmid#118391Purposestable lentiviral expression of cDNADepositorInsertRAMP4 (SERP1 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterEIF-1a promoterAvailable sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only